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BMC Complementary Medicine and Therapies

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Open Access 01-12-2024 | Research

Potential role of Citrus bergamia flower essential oil against oral pathogens

Authors: Muhammad Imran Aziz, Muhammad Mohtasheemul Hasan, Riaz Ullah, Ahmed Bari, Mohsin Abbas Khan, Syed Zia Ul Hasnain, Rabia Baloch, Muhammad Akram, Aqsa obaid, Aziz Ullah, Khizar Abbas, Adnan Amin

Published in: BMC Complementary Medicine and Therapies | Issue 1/2024

Abstract

Background

Oral bacterial infections are difficult to treat due to emergence of resistance against antibiotic therapy. Essential oils are considered emerging alternate therapy against bacterial infections and biofilms. We investigated Citrus bergemia flower essential oil against oral pathogens.

Methods

The essential oil was analsyed using Gas Chromatography(GC–MS), in silico investigations, antioxidant, antimicrobial, antibiofilm and antiquorum sensing assays.

Results

Gas Chromatography analysis confirmed presence of 17 compounds including 1,6-Octadien-3-ol,3,7-dimethyl, 48.17%), l-limonene (22.03%) and p-menth-1-ol, 8-ol (7.31%) as major components. In silico analysis showed compliance of all tested major components with Lipinski’s rule, Bioavailability and antimicrobial activity using PASS (prediction of activity spectrum of substances). Molecular docking with transcriptional regulators 3QP5, 5OE3, 4B2O and 3Q3D revealed strong interaction of all tested compounds except 1,6-Octadien-3-ol,3,7-dimethyl. All tested compounds presented significant inhibition of DPPH (2,2-diphenyl-1-picrylhydrazyl) (IC₅₀ 0.65 mg/mL), H₂O₂ (hydrogen peroxide) (63.5%) and high FRAP (ferrous reducing antioxidant power) value (239.01 µg). In antimicrobial screening a significant activity (MIC 0.125 mg/mL) against Bacillus paramycoides and Bacillus chungangensis was observed. Likewise a strong antibiofilm (52.1 – 69.5%) and anti-QS (quorum sensing) (4–16 mm) activity was recorded in a dose dependent manner.

Conclusion

It was therefore concluded that C. bergemia essential oil posess strong antioxidant, antimicrobial and antibiofilm activities against tested oral pathogens.

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Title: Potential role of Citrus bergamia flower essential oil against oral pathogens
Authors: Muhammad Imran Aziz
Muhammad Mohtasheemul Hasan
Riaz Ullah
Ahmed Bari
Mohsin Abbas Khan
Syed Zia Ul Hasnain
Rabia Baloch
Muhammad Akram
Aqsa obaid
Aziz Ullah
Khizar Abbas
Adnan Amin
Publication date: 01-12-2024
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2024
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-024-04457-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Potential role of Citrus bergamia flower essential oil against oral pathogens

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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