Skip to main content
Top
Published in: Journal of the International Society of Sports Nutrition 1/2008

Open Access 01-12-2008 | Research article

Effect of carbohydrate-protein supplement timing on acute exercise-induced muscle damage

Authors: James P White, Jacob M Wilson, Krista G Austin, Beau K Greer, Noah St John, Lynn B Panton

Published in: Journal of the International Society of Sports Nutrition | Issue 1/2008

Login to get access

Abstract

Purpose

To determine if timing of a supplement would have an effect on muscle damage, function and soreness.

Methods

Twenty-seven untrained men (21 ± 3 yrs) were given a supplement before or after exercise. Subjects were randomly assigned to a pre exercise (n = 9), received carbohydrate/protein drink before exercise and placebo after, a post exercise (n = 9), received placebo before exercise and carbohydrate/protein drink after, or a control group (n = 9), received placebo before and after exercise. Subjects performed 50 eccentric quadriceps contractions on an isokinetic dynamometer. Tests for creatine kinase (CK), maximal voluntary contraction (MVC) and muscle soreness were recorded before exercise and at six, 24, 48, 72, and 96 h post exercise. Repeated measures ANOVA were used to analyze data.

Results

There were no group by time interactions however, CK significantly increased for all groups when compared to pre exercise (101 ± 43 U/L) reaching a peak at 48 h (661 ± 1178 U/L). MVC was significantly reduced at 24 h by 31.4 ± 14.0%. Muscle soreness was also significantly increased from pre exercise peaking at 48 h.

