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Open Access 01-12-2019 | Research

Metabolome and transcriptome analyses reveal quality change in the orange-rooted Salvia miltiorrhiza (Danshen) from cultivated field

Authors: Zhilai Zhan, Wentao Fang, Xiaohui Ma, Tong Chen, Guanghong Cui, Ying Ma, Liping Kang, Tiegui Nan, Huixin Lin, Jinfu Tang, Yan Zhang, Changjiangsheng Lai, Zhenli Ren, Yanan Wang, Yujun Zhao, Ye Shen, Ling Wang, Wen Zeng, Juan Guo, Luqi Huang

Published in: Chinese Medicine | Issue 1/2019

Abstract

Background

The dry root and rhizome of Salvia miltiorrhiza Bunge, or Danshen, is a well-known, traditional Chinese medicine. Tanshinones are active compounds that accumulate in the periderm, resulting in red-colored roots. However, lines with orange roots have been observed in cultivated fields. Here, we performed metabolome and transcriptome analyses to investigate the changes of orange-rooted Danshen.

Methods

Metabolome analysis was performed by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-Tof–MS) to investigate the metabolites variation between orange Danshen and normal Danshen. RNA sequencing and KEGG enrichment analysis were performed to analyzing the differentially expressed genes between orange-rooted and normal Danshen.

Results

In total, 40 lipophilic components were detected in metabolome analysis, and seven compounds were significantly decreased in the orange Danshen, including the most abundant active compounds, tanshinone IIA and tanshinone I in normal Danshen. Systematic analysis of transcriptome profiles revealed that the down-regulated genes related to catalytic dehydrogenation was not detected. However, two genes related to stress resistance, and four genes related to endoplasmic reticulum (ER)-associated degradation of proteins were up-regulated in orange Danshen.

Conclusions

Decreases in the content of dehydrogenated furan ring tanshinones such as tanshinone IIA resulted in phenotypic changes and quality degradation of Danshen. Transcriptome analysis indicated that incorrect folding and ER-associated degradation of corresponding enzymes, which could catalyze C₁₅-C₁₆ dehydrogenase, might be contributed to the decrease in dehydrogenated furan ring tanshinones, rather than lower expression of the relative genes. This limited dehydrogenation of cryptotanshinone and dihydrotanshinone I into tanshinones IIA and I products, respectively, led to a reduced quality of Danshen in cultivated fields.

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Title: Metabolome and transcriptome analyses reveal quality change in the orange-rooted Salvia miltiorrhiza (Danshen) from cultivated field
Authors: Zhilai Zhan
Wentao Fang
Xiaohui Ma
Tong Chen
Guanghong Cui
Ying Ma
Liping Kang
Tiegui Nan
Huixin Lin
Jinfu Tang
Yan Zhang
Changjiangsheng Lai
Zhenli Ren
Yanan Wang
Yujun Zhao
Ye Shen
Ling Wang
Wen Zeng
Juan Guo
Luqi Huang
Publication date: 01-12-2019
Publisher: BioMed Central
Published in: Chinese Medicine / Issue 1/2019
Electronic ISSN: 1749-8546
DOI: https://doi.org/10.1186/s13020-019-0265-6

2024 ASCO Annual Meeting

Springer Medicine

Metabolome and transcriptome analyses reveal quality change in the orange-rooted Salvia miltiorrhiza (Danshen) from cultivated field

Abstract

Background

Methods

Results

Conclusions

2024 ASCO Annual Meeting

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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