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Journal of Translational Medicine
Published in: Journal of Translational Medicine 1/2017

Open Access 01-12-2017 | Research

Cytokine and autoantibody clusters interaction in systemic lupus erythematosus

Authors: Yovana Pacheco, Julián Barahona-Correa, Diana M. Monsalve, Yeny Acosta-Ampudia, Manuel Rojas, Yhojan Rodríguez, Juliana Saavedra, Mónica Rodríguez-Jiménez, Rubén D. Mantilla, Carolina Ramírez-Santana, Nicolás Molano-González, Juan-Manuel Anaya

Published in: Journal of Translational Medicine | Issue 1/2017

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Abstract

Background

Evidence supports the existence of different subphenotypes in systemic lupus erythematosus (SLE) and the pivotal role of cytokines and autoantibodies, which interact in a highly complex network. Thus, understanding how these complex nonlinear processes are connected and observed in real-life settings is a major challenge. Cluster approaches may assist in the identification of these subphenotypes, which represent such a phenomenon, and may contribute to the development of personalized medicine. Therefore, the relationship between autoantibody and cytokine clusters in SLE was analyzed.

Methods

This was an exploratory study in which 67 consecutive women with established SLE were assessed. Clinical characteristics including disease activity, a 14-autoantibody profile, and a panel of 15 serum cytokines were measured simultaneously. Mixed-cluster methodology and bivariate analyses were used to define autoantibody and cytokine clusters and to identify associations between them and related variables.

Results

First, three clusters of autoantibodies were defined: (1) neutral, (2) antiphospholipid antibodies (APLA)-dominant, and (3) anti-dsDNA/ENA-dominant. Second, eight cytokines showed levels above the threshold thus making possible to find 4 clusters: (1) neutral, (2) chemotactic, (3) G-CSF dominant, and (4) IFNα/Pro-inflammatory. Furthermore, the disease activity was associated with cytokine clusters, which, in turn, were associated with autoantibody clusters. Finally, when all biomarkers were included, three clusters were found: (1) neutral, (2) chemotactic/APLA, and (3) IFN/dsDNA, which were also associated with disease activity.

Conclusion

These results support the existence of three SLE cytokine-autoantibody driven subphenotypes. They encourage the practice of personalized medicine, and support proof-of-concept studies.
Appendix
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Metadata
Title
Cytokine and autoantibody clusters interaction in systemic lupus erythematosus
Authors
Yovana Pacheco
Julián Barahona-Correa
Diana M. Monsalve
Yeny Acosta-Ampudia
Manuel Rojas
Yhojan Rodríguez
Juliana Saavedra
Mónica Rodríguez-Jiménez
Rubén D. Mantilla
Carolina Ramírez-Santana
Nicolás Molano-González
Juan-Manuel Anaya
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Journal of Translational Medicine / Issue 1/2017
Electronic ISSN: 1479-5876
DOI
https://doi.org/10.1186/s12967-017-1345-y

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