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European Journal of Medical Research

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01-12-2009 | Research

Macrolide antibiotics like azithromycin increase lipopolysaccharide-induced IL-8 production by human gingival fibroblasts

Authors: A Kamemoto, T Ara, T Hattori, Y Fujinami, Y Imamura, P-L Wang

Published in: European Journal of Medical Research | Issue 7/2009

Abstract

Objective

Macrolide antibiotics are reported to modulate the production of cytokines in various type of cells. We examined the effect of macrolide antibiotics on inflammatory cytokines (IL-6 and IL-8) and chemical mediator (PGE₂) and also matrix metalloproteinases (MMPs) productions by human gingival fibroblasts (HGFs) treated with lipopolysaccharide (LPS).

Methods

The effect of macrolide antibiotics [erythromycin (EM), azithromycin (AZM) and josamycin (JOM)] on HGFs proliferation were examined by MTT assay. HGFs were treated with LPS from Porphyromonas gingivalis (PgLPS) and macrolide antibiotics, and IL-6, IL-8 and PGE₂ levels were evaluated by ELISA. MMPs were detected by gelatin zymography.

Results

AZM slightly but significantly decreased HGFs proliferation, while EM and JOM did not affected. AZM increased PgLPS-induced IL-8 production dose-dependently, while AZM did not alter IL-6 and PGE₂ productions. EM and JOM did not altered PgLPS-induced IL-6, IL-8 and PGE₂ productions. All macrolide antibiotics did not alter MMPs production. These results indicate that macrolide antibiotics have no direct anti-inflammatory effect. However, the use of the inhibitors of cell signaling pathway failed to reveal the mechanism that AZM enhanced PgLPS-induced IL-8 production.

Conclusion

These results suggest macrolide antibiotics have an indirect anti-inflammatory effect as a result of their antimicrobial properties. Because AZM increased LPS-induced IL-8 production by HGFs, the possibility is considered that neutrophils may be migrated to periodontal tissue and phagocytize the periodontopathic bacteria more efficiently.

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Title: Macrolide antibiotics like azithromycin increase lipopolysaccharide-induced IL-8 production by human gingival fibroblasts
Authors: A Kamemoto
T Ara
T Hattori
Y Fujinami
Y Imamura
P-L Wang
Publication date: 01-12-2009
Publisher: BioMed Central
Published in: European Journal of Medical Research / Issue 7/2009
Electronic ISSN: 2047-783X
DOI: https://doi.org/10.1186/2047-783X-14-7-309

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Macrolide antibiotics like azithromycin increase lipopolysaccharide-induced IL-8 production by human gingival fibroblasts

Abstract

Objective

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Methods

Results

Conclusion

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