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Molecular Pain

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Open Access 01-12-2012 | Research

Satellite glial cell P2Y₁₂ receptor in the trigeminal ganglion is involved in lingual neuropathic pain mechanisms in rats

Authors: Ayano Katagiri, Masamichi Shinoda, Kuniya Honda, Akira Toyofuku, Barry J Sessle, Koichi Iwata

Published in: Molecular Pain | Issue 1/2012

Abstract

Background

It has been reported that the P2Y₁₂ receptor (P2Y₁₂R) is involved in satellite glial cells (SGCs) activation, indicating that P2Y₁₂R expressed in SGCs may play functional roles in orofacial neuropathic pain mechanisms. However, the involvement of P2Y₁₂R in orofacial neuropathic pain mechanisms is still unknown. We therefore studied the reflex to noxious mechanical or heat stimulation of the tongue, P2Y₁₂R and glial fibrillary acidic protein (GFAP) immunohistochemistries in the trigeminal ganglion (TG) in a rat model of unilateral lingual nerve crush (LNC) to evaluate role of P2Y₁₂R in SGC in lingual neuropathic pain.

Results

The head-withdrawal reflex thresholds to mechanical and heat stimulation of the lateral tongue were significantly decreased in LNC-rats compared to sham-rats. These nocifensive effects were apparent on day 1 after LNC and lasted for 17 days. On days 3, 9, 15 and 21 after LNC, the mean relative number of TG neurons encircled with GFAP-immunoreactive (IR) cells significantly increased in the ophthalmic, maxillary and mandibular branch regions of TG. On day 3 after LNC, P2Y₁₂R expression occurred in GFAP-IR cells but not neuronal nuclei (NeuN)-IR cells (i.e. neurons) in TG. After 3 days of successive administration of the P2Y₁₂R antagonist MRS2395 into TG in LNC-rats, the mean relative number of TG neurons encircled with GFAP-IR cells was significantly decreased coincident with a significant reversal of the lowered head-withdrawal reflex thresholds to mechanical and heat stimulation of the tongue compared to vehicle-injected rats. Furthermore, after 3 days of successive administration of the P2YR agonist 2-MeSADP into the TG in naïve rats, the mean relative number of TG neurons encircled with GFAP-IR cells was significantly increased and head-withdrawal reflex thresholds to mechanical and heat stimulation of the tongue were significantly decreased in a dose-dependent manner compared to vehicle-injected rats.

Conclusions

The present findings provide the first evidence that the activation of P2Y₁₂R in SGCs of TG following lingual nerve injury is involved in the enhancement of TG neuron activity and nocifensive reflex behavior, resulting in neuropathic pain in the tongue.

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Title: Satellite glial cell P2Y12 receptor in the trigeminal ganglion is involved in lingual neuropathic pain mechanisms in rats
Authors: Ayano Katagiri
Masamichi Shinoda
Kuniya Honda
Akira Toyofuku
Barry J Sessle
Koichi Iwata
Publication date: 01-12-2012
Publisher: BioMed Central
Published in: Molecular Pain / Issue 1/2012
Electronic ISSN: 1744-8069
DOI: https://doi.org/10.1186/1744-8069-8-23

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Satellite glial cell P2Y₁₂ receptor in the trigeminal ganglion is involved in lingual neuropathic pain mechanisms in rats

Abstract

Background

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Results

Conclusions

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