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Journal of Experimental & Clinical Cancer Research
Published in: Journal of Experimental & Clinical Cancer Research 1/2012

Open Access 01-12-2012 | Research

Increased expression of CD133 and reduced dystroglycan expression are strong predictors of poor outcome in colon cancer patients

Authors: Claudio Coco, Gian Franco Zannoni, Emanuele Caredda, Stefano Sioletic, Alma Boninsegna, Mario Migaldi, Gianluca Rizzo, Luca Reggiani Bonetti, Giannicola Genovese, Egidio Stigliano, Achille Cittadini, Alessandro Sgambato

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2012

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Abstract

Background

Expression levels of CD133, a cancer stem cell marker, and of the α-subunit of the dystroglycan (α-DG) complex, have been previously reported to be altered in colorectal cancers.

Methods

Expression levels of CD133 and α-DG were assessed by immunohistochemistry in a series of colon cancers and their prognostic significance was evaluated.

Results

Scattered cells positive for CD133 were rarely detected at the bases of the crypts in normal colonic mucosa while in cancer cells the median percentage of positive cells was 5% (range 0–80). A significant correlation was observed with pT parameter and tumor stage but not with tumor grade and N status. Recurrence and death from disease were significantly more frequent in CD133-high expressing tumors and Kaplan-Meier curves showed a significant separation between high vs low expressor groups for both disease-free (p = 0.002) and overall (p = 0.008) survival.
Expression of α-DG was reduced in a significant fraction of tumors but low α-DG staining did not correlate with any of the classical clinical-pathological parameters. Recurrence and death from the disease were significantly more frequent in α-DG-low expressing tumors and Kaplan-Meier curves showed a significant separation between high vs low expressor tumors for both disease-free (p = 0.02) and overall (p = 0.02) survival. Increased expression of CD133, but not loss of α-DG, confirmed to be an independent prognostic parameters at a multivariate analysis associated with an increased risk of recurrence (RR = 2.4; p = 0.002) and death (RR = 2.3; p = 0.003).

Conclusions

Loss of α-DG and increased CD133 expression are frequent events in human colon cancer and evaluation of CD133 expression could help to identify high-risk colon cancer patients.
Appendix
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Metadata
Title
Increased expression of CD133 and reduced dystroglycan expression are strong predictors of poor outcome in colon cancer patients
Authors
Claudio Coco
Gian Franco Zannoni
Emanuele Caredda
Stefano Sioletic
Alma Boninsegna
Mario Migaldi
Gianluca Rizzo
Luca Reggiani Bonetti
Giannicola Genovese
Egidio Stigliano
Achille Cittadini
Alessandro Sgambato
Publication date
01-12-2012
Publisher
BioMed Central
Published in
Journal of Experimental & Clinical Cancer Research / Issue 1/2012
Electronic ISSN: 1756-9966
DOI
https://doi.org/10.1186/1756-9966-31-71

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