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Malaria Journal
Published in: Malaria Journal 1/2013

Open Access 01-12-2013 | Research

Immunogenicity of bacterial-expressed recombinant Plasmodium knowlesi merozoite surface protein-142 (MSP-142)

Authors: Fei Wen Cheong, Mun Yik Fong, Yee Ling Lau, Rohela Mahmud

Published in: Malaria Journal | Issue 1/2013

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Abstract

Background

Plasmodium knowlesi is the fifth Plasmodium species that can infect humans. The Plasmodium merozoite surface protein-142 (MSP-142) is a potential candidate for malaria vaccine. However, limited studies have focused on P. knowlesi MSP-142.

Methods

A ~42 kDa recombinant P. knowlesi MSP-142 (pkMSP-142) was expressed using an Escherichia coli system. The purified pkMSP-142 was evaluated with malaria and non-malaria human patient sera (n = 189) using Western blots and ELISA. The immunogenicity of pkMSP-142 was evaluated in mouse model.

Results

The purified pkMSP-142 had a sensitivity of 91.0% for detection of human malaria in both assays. Specificity was 97.5 and 92.6% in Western blots and ELISA, respectively. Levels of cytokine interferon-gamma, interleukin-2, interleukin-4, and interleukin-10 significantly increased in pkMSP-142-immunized mice as compared to the negative control mice. pkMSP-142-raised antibody had high endpoint titres, and the IgG isotype distribution was IgG1 > IgG2b > IgG3 > IgG2a.

Conclusions

pkMSP-142 was highly immunogenic and able to detect human malaria. Hence, pkMSP-142 would be a useful candidate for malaria vaccine development and seroprevalence studies.
Appendix
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Metadata
Title
Immunogenicity of bacterial-expressed recombinant Plasmodium knowlesi merozoite surface protein-142 (MSP-142)
Authors
Fei Wen Cheong
Mun Yik Fong
Yee Ling Lau
Rohela Mahmud
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Malaria Journal / Issue 1/2013
Electronic ISSN: 1475-2875
DOI
https://doi.org/10.1186/1475-2875-12-454

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WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

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