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Open Access 01-12-2016 | Research Article

Molecular diagnosis and phylogenetic analysis of human papillomavirus type-16 from suspected patients in Pakistan

Authors: Abdullah Abdullah, Muhammad Qasim, Muhammad Shafiq, Muhammad Ijaz, Shamsa Parveen, Shahnaz Murtaza, Qamar Javed, Salman Akbar Malik, Shahida Husain Tarar, Sajid Mehmood, Abdul Sami, Syed Muhammad Saqlan Naqvi, Muhammad Zeeshan Hyder

Published in: Infectious Agents and Cancer | Issue 1/2016

Abstract

Background

Human Papillomavirus (HPV) is well known pathogen that can cause benign and malignant tumors in humans, yet there is very little information regarding HPV types prevalent in Pakistan.

Methods

A total of 92 cervical secretions were collected from suspected married female patients and used for DNA isolation using a novel isolation method. The samples were tested through Polymerase Chain Reaction (PCR) using already reported primers MY09/MY11, GP5/GP6, GP5+/GP6+, CP65/CP70, CP66/CP69 and SPF1/SPF2 and with those developed in this study including HRT1 and HRT2 primer sets for typing HPV types and HACTB primer set for human beta actin gene as internal positive control. Sequencing and phylogenetic analyses were performed for two isolates to determine circulating HPV types.

Results

PCR with HRT1 and HRT2 indicated 2 (2.17 %) patients were positive for HPV type- 16 while 1 (1.08 %) with HPV type 18. Sequencing and phylogenetic analysis of isolates confirmed HPV type-16 in genus alpha 9 which have 99 % homology with already reported HPV from Japan and Costa Rica.

Conclusion

This is the first report of HPV type-16 genus alpha 9 in Pakistan and the reported assay and sequence data will serve as valuable tools in further epidemiological studies for HPV surveillance to improve public health, especially of females in Pakistan.

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Title: Molecular diagnosis and phylogenetic analysis of human papillomavirus type-16 from suspected patients in Pakistan
Authors: Abdullah Abdullah
Muhammad Qasim
Muhammad Shafiq
Muhammad Ijaz
Shamsa Parveen
Shahnaz Murtaza
Qamar Javed
Salman Akbar Malik
Shahida Husain Tarar
Sajid Mehmood
Abdul Sami
Syed Muhammad Saqlan Naqvi
Muhammad Zeeshan Hyder
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: Infectious Agents and Cancer / Issue 1/2016
Electronic ISSN: 1750-9378
DOI: https://doi.org/10.1186/s13027-016-0047-z

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Abstract

Background

Methods

Results

Conclusion

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