Conclusion

Eccentric exercise caused significant muscle damage, loss of strength, and soreness; however timing of ingestion of carbohydrate/protein supplement had no effect.
Appendix
Available only for authorised users
Literature
1.
go back to reference Saunders MJ, Kane MD, Todd MK: Effects of a carbohydrate-protein beverage on cycling endurance and muscle damage. Med Sci Sports Exerc. 2004, 36: 1233-1238. 10.1249/01.MSS.0000132377.66177.9F.CrossRefPubMed Saunders MJ, Kane MD, Todd MK: Effects of a carbohydrate-protein beverage on cycling endurance and muscle damage. Med Sci Sports Exerc. 2004, 36: 1233-1238. 10.1249/01.MSS.0000132377.66177.9F.CrossRefPubMed
2.
go back to reference Seifert JG, Kipp RW, Amann M, Gazal O: Muscle damage, fluid ingestion, and energy supplementation during recreational alpine skiing. Int J Sport Nutr Exerc Metab. 2005, 15: 528-536.PubMed Seifert JG, Kipp RW, Amann M, Gazal O: Muscle damage, fluid ingestion, and energy supplementation during recreational alpine skiing. Int J Sport Nutr Exerc Metab. 2005, 15: 528-536.PubMed
3.
go back to reference Wojcik JR, Walber-Rankin J, Smith LL, Gwazdauskas FC: Comparison of carbohydrate and milk-based beverages on muscle damage and glycogen following exercise. Int J Sport Nutr Exerc Metab. 2001, 11: 406-419.PubMed Wojcik JR, Walber-Rankin J, Smith LL, Gwazdauskas FC: Comparison of carbohydrate and milk-based beverages on muscle damage and glycogen following exercise. Int J Sport Nutr Exerc Metab. 2001, 11: 406-419.PubMed
4.
go back to reference Belcastro AN, Shewchuk LD, Raj DA: Exercise-induced muscle injury: a calpain hypothesis. Mol Cell Biochem. 1998, 179: 135-145. 10.1023/A:1006816123601.CrossRefPubMed Belcastro AN, Shewchuk LD, Raj DA: Exercise-induced muscle injury: a calpain hypothesis. Mol Cell Biochem. 1998, 179: 135-145. 10.1023/A:1006816123601.CrossRefPubMed
5.
go back to reference Evans WJ, Meredith CN, Cannon JG, Dinarello CA, Frontera WR, Hughes VA, Jones BH, Knuttgen HG: Metabolic changes following eccentric exercise in trained and untrained men. J Appl Physiol. 1986, 61: 1864-1868.PubMed Evans WJ, Meredith CN, Cannon JG, Dinarello CA, Frontera WR, Hughes VA, Jones BH, Knuttgen HG: Metabolic changes following eccentric exercise in trained and untrained men. J Appl Physiol. 1986, 61: 1864-1868.PubMed
6.
go back to reference Friden J, Sjostrom M, Ekblom B: Myofibrillar damage following intense eccentric exercise in man. Int J Sports Med. 1983, 4: 170-176.CrossRefPubMed Friden J, Sjostrom M, Ekblom B: Myofibrillar damage following intense eccentric exercise in man. Int J Sports Med. 1983, 4: 170-176.CrossRefPubMed
7.
go back to reference Candow DG, Chilibeck PD, Facci M, Abeysekara S, Zello GA: Protein supplementation before and after resistance training in older men. Eur J Appl Physiol. 2006, 97: 548-556. 10.1007/s00421-006-0223-8.CrossRefPubMed Candow DG, Chilibeck PD, Facci M, Abeysekara S, Zello GA: Protein supplementation before and after resistance training in older men. Eur J Appl Physiol. 2006, 97: 548-556. 10.1007/s00421-006-0223-8.CrossRefPubMed
8.
go back to reference Nosaka K, Chapman D, Newton M, Sacco P: Is isometric strength loss immediately after eccentric exercise related to changes in indirect markers of muscle damage?. Appl Physiol Nutr Metab. 2006, 31: 313-319. 10.1139/H06-005.CrossRefPubMed Nosaka K, Chapman D, Newton M, Sacco P: Is isometric strength loss immediately after eccentric exercise related to changes in indirect markers of muscle damage?. Appl Physiol Nutr Metab. 2006, 31: 313-319. 10.1139/H06-005.CrossRefPubMed
9.
go back to reference Coombes JS, McNaughton LR: Effects of branched-chain amino acid supplementation on serum creatine kinase and lactate dehydrogenase after prolonged exercise. J Sports Med Phys Fitness. 2000, 40: 240-246.PubMed Coombes JS, McNaughton LR: Effects of branched-chain amino acid supplementation on serum creatine kinase and lactate dehydrogenase after prolonged exercise. J Sports Med Phys Fitness. 2000, 40: 240-246.PubMed
10.
