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Malaria Journal
Published in: Malaria Journal 1/2004

Open Access 01-12-2004 | Methodology

A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real-time PCR

Authors: Anne Purfield, Amy Nelson, Anita Laoboonchai, Kanungnij Congpuong, Phillip McDaniel, R Scott Miller, Kathy Welch, Chansuda Wongsrichanalai, Steven R Meshnick

Published in: Malaria Journal | Issue 1/2004

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Abstract

Background

Surveillance for drug-resistant Plasmodium falciparum should be a component of malaria control programmes. Real-time PCR methods for the detection of parasite single-nucleotide polymorphisms (SNPs) and gene amplification could be useful survellance tools.

Methods

A real-time PCR assay has been developed that identifies single nucleotide polymorphisms (SNPs) at amino acids 86, 184, 1034 and 1042 in the P. falciparum multi-drug resistant (pfmdr 1) gene that may be associated with anti-malarial drug resistance.

Results

This assay has a sensitivity and specificity of 94% and 100% when compared to traditional PCR methods for genotyping. Only 54 of 68 (79%) paired pre- and post-culture DNA samples were concordant at all four loci.

Conclusion

Real-time PCR is a sensitive and specific method to detect SNP's in pfmdr 1. Genotypes of parasites after in vitro culture may not reflect that seen in vivo.
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Metadata
Title
A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real-time PCR
Authors
Anne Purfield
Amy Nelson
Anita Laoboonchai
Kanungnij Congpuong
Phillip McDaniel
R Scott Miller
Kathy Welch
Chansuda Wongsrichanalai
Steven R Meshnick
Publication date
01-12-2004
Publisher
BioMed Central
Published in
Malaria Journal / Issue 1/2004
Electronic ISSN: 1475-2875
DOI
https://doi.org/10.1186/1475-2875-3-9

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