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Journal of the International Society of Sports Nutrition

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Open Access 01-12-2013 | Research article

Co-ingestion of carbohydrate and whey protein isolates enhance PGC-1α mRNA expression: a randomised, single blind, cross over study

Authors: Karen M Hill, Christos G Stathis, Esther Grinfeld, Alan Hayes, Andrew J McAinch

Published in: Journal of the International Society of Sports Nutrition | Issue 1/2013

Abstract

Background

Whey protein isolates (WPI) supplementation is known to improve resistance training adaptations. However, limited information is available on the effects of WPI plus carbohydrate (CHO) supplementation on endurance training adaptations.

Method

Six endurance trained male cyclists and triathletes (age 29 ± 4 years, weight 74 ± 2 kg, VO_{2 max} 63 ± 3 ml oxygen^. kg^-1. Min^-1, height 183 ± 5 cm; mean ± SEM) were randomly assigned to one of two dietary interventions in a single blind cross over design; CHO or CHO + WPI. Each dietary intervention was followed for 16 days which included the last 2 days having increased CHO content, representing a CHO loading phase. The dietary interventions were iso-caloric and carbohydrate content matched. On completion of the dietary intervention, participants performed an exercise bout, consisting of cycling for 60 min at 70% VO_{2 max,} followed by time trial to exhaustion at 90% VO_{2 max} and recovered in the laboratory for 6 hours. Blood samples and muscle biopsies were taken at various time points at rest and through the exercise trial and recovery.

Results

Compared to CHO, CHO + WPI increased plasma insulin during recovery at 180 mins (P < 0.05) and peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) mRNA expression at the end of 6 hours of recovery (P < 0.05). Muscle glycogen did not differ between the two trials.

Conclusion

This study showed co-ingestion of CHO + WPI may have beneficial effects on recovery and adaptations to endurance exercise via, increased insulin response and up regulation of PGC-1α mRNA expression.

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Rodriguez N, Vislocky L, Gaine P: Dietary protein, endurance exercise, and human skeletal-musvercle protein turn. Curr Opin Clin Nutr. 2007, 10: 40-45. 10.1097/MCO.0b013e3280115e3b.CrossRef

Hawley J, Tipton K, Millard-Stafford M: Promoting training adaptations through nutritional interventions. J Sport Sci. 2006, 24 (7): 709-721. 10.1080/02640410500482727.CrossRef

Ivy J: Regulation of muscle glycogen repletion, muscle protein synthesis and repair following exercise. J Sports Sci Med. 2004, 3: 131-138. 2004PubMedCentralPubMed

Ha E, Zemel M: Functional properties of whey, whey components, and essential amino acids: mechanisms underlying health benefits for acticve people. J Nutr Biochem. 2003, 14: 251-258. 10.1016/S0955-2863(03)00030-5.CrossRefPubMed

Cox GR, Clark SA, Cox AJ, Halson SL, Hargreaves M, Hawley JA, Jeacocke N, Snow RJ, Yeo WK, Burke LM: Daily training with high carbohydrate availability increases exogenous carbohydrate oxidation during endurance cycling. J Appl Physiol. 2010, 109 (1): 126-134. 10.1152/japplphysiol.00950.2009.CrossRefPubMed

Rowlands D, Thorp R, Rossler K, Graham D, Rockell M: Efect of protein-rich feeding on recovery after intense exercise. Int J Sport Nutr Exerc Metab. 2007, 17: 521-543.PubMed

Jentjens R, Jeukendrup A: Determinants of post exericse glycogen synthesis during short term recovery. Sports Med. 2003, 33 (2): 117-144. 10.2165/00007256-200333020-00004.CrossRefPubMed

Rauch H, Gibson A, Lambert E, Noakes T: A signalling role for muscle glycogen in the regulation of pace during prolonged exercise. Brit J Sport Med. 2005, 39: 34-38. 10.1136/bjsm.2003.010645.CrossRef

Westerblad H, Bruton JD, Katz A: Skeletal muscle: energy metabolism, fiber types, fatigue and adaptability. Exp Cell Res. 2010, 316 (18): 3093-3099. 10.1016/j.yexcr.2010.05.019.CrossRefPubMed

10.

Kerksick C, Harvey T, Stout J, Campbell B, Wilborn C, Kreider R, Kalman D, Ziegenfuss T, Lopez H, Landis J, Ivy JL, Antonio J: International Society of Sports Nutrition position stand: nutrient timing. J Int Soc Sports Nutr. 2008, 5: 17-10.1186/1550-2783-5-17.PubMedCentralCrossRefPubMed

11.

Maughan R: Nutritional status, metabolic responses to exercise and implications for performance. Biochem Soc. 2003, 31 (6): 1267-1269. 10.1042/BST0311267.CrossRef

12.

