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Open Access 01-12-2011 | Short report

Phenotypes of pain behavior in phospholipase C-related but catalytically inactive protein type 1 knockout mice

Authors: Keisuke Migita, Masahiko Tomiyama, Junko Yamada, Masashi Fukuzawa, Takashi Kanematsu, Masato Hirata, Shinya Ueno

Published in: Molecular Pain | Issue 1/2011

Abstract

Phospholipase C-related inactive protein (PRIP) plays important roles in trafficking to the plasma membrane of GABA_A receptor, which is involved in the dominant inhibitory neurotransmission in the spinal cord and plays an important role in nociceptive transmission. However, the role of PRIP in pain sensation remains unknown. In this study, we investigated the phenotypes of pain behaviors in PRIP type 1 knockout (PRIP-1^-/-) mice. The mutant mice showed hyperalgesic responses in the second phase of the formalin test and the von Frey test as compared with those in wild-type mice. In situ hybridization studies of GABA_A receptors revealed significantly decreased expression of γ2 subunit mRNA in the dorsal and ventral horns of the spinal cord in PRIP-1^-/-mice, but no difference in α1 subunit mRNA expression. β2 subunit mRNA expression was significantly higher in PRIP-1^-/-mice than in wild-type mice in all areas of the spinal cord. On the other hand, the slow decay time constant for the spontaneous inhibitory current was significantly increased by treatment with diazepam in wild-type mice, but not in PRIP-1^-/-mice. These results suggest that PRIP-1^-/-mice exhibit the changes of the function and subunits expression of GABA_A receptor in the spinal cord, which may be responsible for abnormal pain sensation in these mice.

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Title: Phenotypes of pain behavior in phospholipase C-related but catalytically inactive protein type 1 knockout mice
Authors: Keisuke Migita
Masahiko Tomiyama
Junko Yamada
Masashi Fukuzawa
Takashi Kanematsu
Masato Hirata
Shinya Ueno
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Molecular Pain / Issue 1/2011
Electronic ISSN: 1744-8069
DOI: https://doi.org/10.1186/1744-8069-7-79

2024 ASCO Annual Meeting

Springer Medicine

Phenotypes of pain behavior in phospholipase C-related but catalytically inactive protein type 1 knockout mice

Abstract

2024 ASCO Annual Meeting

Springer Medicine

Abstract

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