go back to reference Nissen S, Sharp R, Ray M, Rathmacher JA, Rice D, Fuller JC, Connelly AS, Abumrad N: Effect of leucine metabolite beta-hydroxy-beta-methylbutyrate on muscle metabolism during resistance-exercise training. J Appl Physiol. 1996, 81: 2095-2104.PubMed Nissen S, Sharp R, Ray M, Rathmacher JA, Rice D, Fuller JC, Connelly AS, Abumrad N: Effect of leucine metabolite beta-hydroxy-beta-methylbutyrate on muscle metabolism during resistance-exercise training. J Appl Physiol. 1996, 81: 2095-2104.PubMed
11.
go back to reference Shimomura Y, Yamamoto Y, Bajotto G, Sato J, Murakami T, Shimomura N, Kobayashi H, Mawatari K: Nutraceutical effects of branched-chain amino acids on skeletal muscle. J Nutr. 2006, 136: 529S-532S.PubMed Shimomura Y, Yamamoto Y, Bajotto G, Sato J, Murakami T, Shimomura N, Kobayashi H, Mawatari K: Nutraceutical effects of branched-chain amino acids on skeletal muscle. J Nutr. 2006, 136: 529S-532S.PubMed
12.
go back to reference Bird SP, Tarpenning KM, Marino FE: Independent and combined effects of liquid carbohydrate/essential amino acid ingestion on hormonal and muscular adaptations following resistance training in untrained men. Eur J Appl Physiol. 2006, 97: 225-238. 10.1007/s00421-005-0127-z.CrossRefPubMed Bird SP, Tarpenning KM, Marino FE: Independent and combined effects of liquid carbohydrate/essential amino acid ingestion on hormonal and muscular adaptations following resistance training in untrained men. Eur J Appl Physiol. 2006, 97: 225-238. 10.1007/s00421-005-0127-z.CrossRefPubMed
13.
go back to reference Bird SP, Tarpenning KM, Marino FE: Liquid carbohydrate/essential amino acid ingestion during a short-term bout of resistance exercise suppresses myofibrillar protein degradation. Metabolism. 2006, 55: 570-577. 10.1016/j.metabol.2005.11.011.CrossRefPubMed Bird SP, Tarpenning KM, Marino FE: Liquid carbohydrate/essential amino acid ingestion during a short-term bout of resistance exercise suppresses myofibrillar protein degradation. Metabolism. 2006, 55: 570-577. 10.1016/j.metabol.2005.11.011.CrossRefPubMed
14.
go back to reference Behm DG, Baker KM, Kelland R, Lomond J: The effect of muscle damage on strength and fatigue deficits. J Strength Cond Res. 2001, 15: 255-263. 10.1519/1533-4287(2001)015<0255:TEOMDO>2.0.CO;2.PubMed Behm DG, Baker KM, Kelland R, Lomond J: The effect of muscle damage on strength and fatigue deficits. J Strength Cond Res. 2001, 15: 255-263. 10.1519/1533-4287(2001)015<0255:TEOMDO>2.0.CO;2.PubMed
15.
go back to reference Helman EE, Huff-Lonergan E, Davenport GM, Lonergan SM: Effect of dietary protein on calpastatin in canine skeletal muscle. J Anim Sci. 2003, 81: 2199-2205.PubMed Helman EE, Huff-Lonergan E, Davenport GM, Lonergan SM: Effect of dietary protein on calpastatin in canine skeletal muscle. J Anim Sci. 2003, 81: 2199-2205.PubMed
16.
go back to reference Millard-Stafford M, Warren GL, Thomas LM, Doyle JA, Snow T, Hitchcock K: Recovery from run training: efficacy of a carbohydrate-protein beverage?. Int J Sport Nutr Exerc Metab. 2005, 15: 610-624.PubMed Millard-Stafford M, Warren GL, Thomas LM, Doyle JA, Snow T, Hitchcock K: Recovery from run training: efficacy of a carbohydrate-protein beverage?. Int J Sport Nutr Exerc Metab. 2005, 15: 610-624.PubMed
17.
go back to reference Jackson AS, Pollock ML: Generalized equations for predicting body density of men. Br J Nutr. 1978, 40: 497-504. 10.1079/BJN19780152.CrossRefPubMed Jackson AS, Pollock ML: Generalized equations for predicting body density of men. Br J Nutr. 1978, 40: 497-504. 10.1079/BJN19780152.CrossRefPubMed
18.
go back to reference Ohtani M, Sugita M, Maruyama K: Amino acid mixture improves training efficiency in athletes. J Nutr. 2006, 136: 538S-543S.PubMed Ohtani M, Sugita M, Maruyama K: Amino acid mixture improves training efficiency in athletes. J Nutr. 2006, 136: 538S-543S.PubMed
19.
go back to reference Bijur PE, Silver W, Gallagher EJ: Reliability of the visual analog scale for measurement of acute pain. Acad Emerg Med. 2001, 8: 1153-1157. 10.1111/j.1553-2712.2001.tb01132.x.CrossRefPubMed Bijur PE, Silver W, Gallagher EJ: Reliability of the visual analog scale for measurement of acute pain. Acad Emerg Med. 2001, 8: 1153-1157. 10.1111/j.1553-2712.2001.tb01132.x.CrossRefPubMed
20.