Hawley JA, Burke LM, Phillips SM, Spriet LL: Nutritional modulation of training-induced skeletal muscle adaptations. J Appl Physiol. 2011, 110 (3): 834-845. 10.1152/japplphysiol.00949.2010.CrossRefPubMed

13.

Smith T, Montain S, Anderson D, Young A: Plasma amino acid responses after consumption of beverages with varying protein type. Int J Sport Nutr Exerc Metab. 2009, 19: 1-17.PubMed

14.

Tang JE, Moore DR, Kujbida GW, Tarnopolsky MA, Phillips SM: Ingestion of whey hydrolysate, casein, or soy protein isolate: effects on mixed muscle protein synthesis at rest and following resistance exercise in young men. J Appl Physiol. 2009, 107 (3): 987-992. 10.1152/japplphysiol.00076.2009.CrossRefPubMed

15.

Tipton K, Wolfe R: Protein and amino acids for athletes. J Sport Sci. 2004, 22: 65-79. 10.1080/0264041031000140554.CrossRef

16.

Wilkinson SB, Phillips SM, Atherton PJ, Patel R, Yarasheski KE, Tarnopolsky MA, Rennie MJ: Differential effects of resistance and endurance exercise in the fed state on signalling molecule phosphorylation and protein synthesis in human muscle. J Physiol. 2008, 586 (Pt 15): 3701-3717.PubMedCentralCrossRefPubMed

17.

Camera DM, Edge J, Short MJ, Hawley JA, Coffey VG: Early time course of Akt phosphorylation after endurance and resistance exercise. Med Sci Sports Exerc. 2010, 42 (10): 1843-1852. 10.1249/MSS.0b013e3181d964e4.CrossRefPubMed

18.

Walker D, Dickinson J, Timmerman K, Drummond M, Reidy P, Fry C, Gundermann D, Rasmussen B: Exercise, amino acids, and ageing in the control of human muscle protein synthesis. Med Sci Sports Exerc. 2011, published ahead of Print

19.

Cribb PJ, Williams AD, Carey MF, Hayes A: The effect of whey isolate and resistance training on strength, body composition, and plasma glutamine. Int J Sport Nutr Exerc Metab. 2006, 16 (5): 494-509.PubMed

20.

Hulmi JJ, Lockwood CM, Stout JR: Effect of protein/essential amino acids and resistance training on skeletal muscle hypertrophy: A case for whey protein. Nutr Metab (Lond). 2010, 7: 51-10.1186/1743-7075-7-51.CrossRef

21.

Jentjens RL, van Loon LJ, Mann CH, Wagenmakers AJ, Jeukendrup AE: Addition of protein and amino acids to carbohydrates does not enhance postexercise muscle glycogen synthesis. J Appl Physiol. 2001, 91 (2): 839-846.PubMed

22.

Evans WJ, Phinney SD, Young VR: Suction applied to a muscle biopsy maximizes sample size. Med Sci Sports Exerc. 1982, 14 (1): 101-102.PubMed

23.

Lowry O, Passonneau J: A flexible system of enzymatic analysis. 1972, New York: Academic

24.

Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 2001, 25 (4): 402-408. 10.1006/meth.2001.1262.CrossRefPubMed

25.

Madsen K, Pedersen PK, Rose P, Richter EA: Carbohydrate supercompensation and muscle glycogen utilization during exhaustive running in highly trained athletes. Eur J Appl Physiol Occup Phys. 1990, 61 (5–6): 467-472.CrossRef

26.

Ivy J, Goforth HJ, Damon B, McCauley T, Parsons E, Price T: Early post exercise muscle glycogen recovery is enhanced with a carbohydrate-protein supplement. J Appl Physiol. 2002, 93: 1337-1344.CrossRefPubMed

27.

Van Loon L, Saris W, Kruijshoop M, Wagenmeakers A: Maximising postexercise muscle glycogen synthesis: carbohydrate supplementation and the aplication of amino acid or protein hydrolysate mixtures. Am J Clin Nutr. 2000, 72: 106-111.PubMed

28.

Zawadzki K, Yaspelkis B, Ivy J: Carbohydrate-protein complex increases the rate of muscle glycogen storage after exercise. J Appl Physiol. 1992, 72: 1854-1859.PubMed

29.

Nilsson M, Holst J, Bjorck I: Metabolic effect of amino acid mixtures and whey protein in healthy subjects: studis using glucose equivalent drinks. Am J Clin Nutr. 2007, 85: 996-1004.PubMed

30.

Power O, Hallihan A, Jakeman P: Human insulinotropic response to oral ingestion of native and hydrolysed whey protein. Amino Acids. 2009, 37: 333-339. 10.1007/s00726-008-0156-0.CrossRefPubMed

31.

Claessens M, Saris W, Van Baak M: Glucagon ad insulin responses after ingestion of different amounts of intact and hydrolysed proteins. Brit J Nutr. 2008, 100: 61-69.CrossRefPubMed

32.

