Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2017

Open Access 01-12-2017 | Research article

Anti-inflammatory and analgesic activities of solvent fractions of the leaves of Moringa stenopetala Bak. (Moringaceae) in mice models

Authors: Yohannes Tamrat, Teshome Nedi, Solomon Assefa, Tilahun Teklehaymanot, Workineh Shibeshi

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

Login to get access

Abstract

Background

Many people still experience pain and inflammation regardless of the available drugs for treatments. In addition, the available drugs have many side effects, which necessitated a quest for new drugs from several sources in which medicinal plants are the major one. This study evaluated the analgesic and anti- inflammatory activity of the solvent fractions of Moringa stenopetala in rodent models of pain and inflammation.

Methods

Successive soxhlet and maceration were used as methods of extractions using solvents of increasing polarity; chloroform, methanol and water. Swiss albino mice models were used in radiant tail flick latency, acetic acid induced writhing and carrageenan induced paw edema to assess the analgesic and anti-inflammatory activities. The test groups received different doses (100 mg/kg, 200 mg/kg and 400 mg/kg) of the three fractions (chloroform, methanol and aqueous). The positive control groups received morphine (20 mg/kg) or aspirin (100 mg/kg or 150 mg/kg) based on the respective models. The negative control groups received the 10 ml/kg of vehicles (distilled water or 2% Tween 80).

Results

In all models, the chloroform fraction had protections only at a dose of 400 mg/kg. However, the methanol and aqueous fraction at all doses have shown significant central and peripheral analgesic activities with a comparable result to the standards. The aqueous and methanol fractions significantly reduced carrageenan induced inflammation in a dose dependent manner, in which the highest reduction of inflammation was observed in aqueous fraction at 400 mg/kg.

