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Open Access 01-12-2023 | Cervical Cancer | Research

Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells

Authors: Fang Wang, Shuyan Song, Bingxuan Guo, Yangyang Li, Huijuan Wang, Shaowei Fu, Luyue Wang, Xiangyi Zhe, Hongtao Li, Dongmei Li, Renfu Shao, Zemin Pan

Published in: BMC Cancer | Issue 1/2023

Abstract

Background

Cervical cancer is a common gynecological malignancy. Gene microarray found that TCP11 gene was highly expressed in cervical cancer. However, the effect of TCP11 gene on the proliferation, apoptosis and migration of cervical cancer cells and its underlying molecular mechanisms are unclear.

Methods

GEPIA database, tissue microarray, western blot and qRT-PCR were used to analyze the expression of TCP11 gene in cervical cancer tissues and cells and its relationship with patients’ survival rate. The cell cycle and apoptosis were detected by flow cytometry, and the expressions of cell cycle and apoptosis related molecules and EMT-related molecules were detected by Western blot and qRT-PCR.

Results

The results showed that TCP11 gene was highly expressed in cervical cancer tissues and cells compared with normal cervical tissues and cells, and its expression was positively correlated with patients’ survival rate. The results of proliferation and migration assays showed that TCP11 overexpression inhibited the proliferation and migration of HeLa and SiHa cells. The results showed that TCP11 overexpression blocked the cell cycle of HeLa and SiHa cells, decreased the expression of CDK1 and Cyclin B1, and increased the apoptosis and the expression of caspase-3, cleaved-caspase-3 and cleaved-PARP. TCP11 overexpression increased the protein and mRNA expression of EMT-related molecules ZO-1 and E-cadherin. Conversely, TCP11 knockdown promoted the proliferation of HeLa and SiHa cells and the migration of HeLa cells.

Conclusions

TCP11 overexpression significantly inhibited the occurrence and development of cervical cancer cells, it may be a potentially beneficial biomarker for cervical cancer.

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Title: Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells
Authors: Fang Wang
Shuyan Song
Bingxuan Guo
Yangyang Li
Huijuan Wang
Shaowei Fu
Luyue Wang
Xiangyi Zhe
Hongtao Li
Dongmei Li
Renfu Shao
Zemin Pan
Publication date: 01-12-2023
Publisher: BioMed Central
Keywords: Cervical Cancer
Cervical Cancer
Published in: BMC Cancer / Issue 1/2023
Electronic ISSN: 1471-2407
DOI: https://doi.org/10.1186/s12885-023-11129-1

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