Top

Published in:

Open Access 01-12-2011 | Research

MicroRNA expression profiles in human cancer cells after ionizing radiation

Authors: Olivier M Niemoeller, Maximilian Niyazi, Stefanie Corradini, Franz Zehentmayr, Minglun Li, Kirsten Lauber, Claus Belka

Published in: Radiation Oncology | Issue 1/2011

Abstract

Introduction

MicroRNAs are regulators of central cellular processes and are implicated in the pathogenesis and prognosis of human cancers. MicroRNAs also modulate responses to anti-cancer therapy. In the context of radiation oncology microRNAs were found to modulate cell death and proliferation after irradiation. However, changes in microRNA expression profiles in response to irradiation have not been comprehensively analyzed so far. The present study's intend is to present a broad screen of changes in microRNA expression following irradiation of different malignant cell lines.

Materials and methods

1100 microRNAs (Sanger miRBase release version 14.0) were analyzed in six malignant cell lines following irradiation with clinically relevant doses of 2.0 Gy. MicroRNA levels 6 hours after irradiation were compared to microRNA levels in non-irradiated cells using the "Geniom Biochip MPEA homo sapiens".

Results

Hierarchical clustering analysis revealed a pattern, which significantly (p = 0.014) discerned irradiated from non-irradiated cells. The expression levels of a number of microRNAs known to be involved in the regulation of cellular processes like apoptosis, proliferation, invasion, local immune response and radioresistance (e. g. miR-1285, miR-24-1, miR-151-5p, let-7i) displayed 2 - 3-fold changes after irradiation. Moreover, several microRNAs previously not known to be radiation-responsive were discovered.

Conclusion

Ionizing radiation induced significant changes in microRNA expression profiles in 3 glioma and 3 squamous cell carcinoma cell lines. The functional relevance of these changes is not addressed but should by analyzed by future work especially focusing on clinically relevant endpoints like radiation induced cell death, proliferation, migration and metastasis.

Available only for authorised users

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell 2004, 116: 281-297. 10.1016/S0092-8674(04)00045-5CrossRefPubMed

Lim LP, Lau NC, Garrett-Engele P, Grimson A, Schelter JM, Castle J, Bartel DP, Linsley PS, Johnson JM: Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature 2005, 433: 769-773. 10.1038/nature03315CrossRefPubMed

He L, He XY, Lim LP, De Stanchina E, Xuan ZY, Liang Y, Xue W, Zender L, Magnus J, Ridzon D, et al.: A microRNA component of the p53 tumour suppressor network. Nature 2007, 447: 1130-U1116. 10.1038/nature05939PubMedCentralCrossRefPubMed

Cimmino A, Calin GA, Fabbri M, Iorio MV, Ferracin M, Shimizu M, Wojcik SE, Aqeilan RI, Zupo S, Dono M, et al.: miR-15 and miR-16 induce apoptosis by targeting BCL2. Proceedings of the National Academy of Sciences of the United States of America 2005, 102: 13944-13949. 10.1073/pnas.0506654102PubMedCentralCrossRefPubMed

Volinia S, Calin GA, Liu CG, Ambs S, Cimmino A, Petrocca F, Visone R, Iorio M, Roldo C, Ferracin M, et al.: A microRNA expression signature of human solid tumors defines cancer gene targets. Proceedings of the National Academy of Sciences of the United States of America 2006, 103: 2257-2261. 10.1073/pnas.0510565103PubMedCentralCrossRefPubMed

Esquela-Kerscher A, Slack FJ: Oncomirs - microRNAs with a role in cancer. Nature Reviews Cancer 2006, 6: 259-269. 10.1038/nrc1840CrossRefPubMed

Calin GA, Liu CG, Sevignani C, Ferracin M, Felli N, Dumitru CD, Shimizu M, Cimmino A, Zupo S, Dono M, et al.: MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias. Proceedings of the National Academy of Sciences of the United States of America 2004, 101: 11755-11760. 10.1073/pnas.0404432101PubMedCentralCrossRefPubMed

Yanaihara N, Caplen N, Bowman E, Seike M, Kumamoto K, Yi M, Stephens RM, Okamoto A, Yokota J, Tanaka T, et al.: Unique microRNA molecular profiles in lung cancer diagnosis and prognosis. Cancer Cell 2006, 9: 189-198. 10.1016/j.ccr.2006.01.025CrossRefPubMed

Takamizawa J, Konishi H, Yanagisawa K, Tomida S, Osada H, Endoh H, Harano T, Yatabe Y, Nagino M, Nimura Y, et al.: Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival. Cancer Research 2004, 64: 3753-3756. 10.1158/0008-5472.CAN-04-0637CrossRefPubMed

10.

Weidhaas JB, Babar L, Nallur SM, Trang P, Roush S, Boehm N, Gillespie E, Slack FJ: MicroRNAs as potential agents to alter resistance to cytotoxic anticancer therapy. Cancer Research 2007, 67: 11111-11116. 10.1158/0008-5472.CAN-07-2858CrossRefPubMed

11.

Kato M, Paranjape T, Ullrich R, Nallur S, Gillespie E, Keane K, Esquela-Kerscher A, Weidhaas JB, Slack FJ: The mir-34 microRNA is required for the DNA damage response in vivo in C. elegans and in vitro in human breast cancer cells. Oncogene 2009, 28: 2419-2424. 10.1038/onc.2009.106PubMedCentralCrossRefPubMed

12.

