Top

Virology Journal

Published in:

Open Access 01-12-2010 | Research

Use of monoclonal antibodies against Hendra and Nipah viruses in an antigen capture ELISA

Authors: Cheng-Feng Chiang, Michael K Lo, Paul A Rota, Christina F Spiropoulou, Pierre E Rollin

Published in: Virology Journal | Issue 1/2010

Abstract

Background

Outbreaks of Hendra (HeV) and Nipah (NiV) viruses have been reported starting in 1994 and 1998, respectively. Both viruses are capable of causing fatal disease in humans and effecting great economical loss in the livestock industry.

Results

Through screening of hybridomas derived from mice immunized with γ-irradiated Nipah virus, we identified two secreted antibodies; one reactive with the nucleocapsid (N) protein and the other, the phosphoprotein (P) of henipaviruses. Epitope mapping and protein sequence alignments between NiV and HeV suggest the last 14 amino acids of the carboxyl terminus of the N protein is the target of the anti-N antibody. The anti-P antibody recognizes an epitope in the amino-terminal half of P protein. These monoclonal antibodies were used to develop two antigen capture ELISAs, one for virus detection and the other for differentiation between NiV and HeV. The lower limit of detection of the capture assay with both monoclonal antibodies was 400 pfu. The anti-N antibody was used to successfully detect NiV in a lung tissue suspension from an infected pig.

Conclusion

The antigen capture ELISA developed is potentially affordable tool to provide rapid detection and differentiation between the henipaviruses.

Available only for authorised users

Wild TF: Henipaviruses: a new family of emerging Paramyxoviruses. Pathol Biol. 2009, 57: 188-196. 10.1016/j.patbio.2008.04.006.PubMedCrossRef

Lo MK, Rota PA: The emergence of Nipah virus, a highly pathogenic paramyxovirus. J Clin Virol. 2008, 43: 396-400. 10.1016/j.jcv.2008.08.007.PubMedCrossRef

Guillaume V, Wong KT, Looi RY, Georges-Courbot MC, Barrot L, Buckland R, Wild TF, Horvat B: Acute Hendra virus infection: Analysis of the pathogenesis and passive antibody protection in the hamster model. Virology. 2009, 387: 459-465. 10.1016/j.virol.2009.03.001.PubMedCrossRef

Hsu VP, Hossain MJ, Parashar UD, Ali MM, Ksiazek TG, Kuzmin I, Niezgoda M, Rupprecht C, Bresee J, Breiman RF: Nipah virus encephalitis reemergence, Bangladesh. Emerg Infect Dis. 2004, 10: 2082-2087.PubMedPubMedCentralCrossRef

Playford EG, McCall B, Smith G, Slinko V, Allen G, Smith I, Moore F, Taylor C, Kung YH, Field H: Human Hendra virus encephalitis associated with equine outbreak, Australia, 2008. Emerg Infect Dis. 2010, 16: 219-223.PubMedPubMedCentralCrossRef

Wacharapluesadee S, Boongird K, Wanghongsa S, Ratanasetyuth N, Supavonwong P, Saengsen D, Gongal GN, Hemachudha T: A longitudinal study of the prevalence of Nipah virus in Pteropus lylei bats in Thailand: Evidence for seasonal preference in disease transmission. Vector Borne Zoonotic Dis. 2010, 10: 183-190. 10.1089/vbz.2008.0105.PubMedCrossRef

Yob JM, Field H, Rashdi AM, Morrissy C, van der Heide B, Rota P, bin Adzhar A, White J, Daniels P, Jamaluddin A, Ksiazek T: Nipah virus infection in bats (order Chiroptera) in peninsular Malaysia. Emerg Infect Dis. 2001, 7: 439-441.PubMedPubMedCentralCrossRef

Field H, Young P, Yob JM, Mills J, Hall L, Mackenzie J: The natural history of Hendra and Nipah viruses. Microbes Infect. 2001, 3: 307-314. 10.1016/S1286-4579(01)01384-3.PubMedCrossRef

Eaton BT, Broder CC, Middleton D, Wang LF: Hendra and Nipah viruses: different and dangerous. Nat Rev Microbiol. 2006, 4: 23-35. 10.1038/nrmicro1323.PubMedCrossRef

10.

Chua KB, Bellini WJ, Rota PA, Harcourt BH, Tamin A, Lam SK, Ksiazek TG, Rollin PE, Zaki SR, Shieh W, et al: Nipah virus: a recently emergent deadly paramyxovirus. Science. 2000, 288: 1432-1435. 10.1126/science.288.5470.1432.PubMedCrossRef

11.

Murray K, Selleck P, Hooper P, Hyatt A, Gould A, Gleeson L, Westbury H, Hiley L, Selvey L, Rodwell B: A morbillivirus that caused fatal disease in horses and humans. Science. 1995, 268: 94-97. 10.1126/science.7701348.PubMedCrossRef

12.

