Top

Published in:

Open Access 01-12-2016 | Research article

Interleukin-22 is elevated in lavage from patients with lung cancer and other pulmonary diseases

Authors: Amanda Tufman, Rudolf Maria Huber, Stefanie Völk, Frederic Aigner, Martin Edelmann, Fernando Gamarra, Rosemarie Kiefl, Kathrin Kahnert, Fei Tian, Anne-Laure Boulesteix, Stefan Endres, Sebastian Kobold

Published in: BMC Cancer | Issue 1/2016

Abstract

Background

Interleukin-22 (IL-22) is involved in lung diseases such as pneumonia, asthma and lung cancer. Lavage mirrors the local environment, and may provide insights into the presence and role of IL-22 in patients.

Methods

Bronchoscopic lavage (BL) samples (n = 195, including bronchoalveolar lavage and bronchial washings) were analysed for IL-22 using an enzyme-linked immunosorbent assay. Clinical characteristics and parameters from lavage and serum were correlated with lavage IL-22 concentrations.

Results

IL-22 was higher in lavage from patients with lung disease than in controls (38.0 vs 15.3 pg/ml, p < 0.001). Patients with pneumonia and lung cancer had the highest concentrations (48.9 and 33.0 pg/ml, p = 0.009 and p < 0.001, respectively). IL-22 concentration did not correlate with systemic inflammation. IL-22 concentrations did not relate to any of the analysed cell types in BL indicating a potential mixed contribution of different cell populations to IL-22 production.

Conclusions

Lavage IL-22 concentrations are high in patients with lung cancer but do not correlate with systemic inflammation, thus suggesting that lavage IL-22 may be related to the underlying malignancy. Our results suggest that lavage may represent a distinct compartment where the role of IL-22 in thoracic malignancies can be studied.

Vivier E, Spits H, Cupedo T. Interleukin-22-producing innate immune cells: new players in mucosal immunity and tissue repair? Nat Rev Immunol. 2009;9(4):229–34.CrossRefPubMed

Whittington HA, Armstrong L, Uppington KM, Millar AB. Interleukin-22: a potential immunomodulatory molecule in the lung. Am J Respir Cell Mol Biol. 2004;31(2):220–6.CrossRefPubMed

Lilly LM, Gessner MA, Dunaway CW, et al. The beta-glucan receptor dectin-1 promotes lung immunopathology during fungal allergy via IL-22. J Immunol. 2012;189(7):3653–60.CrossRefPubMedPubMedCentral

Gessner MA, Werner JL, Lilly LM, et al. Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus. Infect Immun. 2012;80(1):410–7.CrossRefPubMedPubMedCentral

Simonian PL, Wehrmann F, Roark CL, et al. Gammadelta T cells protect against lung fibrosis via IL-22. J Exp Med. 2010;207(10):2239–53.CrossRefPubMedPubMedCentral

Huai JP, Sun XC, Chen MJ, et al. Melatonin attenuates acute pancreatitis-associated lung injury in rats by modulating interleukin 22. World J Gastroenterol. 2012;18(36):5122–8.CrossRefPubMedPubMedCentral

Hoegl S, Bachmann M, Scheiermann P, et al. Protective properties of inhaled IL-22 in a model of ventilator-induced lung injury. Am J Respir Cell Mol Biol. 2011;44(3):369–76.CrossRefPubMed

Aujla SJ, Chan YR, Zheng M, et al. IL-22 mediates mucosal host defense against Gram-negative bacterial pneumonia. Nat Med. 2008;14(3):275–81.CrossRefPubMedPubMedCentral

Besnard AG, Sabat R, Dumoutier L, et al. Dual role of IL-22 in allergic airway inflammation and its cross-talk with IL-17A. Am J Respir Crit Care Med. 2011;183(9):1153–63.CrossRefPubMed

10.

Paats MS, Bergen IM, Hanselaar WE, et al. Local and systemic cytokine profiles in non-severe and severe community-acquired pneumonia. Eur Respir J. 2013;41(6):1378–85.CrossRefPubMed

11.

Zhang W, Chen Y, Wei H, et al. Antiapoptotic activity of autocrine interleukin-22 and therapeutic effects of interleukin-22-small interfering RNA on human lung cancer xenografts. Clin Cancer Res. 2008;14(20):6432–9.CrossRefPubMed

12.

Kobold S, Volk S, Clauditz T, et al. Interleukin-22 is frequently expressed in small- and large-cell lung cancer and promotes growth in chemotherapy-resistant cancer cells. J Thorac Oncol. 2013;8(8):1032–42.CrossRefPubMed

13.

Baughman RP. Technical aspects of bronchoalveolar lavage: recommendations for a standard procedure. Semin Respir Crit Care Med. 2007;28(5):475–85.CrossRefPubMed

14.

Meyer KC, Raghu G. Bronchoalveolar lavage for the evaluation of interstitial lung disease: is it clinically useful? Eur Respir J. 2011;38(4):761–9.CrossRefPubMed

15.

Meyer KC. Bronchoalveolar lavage as a diagnostic tool. Semin Respir Crit Care Med. 2007;28(5):546–60.CrossRefPubMed

16.

