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Medical Gas Research
Published in: Medical Gas Research 1/2012

Open Access 01-12-2012 | Study protocol

A convenient method for determining the concentration of hydrogen in water: use of methylene blue with colloidal platinum

Authors: Tomoki Seo, Ryosuke Kurokawa, Bunpei Sato

Published in: Medical Gas Research | Issue 1/2012

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Abstract

A simple titration (oxidimetry) method using a methylene blue-platinum colloid reagent is effective in determining the concentration of hydrogen gas in an aqueous solution. The method performs as effectively as the more complex and expensive electrochemical method.
Appendix
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Literature
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Ohsawa I, Ishikawa M, Takahashi K, Watanabe M, Nishimaki K, Yamagata K, Katsura K, Katayama Y, Asoh S, Ohta S: Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med. 2007, 13 (6): 688-94. 10.1038/nm1577.CrossRefPubMed
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Nakashima-Kamimura N, Mori T, Ohsawa I, Asoh S, Ohta S: Molecular hydrogen alleviates nephrotoxicity induced by an anti-cancer drug cisplatin without compromising anti-tumor activity in mice. Cancer Chemother Pharmacol. 2009, 64 (4): 753-61. 10.1007/s00280-008-0924-2.CrossRefPubMed
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Buchholz BM, Kaczorowski DJ, Sugimoto R, Yang R, Wang Y, Billiar TR, McCurry KR, Bauer AJ, Nakao A: Hydrogen inhalation ameliorates oxidative stress in transplantation induced intestinal graft injury. Am J Transplant. 2008, 8 (10): 2015-24. 10.1111/j.1600-6143.2008.02359.x.CrossRefPubMed
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Fu Y, Ito M, Fujita Y, Ito M, Ichihara M, Masuda A, Suzuki Y, Maesawa S, Kajita Y, Hirayama M, Ohsawa I, Ohta S, Ohno K: Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson's disease. Neurosci Lett. 2009, 453 (2): 81-5. 10.1016/j.neulet.2009.02.016.CrossRefPubMed
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Cai J, Kang Z, Liu K, Liu W, Li R, Zhang JH, Luo X, Sun X: Neuroprotective effects of hydrogen saline in neonatal hypoxia-ischemia rat model. Brain Res. 2009, 1256: 129-37.CrossRefPubMed
Metadata
Title
A convenient method for determining the concentration of hydrogen in water: use of methylene blue with colloidal platinum
Authors
Tomoki Seo
Ryosuke Kurokawa
Bunpei Sato
Publication date
01-12-2012
Publisher
BioMed Central
Published in
Medical Gas Research / Issue 1/2012
Electronic ISSN: 2045-9912
DOI
https://doi.org/10.1186/2045-9912-2-1

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