Harcourt BH, Lowe L, Tamin A, Liu X, Bankamp B, Bowden N, Rollin PE, Comer JA, Ksiazek TG, Hossain MJ, et al: Genetic characterization of Nipah virus, Bangladesh, 2004. Emerg Infect Dis. 2005, 11: 1594-1597.PubMedPubMedCentralCrossRef

13.

Hossain MJ, Gurley ES, Montgomery JM, Bell M, Carroll DS, Hsu VP, Formenty P, Croisier A, Bertherat E, Faiz MA, et al: Clinical presentation of Nipah virus infection in Bangladesh. Clin Infect Dis. 2008, 46: 977-984. 10.1086/529147.PubMedCrossRef

14.

Chadha MS, Comer JA, Lowe L, Rota PA, Rollin PE, Bellini WJ, Ksiazek TG, Mishra A: Nipah virus-associated encephalitis outbreak, Siliguri, India. Emerg Infect Dis. 2006, 12: 235-240.PubMedPubMedCentralCrossRef

15.

Voelker R: Promising antibody fights lethal viruses. JAMA. 2009, 302: 2643-10.1001/jama.2009.1843.CrossRef

16.

Daniels P, Ksiazek T, Eaton BT: Laboratory diagnosis of Nipah and Hendra virus infections. Microbes Infect. 2001, 3: 289-295. 10.1016/S1286-4579(01)01382-X.PubMedCrossRef

17.

Bossart KN, Zhu Z, Middleton D, Klippel J, Crameri G, Bingham J, McEachern JA, Green D, Hancock TJ, Chan YP, et al: A neutralizing human monoclonal antibody protects against lethal disease in a new ferret model of acute Nipah virus infection. PLoS Pathog. 2009, 5: e1000642-10.1371/journal.ppat.1000642.PubMedPubMedCentralCrossRef

18.

Harcourt BH, Tamin A, Ksiazek TG, Rollin PE, Anderson LJ, Bellini WJ, Rota PA: Molecular characterization of Nipah virus, a newly emergent paramyxovirus. Virology. 2000, 271: 334-349. 10.1006/viro.2000.0340.PubMedCrossRef

19.

Wang L, Harcourt BH, Yu M, Tamin A, Rota PA, Bellini WJ, Eaton BT: Molecular biology of Hendra and Nipah viruses. Microbes Infect. 2001, 3: 279-287. 10.1016/S1286-4579(01)01381-8.PubMedCrossRef

20.

Mayo MA: Names of viruses and virus species - an editorial note. Arch Virol. 2002, 147: 1463-1464. 10.1007/s007050200042.PubMedCrossRef

21.

Shiell BJ, Gardner DR, Crameri G, Eaton BT, Michalski WP: Sites of phosphorylation of P and V proteins from Hendra and Nipah viruses: newly emerged members of Paramyxoviridae. Virus Res. 2003, 92: 55-65. 10.1016/S0168-1702(02)00313-1.PubMedCrossRef

22.

Chen JM, Yu M, Morrissy C, Zhao YG, Meehan G, Sun YX, Wang QH, Zhang W, Wang LF, Wang ZL: A comparative indirect ELISA for the detection of henipavirus antibodies based on a recombinant nucleocapsid protein expressed in Escherichia coli. J Virol Methods. 2006, 136: 273-276. 10.1016/j.jviromet.2006.05.003.PubMedCrossRef

23.

Eshaghi M, Tan WS, Ong ST, Yusoff K: Purification and characterization of Nipah virus nucleocapsid protein produced in insect cells. J Clin Microbiol. 2005, 43: 3172-3177. 10.1128/JCM.43.7.3172-3177.2005.PubMedPubMedCentralCrossRef

24.

Juozapaitis M, Serva A, Zvirbliene A, Slibinskas R, Staniulis J, Sasnauskas K, Shiell BJ, Wang LF, Michalski WP: Generation of henipavirus nucleocapsid proteins in yeast Saccharomyces cerevisiae. Virus Res. 2007, 124: 95-102. 10.1016/j.virusres.2006.10.008.PubMedCrossRef

25.

Yu F, Khairullah NS, Inoue S, Balasubramaniam V, Berendam SJ, Teh LK, Ibrahim NS, Abdul Rahman S, Hassan SS, Hasebe F, et al: Serodiagnosis using recombinant Nipah virus nucleocapsid protein expressed in Escherichia coli. J Clin Microbiol. 2006, 44: 3134-3138. 10.1128/JCM.00693-06.PubMedPubMedCentralCrossRef

26.

Crameri G, Wang LF, Morrissy C, White J, Eaton BT: A rapid immune plaque assay for the detection of Hendra and Nipah viruses and anti-virus antibodies. J Virol Methods. 2002, 99: 41-51. 10.1016/S0166-0934(01)00377-9.PubMedCrossRef

27.

