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Open Access 01-10-2005 | Research article

Early detection of breast cancer based on gene-expression patterns in peripheral blood cells

Authors: Praveen Sharma, Narinder S Sahni, Robert Tibshirani, Per Skaane, Petter Urdal, Hege Berghagen, Marianne Jensen, Lena Kristiansen, Cecilie Moen, Pradeep Sharma, Alia Zaka, Jarle Arnes, Torill Sauer, Lars A Akslen, Ellen Schlichting, Anne-Lise Børresen-Dale, Anders Lönneborg

Published in: Breast Cancer Research | Issue 5/2005

Abstract

Introduction

Existing methods to detect breast cancer in asymptomatic patients have limitations, and there is a need to develop more accurate and convenient methods. In this study, we investigated whether early detection of breast cancer is possible by analyzing gene-expression patterns in peripheral blood cells.

Methods

Using macroarrays and nearest-shrunken-centroid method, we analyzed the expression pattern of 1,368 genes in peripheral blood cells of 24 women with breast cancer and 32 women with no signs of this disease. The results were validated using a standard leave-one-out cross-validation approach.

Results

We identified a set of 37 genes that correctly predicted the diagnostic class in at least 82% of the samples. The majority of these genes had a decreased expression in samples from breast cancer patients, and predominantly encoded proteins implicated in ribosome production and translation control. In contrast, the expression of some defense-related genes was increased in samples from breast cancer patients.

Conclusion

The results show that a blood-based gene-expression test can be developed to detect breast cancer early in asymptomatic patients. Additional studies with a large sample size, from women both with and without the disease, are warranted to confirm or refute this finding.

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Title: Early detection of breast cancer based on gene-expression patterns in peripheral blood cells
Authors: Praveen Sharma
Narinder S Sahni
Robert Tibshirani
Per Skaane
Petter Urdal
Hege Berghagen
Marianne Jensen
Lena Kristiansen
Cecilie Moen
Pradeep Sharma
Alia Zaka
Jarle Arnes
Torill Sauer
Lars A Akslen
Ellen Schlichting
Anne-Lise Børresen-Dale
Anders Lönneborg
Publication date: 01-10-2005
Publisher: BioMed Central
Published in: Breast Cancer Research / Issue 5/2005
Electronic ISSN: 1465-542X
DOI: https://doi.org/10.1186/bcr1203

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Abstract

Introduction

Methods

Results

Conclusion

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