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Published in: Radiation Oncology 1/2007

Open Access 01-12-2007 | Methodology

Counting colonies of clonogenic assays by using densitometric software

Authors: Maximilian Niyazi, Ismat Niyazi, Claus Belka

Published in: Radiation Oncology | Issue 1/2007

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Abstract

Clonogenic assays are a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumour cells. A colony is defined as a cluster of at least 50 cells which can often only be determined microscopically. The process of counting colonies is very extensive work and so we developed software that is able to count the colonies automatically from scanned flasks. This software is made freely available by us with a detailed description how to use and install the necessary features.
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Metadata
Title
Counting colonies of clonogenic assays by using densitometric software
Authors
Maximilian Niyazi
Ismat Niyazi
Claus Belka
Publication date
01-12-2007
Publisher
BioMed Central
Published in
Radiation Oncology / Issue 1/2007
Electronic ISSN: 1748-717X
DOI
https://doi.org/10.1186/1748-717X-2-4

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