Tamin A, Harcourt BH, Lo MK, Roth JA, Wolf MC, Lee B, Weingartl H, Audonnet JC, Bellini WJ, Rota PA: Development of a neutralization assay for Nipah virus using pseudotype particles. J Virol Methods. 2009, 160: 1-6. 10.1016/j.jviromet.2009.02.025.PubMedPubMedCentralCrossRef

28.

Guillaume V, Lefeuvre A, Faure C, Marianneau P, Buckland R, Lam SK, Wild TF, Deubel V: Specific detection of Nipah virus using real-time RT-PCR (TaqMan). J Virol Methods. 2004, 120: 229-237. 10.1016/j.jviromet.2004.05.018.PubMedCrossRef

29.

Gurley ES, Montgomery JM, Hossain MJ, Bell M, Azad AK, Islam MR, Molla MA, Carroll DS, Ksiazek TG, Rota PA, et al: Person-to-person transmission of Nipah virus in a Bangladeshi community. Emerg Infect Dis. 2007, 13: 1031-1037.PubMedPubMedCentralCrossRef

30.

Muller JD, McEachern JA, Bossart KN, Hansson E, Yu M, Clavijo A, Hammond JM, Wang LF: Serotype-independent detection of foot-and-mouth disease virus. J Virol Methods. 2008, 151: 146-153. 10.1016/j.jviromet.2008.03.011.PubMedCrossRef

31.

Ferris NP, Abrescia NG, Stuart DI, Jackson T, Burman A, King DP, Paton DJ: Utility of recombinant integrin alpha v beta6 as a capture reagent in immunoassays for the diagnosis of foot-and-mouth disease. J Virol Methods. 2005, 127: 69-79. 10.1016/j.jviromet.2005.02.014.PubMedCrossRef

32.

Kulkarni S, Volchkova V, Basler CF, Palese P, Volchkov VE, Shaw ML: Nipah virus edits its P gene at high frequency to express the V and W proteins. J Virol. 2009, 83: 3982-3987. 10.1128/JVI.02599-08.PubMedPubMedCentralCrossRef

33.

Sleeman K, Bankamp B, Hummel KB, Lo MK, Bellini WJ, Rota PA: The C, V and W proteins of Nipah virus inhibit minigenome replication. J Gen Virol. 2008, 89: 1300-1308. 10.1099/vir.0.83582-0.PubMedCrossRef

34.

Lo MK, Harcourt BH, Mungall BA, Tamin A, Peeples ME, Bellini WJ, Rota PA: Determination of the henipavirus phosphoprotein gene mRNA editing frequencies and detection of the C, V and W proteins of Nipah virus in virus-infected cells. J Gen Virol. 2009, 90: 398-404. 10.1099/vir.0.007294-0.PubMedCrossRef

35.

Iehle C, Razafitrimo G, Razainirina J, Andriaholinirina N, Goodman SM, Faure C, Georges-Courbot MC, Rousset D, Reynes JM: Henipavirus and Tioman virus antibodies in pteropodid bats, Madagascar. Emerg Infect Dis. 2007, 13: 159-161. 10.3201/eid1301.060791.PubMedPubMedCentralCrossRef

36.

Drexler JF, Corman VM, Gloza-Rausch F, Seebens A, Annan A, Ipsen A, Kruppa T, Muller MA, Kalko EK, Adu-Sarkodie Y, et al: Henipavirus RNA in African bats. PLoS One. 2009, 4: e6367-10.1371/journal.pone.0006367.PubMedPubMedCentralCrossRef

37.

Hayman DT, Suu-Ire R, Breed AC, McEachern JA, Wang L, Wood JL, Cunningham AA: Evidence of henipavirus infection in West African fruit bats. PLoS One. 2008, 3: e2739-10.1371/journal.pone.0002739.PubMedPubMedCentralCrossRef

38.

Berhane Y, Berry JD, Ranadheera C, Marszal P, Nicolas B, Yuan X, Czub M, Weingartl H: Production and characterization of monoclonal antibodies against binary ethylenimine inactivated Nipah virus. J Virol Methods. 2006, 132: 59-68. 10.1016/j.jviromet.2005.09.005.PubMedCrossRef

39.

Zhu Z, Bossart KN, Bishop KA, Crameri G, Dimitrov AS, McEachern JA, Feng Y, Middleton D, Wang LF, Broder CC, Dimitrov DS: Exceptionally potent cross-reactive neutralization of Nipah and Hendra viruses by a human monoclonal antibody. J Infect Dis. 2008, 197: 846-853. 10.1086/528801.PubMedCrossRef

40.