Hoffken G, Lorenz J, Kern W, et al. Epidemiology, diagnosis, antimicrobial therapy and management of community-acquired pneumonia and lower respiratory tract infections in adults. Guidelines of the Paul-Ehrlich-Society for Chemotherapy, the German Respiratory Society, the German Society for Infectiology and the Competence Network CAPNETZ Germany. Pneumologie. 2009;63(10):e1–68.CrossRefPubMed

17.

Lachenbruch PA. Comparisons of two-part models with competitors. Stat Med. 2001;20(8):1215–34.CrossRefPubMed

18.

Huber S, Gagliani N, Zenewicz LA, et al. IL-22BP is regulated by the inflammasome and modulates tumorigenesis in the intestine. Nature. 2012;491(7423):259–63.CrossRefPubMedPubMedCentral

19.

Pennino D, Bhavsar PK, Effner R, et al. IL-22 suppresses IFN-gamma-mediated lung inflammation in asthmatic patients. J Allergy Clin Immunol. 2013;131(2):562–70.CrossRefPubMed

20.

Yao S, Huang D, Chen CY, et al. Differentiation, distribution and gammadelta T cell-driven regulation of IL-22-producing T cells in tuberculosis. PLoS Pathog. 2010;6:2.CrossRef

21.

Paats MS, Bergen IM, Hanselaar WE, et al. T helper 17 cells are involved in the local and systemic inflammatory response in community-acquired pneumonia. Thorax. 2013;68(5):468–74.CrossRefPubMed

22.

Scriba TJ, Kalsdorf B, Abrahams DA, et al. Distinct, specific IL-17- and IL-22-producing CD4+ T cell subsets contribute to the human anti-mycobacterial immune response. J Immunol. 2008;180(3):1962–70.CrossRefPubMedPubMedCentral

23.

Ten Berge B, Paats MS, Bergen IM, et al. Increased IL-17A expression in granulomas and in circulating memory T cells in sarcoidosis. Rheumatology. 2012;51(1):37–46.CrossRefPubMed

24.

Liu T et al. Increased circulating Th22 and Th17 cells are associated with tumor progression and patient survival in human gastric cancer. J Clin Immunol. 2012;32(6):1332–9.CrossRefPubMed

25.

Kuang DM et al. B7-H1-expressing antigen-presenting cells mediate polarization of protumorigenic Th22 subsets. J Clin Invest. 2014;124(10):4657–67.CrossRefPubMedPubMedCentral

26.

Kryczek I et al. IL-22(+)CD4(+) T cells promote colorectal cancer stemness via STAT3 transcription factor activation and induction of the methyltransferase DOT1L. Immunity. 2014;40(5):772–84.CrossRefPubMedPubMedCentral

27.

Ye ZJ et al. Interleukin 22-producing CD4+ T cells in malignant pleural effusion. Cancer Lett. 2012;326(1):23–32.CrossRefPubMed

28.

Hollander C, Sitkauskiene B, Sakalauskas R, Westin U, Janciauskiene SM. Serum and bronchial lavage fluid concentrations of IL-8, SLPI, sCD14 and sICAM-1 in patients with COPD and asthma. Respir Med. 2007;101(9):947–53.CrossRef

29.

Schreiber G, McCrory DC. Performance characteristics of different modalities for diagnosis of suspected lung cancer: summary of published evidence. Chest. 2003;123(1 Suppl):115S–28.CrossRefPubMed

Title: Interleukin-22 is elevated in lavage from patients with lung cancer and other pulmonary diseases
Authors: Amanda Tufman
Rudolf Maria Huber
Stefanie Völk
Frederic Aigner
Martin Edelmann
Fernando Gamarra
Rosemarie Kiefl
Kathrin Kahnert
Fei Tian
Anne-Laure Boulesteix
Stefan Endres
Sebastian Kobold
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: BMC Cancer / Issue 1/2016
Electronic ISSN: 1471-2407
DOI: https://doi.org/10.1186/s12885-016-2471-2

Webinar | 19-02-2024 | 17:30 (CET)

Keynote webinar | Spotlight on antibody–drug conjugates in cancer

Watch now

Antibody–drug conjugates (ADCs) are novel agents that have shown promise across multiple tumor types. Explore the current landscape of ADCs in breast and lung cancer with our experts, and gain insights into the mechanism of action, key clinical trials data, existing challenges, and future directions.

Dr. Véronique Diéras

Prof. Fabrice Barlesi

Developed by: Springer Medicine

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 1/2016

Prognostic and predictive significance of long interspersed nucleotide element-1 methylation in advanced-stage colorectal cancer

Computerized tomography angiography in preoperative assessment of intravenous leiomyomatosis extending to inferior vena cava and heart

Collapsin response mediator protein 4 isoforms (CRMP4a and CRMP4b) have opposite effects on cell proliferation, migration, and invasion in gastric cancer

New anti-cancer chemicals Ertredin and its derivatives, regulate oxidative phosphorylation and glycolysis and suppress sphere formation in vitro and tumor growth in EGFRvIII-transformed cells

Methionine-restricted diet inhibits growth of MCF10AT1-derived mammary tumors by increasing cell cycle inhibitors in athymic nude mice

Fluorouracil implants caused a diaphragmatic tumor to be misdiagnosed as liver metastasis: a case report

Keynote webinar | Spotlight on antibody–drug conjugates in cancer