Published in:
Open Access
01-12-2013 | Short report
A novel bocavirus in canine liver
Authors:
Linlin Li, Patricia A Pesavento, Christian M Leutenegger, Marko Estrada, Lark L Coffey, Samia N Naccache, Erik Samayoa, Charles Chiu, Jianming Qiu, Chunlin Wang, Xutao Deng, Eric Delwart
Published in:
Virology Journal
|
Issue 1/2013
Login to get access
Abstract
Background
Bocaviruses are classified as a genus within the Parvoviridae family of single-stranded DNA viruses and are pathogenic in some mammalian species. Two species have been previously reported in dogs, minute virus of canines (MVC), associated with neonatal diseases and fertility disorders; and Canine bocavirus (CBoV), associated with respiratory disease.
Findings
In this study using deep sequencing of enriched viral particles from the liver of a dog with severe hemorrhagic gastroenteritis, necrotizing vasculitis, granulomatous lymphadenitis and anuric renal failure, we identified and characterized a novel bocavirus we named Canine bocavirus 3 (CnBoV3). The three major ORFs of CnBoV3 (NS1, NP1 and VP1) shared less than 60% aa identity with those of other bocaviruses qualifying it as a novel species based on ICTV criteria. Inverse PCR showed the presence of concatemerized or circular forms of the genome in liver.
Conclusions
We genetically characterized a bocavirus in a dog liver that is highly distinct from prior canine bocaviruses found in respiratory and fecal samples. Its role in this animal’s complex disease remains to be determined.