Xiao C, Liu Y, Jiang Y, Magoffin DE, Guo H, Xuan H, Wang G, Wang LF, Tu C: Monoclonal antibodies against the nucleocapsid proteins of henipaviruses: production, epitope mapping and application in immunohistochemistry. Arch Virol. 2008, 153: 273-281. 10.1007/s00705-007-1079-x.PubMedCrossRef

41.

Chan YP, Koh CL, Lam SK, Wang LF: Mapping of domains responsible for nucleocapsid protein-phosphoprotein interaction of henipaviruses. J Gen Virol. 2004, 85: 1675-1684. 10.1099/vir.0.19752-0.PubMedCrossRef

42.

Eshaghi M, Tan WS, Yusoff K: Identification of epitopes in the nucleocapsid protein of Nipah virus using a linear phage-displayed random peptide library. J Med Virol. 2005, 75: 147-152. 10.1002/jmv.20249.PubMedCrossRef

43.

Ksiazek TG, Rollin PE, Jahrling PB, Johnson E, Dalgard DW, Peters CJ: Enzyme immunosorbent assay for Ebola virus antigens in tissues of infected primates. J Clin Microbiol. 1992, 30: 947-950.PubMedPubMedCentral

44.

Saijo M, Georges-Courbot MC, Fukushi S, Mizutani T, Philippe M, Georges AJ, Kurane I, Morikawa S: Marburgvirus nucleoprotein-capture enzyme-linked immunosorbent assay using monoclonal antibodies to recombinant nucleoprotein: detection of authentic Marburgvirus. Jpn J Infect Dis. 2006, 59: 323-325.PubMed

45.

Maisner A, Neufeld J, Weingartl H: Organ- and endotheliotropism of Nipah virus infections in vivo and in vitro. Thromb Haemost. 2009, 102: 1014-1023.PubMed

46.

Bossart KN, McEachern JA, Hickey AC, Choudhry V, Dimitrov DS, Eaton BT, Wang LF: Neutralization assays for differential henipavirus serology using Bio-Plex protein array systems. J Virol Methods. 2007, 142: 29-40. 10.1016/j.jviromet.2007.01.003.PubMedCrossRef

47.

Tong S, Chern SW, Li Y, Pallansch MA, Anderson LJ: Sensitive and broadly reactive reverse transcription-PCR assays to detect novel paramyxoviruses. J Clin Microbiol. 2008, 46: 2652-2658. 10.1128/JCM.00192-08.PubMedPubMedCentralCrossRef

48.

Gupta M, Mahanty S, Bray M, Ahmed R, Rollin PE: Passive transfer of antibodies protects immunocompetent and imunodeficient mice against lethal Ebola virus infection without complete inhibition of viral replication. J Virol. 2001, 75: 4649-4654. 10.1128/JVI.75.10.4649-4654.2001.PubMedPubMedCentralCrossRef

49.

Porzig R, Singer D, Hoffmann R: Epitope mapping of mAbs AT8 and Tau5 directed against hyperphosphorylated regions of the human tau protein. Biochem Biophys Res Commun. 2007, 358: 644-649. 10.1016/j.bbrc.2007.04.187.PubMedCrossRef

50.

Ksiazek TG, Rollin PE, Williams AJ, Bressler DS, Martin ML, Swanepoel R, Burt FJ, Leman PA, Khan AS, Rowe AK, et al: Clinical virology of Ebola hemorrhagic fever (EHF): virus, virus antigen, and IgG and IgM antibody findings among EHF patients in Kikwit, Democratic Republic of the Congo, 1995. J Infect Dis. 1999, 179 (Suppl 1): S177-187. 10.1086/514321.PubMedCrossRef

Title: Use of monoclonal antibodies against Hendra and Nipah viruses in an antigen capture ELISA
Authors: Cheng-Feng Chiang
Michael K Lo
Paul A Rota
Christina F Spiropoulou
Pierre E Rollin
Publication date: 01-12-2010
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2010
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/1743-422X-7-115

Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin

Prof. Florian Limbourg

Prof. Anoop Chauhan

Developed by: Springer Medicine

Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 1/2010

Co-receptor usage and prediction of v3 genotyping algorithms in hiv-1 subtype b' from paid blood donors experienced anti-retroviral therapy in chinese central province

Erratum to: The state of the art of adeno-associated virus-based vectors in gene therapy

Identification of super-infected Aedes triseriatus mosquitoes collected as eggs from the field and partial characterization of the infecting La Crosse viruses

A novel method for purifying bluetongue virus with high purity by co-immunoprecipitation with agarose protein A

Molecular characterization of the virulent infectious hematopoietic necrosis virus (IHNV) strain 220-90

Sequence analysis of the Epstein-Barr virus (EBV) BRLF1 gene in nasopharyngeal and gastric carcinomas

Keynote webinar | Spotlight on medication adherence

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

At a glance: The STEP trials