Van Loon L, Saris W, Verhagen H, Wagenmakers A: PLasma insulin responses following the ingestion of different amino acid/protein carbohydrate mixtures. Am J Clin Nutr. 2000, 72: 96-105.PubMed

33.

Rowlands DS, Thomson JS, Timmons BW, Raymond F, Fuerholz A, Mansourian R, Zwahlen MC, Metairon S, Glover E, Stellingwerff T, Kussmann M, Tarnopolsky MA: Transcriptome and translational signaling following endurance exercise in trained skeletal muscle: impact of dietary protein. Physiol Genomics. 2011, 43 (17): 1004-1020. 10.1152/physiolgenomics.00073.2011.CrossRefPubMed

34.

Morrison PJ, Hara D, Ding Z, Ivy JL: Adding protein to a carbohydrate supplement provided after endurance exercise enhances 4E-BP1 and RPS6 signaling in skeletal muscle. J Appl Physiol. 2008, 104 (4): 1029-1036. 10.1152/japplphysiol.01173.2007.CrossRefPubMed

35.

Cunningham JT, Rodgers JT, Arlow DH, Vazquez F, Mootha VK, Puigserver P: mTOR controls mitochondrial oxidative function through a YY1-PGC-1alpha transcriptional complex. Nature. 2007, 450 (7170): 736-740. 10.1038/nature06322.CrossRefPubMed

36.

Hood DA: Mechanisms of exercise-induced mitochondrial biogenesis in skeletal muscle. Appl Physiol Nutr Metab. 2009, 34 (3): 465-472. 10.1139/H09-045.CrossRefPubMed

37.

Lin J, Handschin C, Spiegelman BM: Metabolic control through the PGC-1 family of transcription coactivators. Cell Metab. 2005, 1 (6): 361-370. 10.1016/j.cmet.2005.05.004.CrossRefPubMed

38.

Irrcher I, Adhihetty PJ, Joseph AM, Ljubicic V, Hood DA: Regulation of mitochondrial biogenesis in muscle by endurance exercise. Sports Med. 2003, 33 (11): 783-793. 10.2165/00007256-200333110-00001.CrossRefPubMed

39.

Koulmann N, Bigard AX: Interaction between signalling pathways involved in skeletal muscle responses to endurance exercise. Pflugers Arch. 2006, 452 (2): 125-139. 10.1007/s00424-005-0030-9.CrossRefPubMed

40.

Akimoto T, Pohnert SC, Li P, Zhang M, Gumbs C, Rosenberg PB, Williams RS, Yan Z: Exercise stimulates Pgc-1alpha transcription in skeletal muscle through activation of the p38 MAPK pathway. J Biol Chem. 2005, 280 (20): 19587-19593. 10.1074/jbc.M408862200.CrossRefPubMed

41.

Baar K, Wende AR, Jones TE, Marison M, Nolte LA, Chen M, Kelly DP, Holloszy JO: Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1. FASEB J. 2002, 16 (14): 1879-1886. 10.1096/fj.02-0367com.CrossRefPubMed

42.

Koopman R, Pannemans D, Jeukendrup A, Gijsen A, Senden J, Halliday D, Saris W, Van Loon L, Wagenmakers A: Combined ingestion of protein and carbohydrate improves protein balance. Am J Physiol Endocrinol and Metab. 2004, 287: E712-E720. 10.1152/ajpendo.00543.2003.CrossRef

43.

Beelen M, Zorenc A, Pennings B, Senden J, Kuipers H, Van Loon L: Impact of protein coingestion on muscle protein synthesis during continuous endurance type exercise. Am J Physiol Endocrinol and Metab. 2011, 300: E945-E954. 10.1152/ajpendo.00446.2010.CrossRef

44.

Breen L, Philp A, Witard OC, Jackman SR, Selby A, Smith K, Baar K, Tipton KD: The influence of carbohydrate-protein co-ingestion following endurance exercise on myofibrillar and mitochondrial protein synthesis. J Physiol. 2011, 589 (Pt 16): 4011-4025.PubMedCentralCrossRefPubMed

45.

Rodriguez NR, Di Marco NM, Langley S: American College of Sports Medicine position stand. Nutrition and athletic performance. Med Sci Sports Exerc. 2009, 41 (3): 709-731. 10.1249/MSS.0b013e31890eb86.CrossRefPubMed

Title: Co-ingestion of carbohydrate and whey protein isolates enhance PGC-1α mRNA expression: a randomised, single blind, cross over study
Authors: Karen M Hill
Christos G Stathis
Esther Grinfeld
Alan Hayes
Andrew J McAinch
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: Journal of the International Society of Sports Nutrition / Issue 1/2013
Electronic ISSN: 1550-2783
DOI: https://doi.org/10.1186/1550-2783-10-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Co-ingestion of carbohydrate and whey protein isolates enhance PGC-1α mRNA expression: a randomised, single blind, cross over study

Abstract

Background

Method

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Method

Results

Conclusion

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