Conclusion

This study provided evidence on the traditionally claimed uses of the plant in pain and inflammatory diseases, and Moringa stenopetala could be potential source for development of new analgesic and anti-inflammatory drugs.
Literature
1.
2.
Rajagopal M. Pain - Basic considerations. Indian J Anaesth. 2006;50(5):331–4.
3.
Debon D, Hoeksema L, Hobbs R. Caring for Patients with Chronic Pain: Pearls and Pitfalls. J Am Osteopath Assoc. 2013;113:620–7.CrossRef
4.
Breivik H, Eisenberg E, O’Brien T. The indivvidual and societal burden of chronic pain in Europe: The case for strategic prioritization and action to improve knowledge and availability of appropriate care. BMC Public Health. 2013;13(1229):1–14.
5.
Underwood J. General and Systematic Pathology. 4th ed. Edinburgh: Churchill Livingstone; 2004. p. 201–23.
6.
Kumar R, Clermont G, Vodovotz Y, et al. The dynamics of acute inflammation. J Theor Biol. 2004;230:145–55.CrossRefPubMed
7.
Shojaii A, Motaghinejad M, Norouzi S, Motevalian M. Evaluation of Anti -inflammatory and Analgesic Activity of the Extract and Fractions of Astragalus hamosus in Animal Models. Iran J Pharm Res. 2015;14(1):263–9.PubMedPubMedCentral
8.
9.
Nash R, Yates P, Edwards H, et al. Pain and administration of analgesia: what nurses say? J Clin Nurs. 1999;8(2):180–9.CrossRefPubMed
10.
Köksal M, Gökhan N, Küpeli E et al. Analgesic and Antiinflammatory Activities of Some New Mannich Bases of 5-Nitro-2-Benzoxazolinones. Arch Pharm Res 2007; 30(4): 419-424.
11.
Munir F, Yarker J, Haslam C. Use of prescribed medication at work in employees with chronic illness. Occup Med. 2007;57:480–7.CrossRef
12.
Niemi T, Taxell C, Rosenberg P. Comparison of the effect of intravenous ketoprofen, ketorolac and diclofenac on platelet function in volunteers. Acta Anaesthesiol Scand. 1997;41:1353–8.CrossRefPubMed
13.
Mital R, Edwin C, Suresh B. Investigation of centrally and peripherally acting analgesic and anti-inflammatory activity of biological immune response modulator (an Amazonian plant extract) in animal models of pain and inflammation. Int J Basic Clin Pharmacol. 2015;4:342–8.
14.
Vittarao A, Shanbhag T, Kumari K, et al. Evaluation of anti-inflammatory and analgesic of alcoholic extract of Kaemperia Galagna in rats. Indian J Physiol Pharmacol. 2011;55(1):13–24.
15.
Bekele E: Study on Actual Situation of Medicinal Plants in Ethiopia. Prepared for Japan association for international Collaboration of Agriculture and Forestry, 2007; Addis Ababa.
16.
Mesfin K, Tekle G, Tesfay T. Ethnobotanical study of traditional medicinal plants used by indigenous people of Gemad District, Northern Ethiopia. J Med Plants Stud. 2013;1(4):32–7.
17.
Giday M. An ethnobotanical study of medicinal plants used by the Zay people in Ethiopia. CBMs Skritserie. 2001;3:81–99.
18.
Yirga G. Ethnobotanical study of medicinal plants in and around Alamata, Southern Tigray, Northern Ethiopia. Curr Res J Biol Sci. 2010;2(5):338–44.
19.
Bekele G, Reddy P. Ethnobotanical study of medicinal plants used to treat human ailments by Guji Oromo tribes in Abaya District, Borana, Oromia, Ethiopia. Univ J Plant Sci. 2015;3(1):1–8.
20.
Teklehaymanot T, Giday M. Ethnobotanical study of wild edible plants of Kara and Kwego semi-pastoralist people in Lower Omo River Valley, Debub Omo Zone, SNNPR, Ethiopia. J Ethno Biol Ethno Med. 2010;6(23):1–8.
21.
Jiru D, Sonder K, Alemayehu L, Mekonen Y, and Anjulo A. Leaf yield and Nutritive value of Moringa stenopetala and Moringa oleifera Accessions: Its potential role in food security in constrained dry farming agroforestry system. Moringa and other highly nutritious plant resources: Strategies, standards and markets for a better impact on nutrition in Africa. Accra, Ghana; 2006: p. 1–14.
22.
Ghebreselassie D, Mekonnen Y, Gebru G, Ergete W, Huruy K. The effects of Moringa stenopetala on blood parameters and histopathology of liver and kidney in mice. Ethiop J Health Dev. 2011;25(1):51–7.CrossRef
23.
Mekonnen Y, Amare G, Ermias D, et al. The multi-purpose Moringa tree: Ethiopia Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia. 1996;10:111-118.
24.
Musa A, Vata P, Debella A. Acute toxicity studies of butanol fraction of leaves of Moringa stenopetala in rats. Asian Pac J Health Sci. 2015;2(2):160–4.
25.
Geremew H, Shibeshi W, Engidawork E, Tamiru W. Evaluation of Analgesic and Anti-inflammatory activity of Moringa stenopetala Bak. (Moringaceae) in mice. Ethiop J Pharmaceut. 2015;31:15–26.CrossRef
26.
Negus S, Vanderah TW, Brandt MR, Bilsky EJ, Becerra L, Borsook D. Preclinical assessment of candidate analgesic drugs: recent advances and future challenges. J Pharmacol Exp Ther. 2006;319(2):507–14.CrossRefPubMed
27.
Institute for Laboratory Animal Research (ILAR): Guide for the care and use of laboratory animals. Washington DC: National Academy Press. 1996.
28.
Ayoola G, Coker H, Adesegun S, Adepoju-Bello A, Obaweya K, Ezennia E, Atangbayila T. Phytochemical screening and antioxidant activities of some selected medicinal Plants used for malaria therapy in Southwestern Nigeria. Trop J Pharm Res. 2008;7(3):1019–24.
29.
Sasidharan S, ChenY SD, et al. Extraction, isolation and characterization of bioactive compounds from plants’ extracts. Afr J Tradit Complement Altern Med. 2011;8:1–10.PubMed
30.
OECD: Guidelines for Testing of Chemicals: Guideline 425: Acute Oral Toxicity. Paris, France. The Organization of Economic Co-operation and Development.2008.
31.
