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Journal of Ophthalmic Inflammation and Infection

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Open Access 01-12-2013 | Original research

Novel multi-targeted polymerase chain reaction for diagnosis of presumed tubercular uveitis

Authors: Kusum Sharma, Vishali Gupta, Reema Bansal, Aman Sharma, Meera Sharma, Amod Gupta

Published in: Journal of Ophthalmic Inflammation and Infection | Issue 1/2013

Abstract

Background

The objective of this study was to report the use of multi-targeted polymerase chain reaction (PCR) in the diagnosis of presumed tubercular uveitis. Multi-targeted PCR using three targets specific for Mycobacterium tuberculosis, i.e., IS6110, MPB64, and protein b, was performed on intraocular fluid samples of 25 subjects. Nine had presumed tubercular uveitis, six had intraocular inflammation secondary to a nontubercular etiology (disease controls), and ten had no evidence of intraocular inflammation (normal controls). As described previously, response to antitubercular therapy was considered as the gold standard.

Results

Multi-targeted PCR was positive in seven out of nine patients with presumed tubercular uveitis and negative in all normal and disease controls. The sensitivity and specificity were 77.77% and 100%, respectively. For the diagnosis of presumed tubercular uveitis, multi-targeted PCR had a positive predictive value of 100% and a negative predictive value of 88.88%.

Conclusion

Multi-targeted PCR can be a valuable tool for diagnosing presumed tubercular uveitis.

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Title: Novel multi-targeted polymerase chain reaction for diagnosis of presumed tubercular uveitis
Authors: Kusum Sharma
Vishali Gupta
Reema Bansal
Aman Sharma
Meera Sharma
Amod Gupta
Publication date: 01-12-2013
Publisher: Springer Berlin Heidelberg
Published in: Journal of Ophthalmic Inflammation and Infection / Issue 1/2013
Electronic ISSN: 1869-5760
DOI: https://doi.org/10.1186/1869-5760-3-25

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Novel multi-targeted polymerase chain reaction for diagnosis of presumed tubercular uveitis

Abstract

Background

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Results

Conclusion

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