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Breast Cancer Research
Published in: Breast Cancer Research 5/2007

Open Access 01-10-2007 | Research article

Gene expression profiling and histopathological characterization of triple-negative/basal-like breast carcinomas

Authors: Bas Kreike, Marieke van Kouwenhove, Hugo Horlings, Britta Weigelt, Hans Peterse, Harry Bartelink, Marc J van de Vijver

Published in: Breast Cancer Research | Issue 5/2007

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Abstract

Introduction

Breast cancer is a heterogeneous group of tumors, and can be subdivided on the basis of histopathological features, genetic alterations and gene-expression profiles. One well-defined subtype of breast cancer is characterized by a lack of HER2 gene amplification and estrogen and progesterone receptor expression ('triple-negative tumors'). We examined the histopathological and gene-expression profile of triple-negative tumors to define subgroups with specific characteristics, including risk of developing distant metastases.

Methods

97 triple-negative tumors were selected from the fresh-frozen tissue bank of the Netherlands Cancer Institute, and gene-expression profiles were generated using 35K oligonucleotide microarrays. In addition, histopathological and immunohistochemical characterization was performed, and the findings were associated to clinical features.

Results

All triple-negative tumors were classified as basal-like tumors on the basis of their overall gene-expression profile. Hierarchical cluster analysis revealed five distinct subgroups of triple-negative breast cancers. Multivariable analysis showed that a large amount of lymphocytic infiltrate (HR = 0.30, 95% CI 0.09–0.96) and absence of central fibrosis in the tumors (HR = 0.14, 95% CI 0.03–0.62) were associated with distant metastasis-free survival.

Conclusion

Triple-negative tumors are synonymous with basal-like tumors, and can be identified by immunohistochemistry. Based on gene-expression profiling, basal-like tumors are still heterogeneous and can be subdivided into at least five distinct subgroups. The development of distant metastasis in basal-like tumors is associated with the presence of central fibrosis and a small amount of lymphocytic infiltrate.
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Metadata
Title
Gene expression profiling and histopathological characterization of triple-negative/basal-like breast carcinomas
Authors
Bas Kreike
Marieke van Kouwenhove
Hugo Horlings
Britta Weigelt
Hans Peterse
Harry Bartelink
Marc J van de Vijver
Publication date
01-10-2007
Publisher
BioMed Central
Published in
Breast Cancer Research / Issue 5/2007
Electronic ISSN: 1465-542X
DOI
https://doi.org/10.1186/bcr1771

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