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Critical Care

01-06-2006 | Commentary

The struggle to detect circulating DNA

Author: Sacha Zeerleder

Published in: Critical Care | Issue 3/2006

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Abstract

In various diseases, such as cancer, autoimmune disease, sepsis or myocardial infarction, elevated levels of circulating DNA can be measured. However, its predictive value is under debate. Circulating DNA in plasma is protein-bound (nucleosomal) DNA. Quantification of circulating DNA can be performed by real-time quantitative PCR or immunological methods such as ELISA. The diagnostic value of both methods can be impaired by inappropriate handling of the samples. Assessment of circulating DNA in patients admitted to the intensive care unit offers a tool for predicting morbidity and mortality.
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Metadata
Title
The struggle to detect circulating DNA
Author
Sacha Zeerleder
Publication date
01-06-2006
Publisher
BioMed Central
Published in
Critical Care / Issue 3/2006
Electronic ISSN: 1364-8535
DOI
https://doi.org/10.1186/cc4932