D'amour F, Smith D. A method for determining loss of pain sensation. J Pharmacol Exp Ther. 1941;72(1):74–9.
32.
Milind P, Monu Y. Laboratory models for screening analgesics. Int Res J Pharm. 2013;4:15–9.
33.
Torres IL, Vasconcellos AP, Cucco SNS, et al. Effect of repeated stress on novelity induced anti-nociception in rats. Braz J Med Biol Res. 2001;34:241–4.CrossRefPubMed
34.
Koster R, Anderson M, De Beer E. Acetic acid analgesic screening. Fed Proc. 1959;18:412–6.
35.
Hernández-Ortega M, Ortiz-Moreno A, Hernández-Navarro M, Chamorro-Cevallos G, Dorantes-Alvarez L, et al. Antioxidant, antinociceptive and anti-inflammatory effects of Carotenoids extracted from dried pepper (Capsicum annuum L). J Biomed Biotechnol. 2012:1-10.
36.
Winter C, Risley E, And Nuss C. Carrageenan-induced oedema in the hind paw of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp Biol Med. 1962;111:544–7.CrossRefPubMed
37.
Olukunle JO, Adenubi OT, Oladele GM, et al. Studies on the anti-inflammatory and analgesic properties of Jatropha curcas leaf extract. Acta Vet Brno. 2011;80:259–62.CrossRef
38.
WHO. Hazard classification-acute LD50 values of formulated products. In: The guidebook to the registration of public health pesticides and repellents against Vectors.1975.
39.
40.
Vegeto E, Benedusi V, Maggi A. Estrogen anti-inflammatory activity in brain: A therapeutic opportunity for menopause and neurodegenerative diseases. Front Neuroendocrinol. 2008;29(4):1–29.CrossRef
41.
Siddalingappa C, Rajesh T, Kudagi B. Evaluation of analgesic and anti-inflammatory activites of Tinospora cordifolia in rodents. Int J Basic Med Sci. 2012;2(6):306–11.
42.
Silva J, Abebe W, Sonsa SM, et al. Analgesic and antiinflammatory effects of essential oil of Eucalyptus. J Ethnopharmacol. 2003;89:277–83.CrossRefPubMed
43.
Marzouk B, Marzouk Z, Fenina N, et al. Anti-inflammatory and analgesic activities of Tunisian Citrullus colocynthis Schrad, Immature fruit and seed organic extracts. Eur Rev Med Pharmacol Sci. 2011;15:665–72.PubMed
44.
Le Bars D, Gozariu M, Cadden SW. Animal models of nociception. Pharmacol Rev. 2001;53(4):597–652.PubMed
45.
Mandegary A, Sayyah M, Heidari MR. Antinociceptive and anti-inflammatory activity of the seed and root extracts of Ferula gummosa Boiss in mice and rats. Daru. 2004;12:58–62.
46.
Collier H, Dinneen L, Johnson C, Schneide C. Abdominal constriction response and its suppression by analgesic drugs in mouse. Br J Pharmacol. 1868;32:295–310.
47.
Satyanarayana P, Jain N, Singh S, Kulkarni S. Effect of selective inhibition of cyclooxygenase-2 on lipopolysaccharide induced hyperalgesia. Inflammopharmacol. 2004;12:57–68.CrossRef
48.
Awad A, Toczek J, Fink C. Phytosterols decrease prostaglandin release in cultured P388D1/MAB macrophages. Prostag Leukot Essent Fatty Acids. 2004;70(6):511–20.CrossRef
49.
Reanmongkol W, Subhadhirasakul S, Thienmontree S, Thanyapanit K, Kalnaowakul J, Sengsui S. Anti-nociceptive activity of the alkaloid extracts from Kopsia macrophylla leaves in mice. J Sci Technol. 2005;27:509–16.
50.
Woldesellassie M, Eyasu M, Kelbessa U. In vivo anti-inflammatory activities of leaf extracts of Ocimum lamiifolium in mice model. J Ethnopharmacol. 2011;134:32–6.CrossRef
51.
Carey W, Rao V, Kumar R, Mohan K. Anti-inflammatory and analgesic activities of methanolic extract of Kigelia pinnata DC flower. J Ethnopharmacol. 2010;130:179–82.CrossRef
52.
Juhas S, Bujnakova D, Rehak P, Ciko S, Czikkova S, Vesela J, Iikova G, Koppel J. Anti-Inflammatory effects of thyme essential oil in mice. Acta Vet Brno. 2008;77:327–34.CrossRef
53.
Yang R, Tsou S, Lee T. Moringa: a Novel Plant Rich in Antioxidants, Bioavailable Iron, and Nutrients. In: Wang M, Sang S, Hwang LS, Ho CT, editors. Herbs: Challenges in Chemistry and Biology; 2006. p. 224–39.CrossRef
54.
Çadirci E, Suleyman H, Gurbuz P, Kuruuzum A, Güvenalp Z, Demirezer L. Anti-infl ammatory effects of different extracts from three Salvia species. Turk J Biol. 36:59–64.
55.
Souto A, Tavares J, da Silva M. Anti-Inflammatory Activity of Alkaloids: An Update from 2000 to 2010. Molecules. 2011;6:8515–34.CrossRef
56.
Hosseinzadeh H, Younesi H. Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice. BMC Pharmacol. 2002;2(7):1–8.
Metadata
Title
Anti-inflammatory and analgesic activities of solvent fractions of the leaves of Moringa stenopetala Bak. (Moringaceae) in mice models
Authors
Yohannes Tamrat
Teshome Nedi
Solomon Assefa
Tilahun Teklehaymanot
Workineh Shibeshi
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-017-1982-y

Other articles of this Issue 1/2017

BMC Complementary Medicine and Therapies 1/2017 Go to the issue

Research article

Increase of glutathione, testosterone and antioxidant effects of Jurenia dolomiaea on CCl4 induced testicular toxicity in rat

Research article

Modified lingguizhugan decoction incorporated with dietary restriction and exercise ameliorates hyperglycemia, hyperlipidemia and hypertension in a rat model of the metabolic syndrome

Study protocol

Acupuncture for acute moderate thalamic hemorrhage: randomized controlled trial study protocol

Research article

A national survey of Chinese medicine doctors and clinical practice guidelines in China

Research article

The education of traditional Japanese (Kampo) medicine: surveys of training hospitals and residents

Study protocol

Wrist-ankle acupuncture (WAA) for primary dysmenorrhea (PD) of young females: study protocol for a randomized controlled trial