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Radiation Oncology

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Open Access 01-12-2013 | Research

Overexpression of miRNA-21 promotes radiation-resistance of non-small cell lung cancer

Authors: Wang Xiao-chun, Wang Wei, Zhang Zhu-Bo, Zhao Jing, Tan Xiao-Gang, Luo Jian-Chao

Published in: Radiation Oncology | Issue 1/2013

Abstract

Background

MiRNA-21 was previously reported to be up-regulated in many kinds of cancer. In the present study, we want to investigate the potential role of miRNA-21 in non-small cell lung cancer.

Materials and methods

Expression of miRNA-21 was detected in 60 non-small cell lung cancer (NSCLC) samples and adjacent histologically normal tissue using RT-qPCR, Correlation between miRNA-21 expression and clinicopathological features of NSCLC was analyzed using statistical software. The effect of miRNA-21 expression on the growth and apoptosis of A549 cells induced by irradiation was examined.

Results

miRNA-21 expression increased in non-small cell lung cancer. Expression of miRNA-21 was positively associated with lymph node metastasis, clinical stage and poor prognosis. Multivariate Cox regression analysis showed that miRNA-21 was an independent prognostic factor for patients. Down-regulation of miRNA-21 inhibited proliferation and cell cycle progress of A549 cells and sensitized cells to radiation. Decreased miRNA-21 expression promoted the apoptosis of A549 cells induced by irradiation.

Conclusions

miRNA-21 may be considered as a potential novel target for future development of specific therapeutic interventions in NSCLC.

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Title: Overexpression of miRNA-21 promotes radiation-resistance of non-small cell lung cancer
Authors: Wang Xiao-chun
Wang Wei
Zhang Zhu-Bo
Zhao Jing
Tan Xiao-Gang
Luo Jian-Chao
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: Radiation Oncology / Issue 1/2013
Electronic ISSN: 1748-717X
DOI: https://doi.org/10.1186/1748-717X-8-146

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Overexpression of miRNA-21 promotes radiation-resistance of non-small cell lung cancer

Abstract

Background

Materials and methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Materials and methods

Results

Conclusions

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