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Open Access 01-12-2013 | Research

Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer

Authors: Dong-liang Chen, Zhi-qiang Wang, Chao Ren, Zhao-lei Zeng, De-shen Wang, Hui-yan Luo, Feng Wang, Miao-zhen Qiu, Long Bai, Dong-sheng Zhang, Feng-hua Wang, Yu-hong Li, Rui-hua Xu

Published in: Journal of Translational Medicine | Issue 1/2013

Abstract

Background

Paxillin (PXN) has been found to be aberrantly regulated in various malignancies and involved in tumor growth and invasion. The clinicopathological and prognostic significance of PXN in gastric cancer is still unclear.

Methods

The expression of PXN was determined in paired gastric cancer tissues and adjacent normal tissues by Western blotting and real-time PCR. Immunohistochemistry was performed to detect the expression of PXN in 239 gastric cancer patients. Statistical analysis was applied to investigate the correlation between PXN expression and clinicopathological characteristics and prognosis in patients. Additionally, the effects of PXN on gastric cancer cell proliferation and migration were also evaluated.

Results

PXN was up-regulated in gastric cancer tissues and cell lines as compared with adjacent normal tissues and normal gastric epithelial cell line GES-1. Overexpression of PXN was correlated with distant metastasis (P = 0.001) and advanced tumor stage (P = 0.021) in gastric cancer patients. Patients with high PXN expression tended to have poor prognosis compared with patients with low PXN expression (P < 0.001). Multivariate analysis demonstrated that PXN expression was an independent prognostic factor (P = 0.020). Moreover, ectopic expression of PXN promotes cell proliferation and migration in AGS cells whereas knockdown of PXN inhibits cell proliferation and migration in SGC7901 cells.

Conclusions

PXN plays an important role in tumor progression and may be used as a potential prognostic indicator in gastric cancer.

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Title: Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer
Authors: Dong-liang Chen
Zhi-qiang Wang
Chao Ren
Zhao-lei Zeng
De-shen Wang
Hui-yan Luo
Feng Wang
Miao-zhen Qiu
Long Bai
Dong-sheng Zhang
Feng-hua Wang
Yu-hong Li
Rui-hua Xu
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: Journal of Translational Medicine / Issue 1/2013
Electronic ISSN: 1479-5876
DOI: https://doi.org/10.1186/1479-5876-11-277

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Abstract

Background

Methods

Results

Conclusions

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