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Open Access 01-12-2014 | Research

Splicing factor 3b subunit 1 (Sf3b1) haploinsufficient mice display features of low risk Myelodysplastic syndromes with ring sideroblasts

Authors: Valeria Visconte, Ali Tabarroki, Li Zhang, Yvonne Parker, Edy Hasrouni, Reda Mahfouz, Kyoichi Isono, Haruhiko Koseki, Mikkael A Sekeres, Yogen Saunthararajah, John Barnard, Daniel Lindner, Heesun J Rogers, Ramon V Tiu

Published in: Journal of Hematology & Oncology | Issue 1/2014

Abstract

Background

The presence of somatic mutations in splicing factor 3b subunit 1 (SF3B1) in patients with Myelodysplastic syndromes with ring sideroblasts (MDS-RS) highlights the importance of the RNA-splicing machinery in MDS. We previously reported the presence of bone marrow (BM) RS in Sf3b1 heterozygous (Sf3b1^+/−) mice which are rarely found in mouse models of MDS. Sf3b1^+/− mice were originally engineered to study the interaction between polycomb genes and other proteins.

Methods

We used routine blood tests and histopathologic analysis of BM, spleen, and liver to evaluate the hematologic and morphologic characteristics of Sf3b1^+/− mice in the context of MDS by comparing the long term follow-up (15 months) of Sf3b1^+/− and Sf3b1^+/+ mice. We then performed a comprehensive RNA-sequencing analysis to evaluate the transcriptome of BM cells from Sf3b1^+/− and Sf3b1^+/+ mice.

Results

Sf3b1^+/− exhibited macrocytic anemia (MCV: 49.5 ± 1.6 vs 47.2 ± 1.4; Hgb: 5.5 ± 1.7 vs 7.2 ± 1.0) and thrombocytosis (PLTs: 911.4 ± 212.1 vs 878.4 ± 240.9) compared to Sf3b1^+/+ mice. BM analysis showed dyserythropoiesis and occasional RS in Sf3b1^+/− mice. The splenic architecture showed increased megakaryocytes with hyperchromatic nuclei, and evidence of extramedullary hematopoiesis. RNA-sequencing showed higher expression of a gene set containing Jak2 in Sf3b1^+/− compared to Sf3b1^+/+.

Conclusions

Our study indicates that Sf3b1^+/− mice manifest features of low risk MDS-RS and may be relevant for preclinical therapeutic studies.

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Title: Splicing factor 3b subunit 1 (Sf3b1) haploinsufficient mice display features of low risk Myelodysplastic syndromes with ring sideroblasts
Authors: Valeria Visconte
Ali Tabarroki
Li Zhang
Yvonne Parker
Edy Hasrouni
Reda Mahfouz
Kyoichi Isono
Haruhiko Koseki
Mikkael A Sekeres
Yogen Saunthararajah
John Barnard
Daniel Lindner
Heesun J Rogers
Ramon V Tiu
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: Journal of Hematology & Oncology / Issue 1/2014
Electronic ISSN: 1756-8722
DOI: https://doi.org/10.1186/s13045-014-0089-x

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Abstract

Background

Methods

Results

Conclusions

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