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Open Access 01-12-2011 | Research

Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2) cell line

Authors: Sasipawan Machana, Natthida Weerapreeyakul, Sahapat Barusrux, Apiyada Nonpunya, Bungorn Sripanidkulchai, Thaweesak Thitimetharoch

Published in: Chinese Medicine | Issue 1/2011

Abstract

Background

Six plants from Thailand were evaluated for their cytotoxicity and apoptosis induction in human hepatocarcinoma (HepG2) as compared to normal African green monkey kidney epithelial cell lines.

Methods

Ethanol-water crude extracts of the six plants were tested with neutral red assay for their cytotoxicity after 24 hours of exposure to the cells. Apoptotic induction was tested in the HepG2 cells with diamidino-2-phenylindole staining. DNA fragmentation, indicative of apoptosis, was analyzed with agarose gel electrophoresis. Alkylation, indicative of DNA damage, was also evaluated in vitro by 4-(4'-nitrobenzyl) pyridine assay.

Results

The extract of Pinus kesiya showed the highest selectivity (selectivity index = 9.6) and potent cytotoxicity in the HepG2 cell line, with an IC₅₀ value of 52.0 ± 5.8 μg/ml (mean ± standard deviation). Extract of Catimbium speciosum exerted cytotoxicity with an IC₅₀ value of 55.7 ± 8.1 μg/ml. Crude extracts from Glochidion daltonii, Cladogynos orientalis, Acorus tatarinowii and Amomum villosum exhibited cytotoxicity with IC₅₀ values ranging 100-500 μg/ml. All crude extracts showed different alkylating abilities in vitro. Extracts of P. kesiya, C. speciosum and C. orientalis caused nuclei morphological changes and DNA laddering.

Conclusion

The extracts of C. speciosum, C. orientalis and P. kesiya induced apoptosis. Among the three plants, P. kesiya possessed the most robust anticancer activity, with specific selectivity against HepG2 cells.

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Title: Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2) cell line
Authors: Sasipawan Machana
Natthida Weerapreeyakul
Sahapat Barusrux
Apiyada Nonpunya
Bungorn Sripanidkulchai
Thaweesak Thitimetharoch
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Chinese Medicine / Issue 1/2011
Electronic ISSN: 1749-8546
DOI: https://doi.org/10.1186/1749-8546-6-39

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2) cell line

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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