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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2006

Open Access 01-12-2006 | Research

Identification of differential gene expression in in vitro FSH treated pig granulosa cells using suppression subtractive hybridization

Authors: A Bonnet, PO Frappart, P Dehais, G Tosser-Klopp, F Hatey

Published in: Reproductive Biology and Endocrinology | Issue 1/2006

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Abstract

FSH, which binds to specific receptors on granulosa cells in mammals, plays a key role in folliculogenesis. Its biological activity involves stimulation of intercellular communication and upregulation of steroidogenesis, but the entire spectrum of the genes regulated by FSH has yet to be fully characterized.
In order to find new regulated transcripts, however rare, we have used a Suppression Subtractive Hybridization approach (SSH) on pig granulosa cells in primary culture treated or not with FSH. Two SSH libraries were generated and 76 clones were sequenced after selection by differential screening. Sixty four different sequences were identified, including 3 novel sequences. Experiments demonstrated the presence of 25 regulated transcripts.
A gene ontology analysis of these 25 genes revealed (1) catalytic; (2) transport; (3) signal transducer; (4) binding; (5) anti-oxidant and (6) structural activities. These findings may deepen our understanding of FSH's effects. Particularly, they suggest that FSH is involved in the modulation of peroxidase activity and remodelling of chromatin.
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Metadata
Title
Identification of differential gene expression in in vitro FSH treated pig granulosa cells using suppression subtractive hybridization
Authors
A Bonnet
PO Frappart
P Dehais
G Tosser-Klopp
F Hatey
Publication date
01-12-2006
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2006
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-4-35

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