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Open Access 01-12-2010 | Research

CCL20/CCR6 expression profile in pancreatic cancer

Authors: Claudia Rubie, Vilma Oliveira Frick, Pirus Ghadjar, Mathias Wagner, Henner Grimm, Benjamin Vicinus, Christoph Justinger, Stefan Graeber, Martin K Schilling

Published in: Journal of Translational Medicine | Issue 1/2010

Abstract

Background

CCL20 and its receptor CCR6 have been shown to play a role in the onset, development and metastatic spread of various gastrointestinal malignancies. In this study, the expression profile and clinical significance of the CCL20/CCR6 system in distinct benign, pre-malignant and malignant pancreatic tissues was investigated.

Methods

Using RealTime-PCR, enzyme-linked immunosorbent assay (ELISA), Western Blot and immunohistochemistry, we have analyzed the expression profile of CCL20/CCR6 in resection specimens from patients with chronic pancreatitis (CP) (n = 22), pancreatic cystadenoma (PA) (n = 11) and pancreatic carcinoma (PCA) (n = 25) as well as in the respective matched normal pancreatic tissues.

Results

CCL20 mRNA and protein was weakly expressed in normal pancreatic tissues and CP and PA specimens but significantly up-regulated in PCA (8-fold) as compared to the matched normal tissue (P < 0.05). Moreover, CCL20 mRNA and protein expression was significantly associated with advanced T-category in patients with PCA (P < 0.05). CCR6 mRNA showed a significant up-regulation in all three disease entities as compared to normal tissues (P < 0.05, respectively).

Conclusion

CCL20 and CCR6 were significantly up-regulated in PCA as compared to the normal pancreatic tissue and CCL20 was significantly associated with advanced T-category in PCA patients. This suggests that CCL20 and CCR6 play a role in the development and progression of PCA and may constitute potential targets for novel treatment strategies.

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Title: CCL20/CCR6 expression profile in pancreatic cancer
Authors: Claudia Rubie
Vilma Oliveira Frick
Pirus Ghadjar
Mathias Wagner
Henner Grimm
Benjamin Vicinus
Christoph Justinger
Stefan Graeber
Martin K Schilling
Publication date: 01-12-2010
Publisher: BioMed Central
Published in: Journal of Translational Medicine / Issue 1/2010
Electronic ISSN: 1479-5876
DOI: https://doi.org/10.1186/1479-5876-8-45

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