Maes OC, An J, Sarojini H, Wu HL, Wang E: Changes in MicroRNA Expression Patterns in Human Fibroblasts After Low-LET Radiation. Journal of Cellular Biochemistry 2008, 105: 824-834. 10.1002/jcb.21878CrossRefPubMed

13.

Ilnytskyy Y, Koturbash I, Kovalchuk O: Radiation-Induced Bystander Effects In Vivo are Epigenetically Regulated in a Tissue-Specific Manner. Environmental and Molecular Mutagenesis 2009, 50: 105-113. 10.1002/em.20440CrossRefPubMed

14.

Tian S, Huang S, Wu S, Guo W, Li J, He X: MicroRNA-1285 inhibits the expression of p53 by directly targeting its 3' untranslated region. Biochem Biophys Res Commun 396: 435-439. 10.1016/j.bbrc.2010.04.112

15.

Ding J, Huang SL, Wu SQ, Zhao YJ, Liang LH, Yan MX, Ge C, Yao J, Chen TY, Wan DF, et al.: Gain of miR-151 on chromosome 8q24.3 facilitates tumour cell migration and spreading through downregulating RhoGDIA. Nature Cell Biology 2010, 12: 390-U208. 10.1038/ncb2039CrossRefPubMed

16.

Rogler CE, Levoci L, Ader T, Massimi A, Tchaikovskaya T, Norel R, Rogler LE: MicroRNA-23b cluster microRNAs regulate transforming growth factor-beta/bone morphogenetic protein signaling and liver stem cell differentiation by targeting Smads. Hepatology 2009, 50: 575-584. 10.1002/hep.22982CrossRefPubMed

17.

Blobe GC, Schiemann WP, Lodish HF: Mechanisms of disease: Role of transforming growth factor beta in human disease. New England Journal of Medicine 2000, 342: 1350-1358. 10.1056/NEJM200005043421807CrossRefPubMed

18.

Blower PE, Chung JH, Verducci JS, Lin SL, Park JK, Dai ZY, Liu CG, Schmittgen TD, Reinhold WC, Croce CM, et al.: MicroRNAs modulate the chemosensitivity of tumor cells. Molecular Cancer Therapeutics 2008, 7: 1-9. 10.1158/1535-7163.MCT-07-0573CrossRefPubMed

19.

Schmidtullrich RK, Valerie KC, Chan W, McWilliams D: Altered Expression of Epidermal Growth-Factor Receptor and Estrogen-Receptor In Mcf-7 Cells After Single and Repeated Radiation Exposures. International Journal of Radiation Oncology Biology Physics 1994, 29: 813-819. 10.1016/0360-3016(94)90570-3CrossRef

20.

Goetze K, Scholz M, Taucher-Scholz G, Mueller-Klieser W: The impact of conventional and heavy ion irradiation on tumor cell migration in vitro. International Journal of Radiation Biology 2007, 83: 889-896. 10.1080/09553000701753826CrossRefPubMed

21.

Wild-Bode C, Weller M, Rimner A, Dichgans J, Wick W: Sublethal irradiation promotes migration and invasiveness of glioma cells: Implications for radiotherapy of human glioblastoma. Cancer Research 2001, 61: 2744-2750.PubMed

22.

Johnson SM, Grosshans H, Shingara J, Byrom M, Jarvis R, Cheng A, Labourier E, Reinert KL, Brown D, Slack FJ: RAS is regulated by the let-7 MicroRNA family. Cell 2005, 120: 635-647. 10.1016/j.cell.2005.01.014CrossRefPubMed

23.

Oh JS, Kim JJ, Byun JY, Kim IA: Lin28-Let7 Modulates Radiosensitivity of Human Cancer Cells With Activation of K-Ras. International Journal of Radiation Oncology Biology Physics 2010, 76: 5-8. 10.1016/j.ijrobp.2009.08.028CrossRef

Title: MicroRNA expression profiles in human cancer cells after ionizing radiation
Authors: Olivier M Niemoeller
Maximilian Niyazi
Stefanie Corradini
Franz Zehentmayr
Minglun Li
Kirsten Lauber
Claus Belka
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Radiation Oncology / Issue 1/2011
Electronic ISSN: 1748-717X
DOI: https://doi.org/10.1186/1748-717X-6-29

Keynote webinar | Spotlight on medication adherence

Springer Medicine

MicroRNA expression profiles in human cancer cells after ionizing radiation

Abstract

Introduction

Materials and methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction

Materials and methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 1/2011

Dysphagia in head and neck cancer patients following intensity modulated radiotherapy (IMRT)

Radioresistance of human glioma spheroids and expression of HSP70, p53 and EGFr

Spinal cord stimulators and radiotherapy: First case report and practice guidelines

Successful radiation treatment of anaplastic thyroid carcinoma metastatic to the right cardiac atrium and ventricle in a pacemaker-dependent patient

Concurrent image-guided intensity modulated radiotherapy and chemotherapy following neoadjuvant chemotherapy for locally advanced nasopharyngeal carcinoma

Dosimetric evaluation of Acuros XB Advanced Dose Calculation algorithm in heterogeneous media