go back to reference Brown SJ, Child RB, Day SH, Donnelly AE: Exercise-induced skeletal muscle damage and adaptation following repeated bouts of eccentric muscle contractions. J Sports Sci. 1997, 15: 215-222. 10.1080/026404197367498.CrossRefPubMed Brown SJ, Child RB, Day SH, Donnelly AE: Exercise-induced skeletal muscle damage and adaptation following repeated bouts of eccentric muscle contractions. J Sports Sci. 1997, 15: 215-222. 10.1080/026404197367498.CrossRefPubMed
21.
go back to reference Callaghan MJ, McCarthy CJ, Al-Omar A, Oldham JA: The reproducibility of multi-joint isokinetic and isometric assessments in a healthy and patient population. Clin Biomech (Bristol, Avon). 2000, 15: 678-683. 10.1016/S0268-0033(00)00032-2.CrossRef Callaghan MJ, McCarthy CJ, Al-Omar A, Oldham JA: The reproducibility of multi-joint isokinetic and isometric assessments in a healthy and patient population. Clin Biomech (Bristol, Avon). 2000, 15: 678-683. 10.1016/S0268-0033(00)00032-2.CrossRef
22.
go back to reference Dangin M, Boirie Y, Garcia-Rodenas C, Gachon P, Fauquant J, Callier P, Ballevre O, Beaufrere B: The digestion rate of protein is an independent regulating factor of postprandial protein retention. Am J Physiol Endocrinol Metab. 2001, 280: E340-348.PubMed Dangin M, Boirie Y, Garcia-Rodenas C, Gachon P, Fauquant J, Callier P, Ballevre O, Beaufrere B: The digestion rate of protein is an independent regulating factor of postprandial protein retention. Am J Physiol Endocrinol Metab. 2001, 280: E340-348.PubMed
23.
go back to reference Lee J, Clarkson PM: Plasma creatine kinase activity and glutathione after eccentric exercise. Med Sci Sports Exerc. 2003, 35: 930-936. 10.1249/01.MSS.0000069553.47739.36.CrossRefPubMed Lee J, Clarkson PM: Plasma creatine kinase activity and glutathione after eccentric exercise. Med Sci Sports Exerc. 2003, 35: 930-936. 10.1249/01.MSS.0000069553.47739.36.CrossRefPubMed
24.
go back to reference Sayers SP, Clarkson PM: Short-term immobilization after eccentric exercise. Part II: creatine kinase and myoglobin. Med Sci Sports Exerc. 2003, 35: 762-768. 10.1249/01.MSS.0000064933.43824.ED.CrossRefPubMed Sayers SP, Clarkson PM: Short-term immobilization after eccentric exercise. Part II: creatine kinase and myoglobin. Med Sci Sports Exerc. 2003, 35: 762-768. 10.1249/01.MSS.0000064933.43824.ED.CrossRefPubMed
25.
go back to reference Proske U, Allen TJ: Damage to skeletal muscle from eccentric exercise. Exerc Sport Sci Rev. 2005, 33: 98-104. 10.1097/00003677-200504000-00007.CrossRefPubMed Proske U, Allen TJ: Damage to skeletal muscle from eccentric exercise. Exerc Sport Sci Rev. 2005, 33: 98-104. 10.1097/00003677-200504000-00007.CrossRefPubMed
26.
go back to reference Tipton KD, Elliott TA, Cree MG, Aarsland AA, Sanford AP, Wolfe RR: Stimulation of net muscle protein synthesis by whey protein ingestion before and after exercise. Am J Physiol Endocrinol Metab. 2007, 292: E71-76. 10.1152/ajpendo.00166.2006.CrossRefPubMed Tipton KD, Elliott TA, Cree MG, Aarsland AA, Sanford AP, Wolfe RR: Stimulation of net muscle protein synthesis by whey protein ingestion before and after exercise. Am J Physiol Endocrinol Metab. 2007, 292: E71-76. 10.1152/ajpendo.00166.2006.CrossRefPubMed
27.
go back to reference Tipton KD, Rasmussen BB, Miller SL, Wolf SE, Owens-Stovall SK, Petrini BE, Wolfe RR: Timing of amino acid-carbohydrate ingestion alters anabolic response of muscle to resistance exercise. Am J Physiol Endocrinol Metab. 2001, 281: E197-206.PubMed Tipton KD, Rasmussen BB, Miller SL, Wolf SE, Owens-Stovall SK, Petrini BE, Wolfe RR: Timing of amino acid-carbohydrate ingestion alters anabolic response of muscle to resistance exercise. Am J Physiol Endocrinol Metab. 2001, 281: E197-206.PubMed
Metadata
Title
Effect of carbohydrate-protein supplement timing on acute exercise-induced muscle damage
Authors
James P White
Jacob M Wilson
Krista G Austin
Beau K Greer
Noah St John
Lynn B Panton
Publication date
01-12-2008
Publisher
BioMed Central
DOI
https://doi.org/10.1186/1550-2783-5-5

Other articles of this Issue 1/2008

Journal of the International Society of Sports Nutrition 1/2008 Go to the issue