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Infectious Agents and Cancer
Published in: Infectious Agents and Cancer 1/2015

Open Access 01-12-2015 | Research Article

Genetic variability in E6 and E7 oncogenes of human papillomavirus Type 16 from Congolese cervical cancer isolates

Authors: Luc Magloire Anicet Boumba, Samira Zoa Assoumou, Lahoucine Hilali, Jean Victor Mambou, Donatien Moukassa, Mustapha Moulay Ennaji

Published in: Infectious Agents and Cancer | Issue 1/2015

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Abstract

Background

The molecular epidemiological studies showed that some variants of HPV-16, distributed geographically, would present a higher risk of causing cervical cancer. This study aimed to analyze nucleotide changes of HPV-16 E6 and E7 genomic regions from infected Southwestern Congolese women.

Methods

DNA of twenty HPV-16 isolates was analyzed by amplifying the E6 and E7 genes using type-specific primers PCR and direct sequencing. The sequences obtained were aligned with the HPV-16 GenBank reference sequences.

Results

Thirteen (65.0%) out of 20 DNA-samples were successfully amplified. Genetic analysis revealed 18 and 4 nucleotide changes in E6 and E7 genomic regions respectively. The most frequently observed nucleotide variations were the missense C143G, G145T and C335T in E6 (100%), leading to the non-synonymous amino acid variation Q14D and H78Y. E7 genomic region was found to be highly conserved with two most common T789C and T795G (100%) silent variations. All HPV-16 variants identified belonged to the African lineage: 7 (53.8%) belonged to Af-1 lineage and 6 (46.1%) to Af-2 lineage. The missense mutation G622A (D21N) in the E7 region seems to be described for the first time in this study.

Conclusion

This study reported for the first time the distribution of HPV-16 E6 and E7 genetic variants in infected women from southwest Congo. The findings confirmed almost ascendancy of the African lineage in our study population.
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Metadata
Title
Genetic variability in E6 and E7 oncogenes of human papillomavirus Type 16 from Congolese cervical cancer isolates
Authors
Luc Magloire Anicet Boumba
Samira Zoa Assoumou
Lahoucine Hilali
Jean Victor Mambou
Donatien Moukassa
Mustapha Moulay Ennaji
Publication date
01-12-2015
Publisher
BioMed Central
Published in
Infectious Agents and Cancer / Issue 1/2015
Electronic ISSN: 1750-9378
DOI
https://doi.org/10.1186/s13027-015-0010-4

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