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Cancer Cell International
Published in: Cancer Cell International 1/2014

Open Access 01-12-2014 | Primary research

Establishment and characterization of two primary breast cancer cell lines from young Indian breast cancer patients: mutation analysis

Authors: Santhi Latha Pandrangi, Sarangadhara Appala Raju Bagadi, Navin Kumar Sinha, Manoj Kumar, Rima Dada, Meena Lakhanpal, Abha Soni, Shreshtha Malvia, Sheeba Simon, Chintamani Chintamani, Ravindar Singh Mohil, Dinesh Bhatnagar, Sunita Saxena

Published in: Cancer Cell International | Issue 1/2014

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Abstract

Two novel triple negative breast cancer cell lines, NIPBC-1 and NIPBC-2 were successfully established from primary tumors of two young breast cancer patients aged 39 and 38 years respectively, diagnosed as infiltrating duct carcinoma of breast. Characterization of these cell lines showed luminal origin with expression of epithelial specific antigen and cytokeratin 18 and presence of microfilaments and secretary vesicles, microvilli, tight junctions and desmosomes on ultra-structural analysis. Both the cell lines showed anchorage independent growth and invasion of matrigel coated membranes. Karyotype analysis showed aneuploidy, deletions and multiple rearrangements in chromosomes 7, 9, X and 11 and isochromosomes 17q in both the cell lines. P53 mutational analysis revealed no mutation in the coding region in both the cell lines; however NIPBC-2 cell line showed presence of heterozygous C/G polymorphism, g.417 C > G (NM_000546.5) resulting in Arg/Pro allele at codon 72 of exon 4. Screening for mutations in BRCA1&2 genes revealed presence of three heterozygous polymorphisms in exon 11 of BRCA1 and 2 polymorphisms in exons 11, and14 of BRCA2 gene in both the cell lines. Both the cell lines showed presence of CD 44+/24-breast cancer stem cells and capability of producing mammosphere on culture. The two triple negative breast cancer cell lines established from early onset breast tumors can serve as novel invitro models to study mechanisms underlying breast tumorigenesis in younger age group patients and also identification of new therapeutic modalities targeting cancer stem cells.
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Metadata
Title
Establishment and characterization of two primary breast cancer cell lines from young Indian breast cancer patients: mutation analysis
Authors
Santhi Latha Pandrangi
Sarangadhara Appala Raju Bagadi
Navin Kumar Sinha
Manoj Kumar
Rima Dada
Meena Lakhanpal
Abha Soni
Shreshtha Malvia
Sheeba Simon
Chintamani Chintamani
Ravindar Singh Mohil
Dinesh Bhatnagar
Sunita Saxena
Publication date
01-12-2014
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2014
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/1475-2867-14-14

Other articles of this Issue 1/2014

Cancer Cell International 1/2014 Go to the issue

Primary research

Anti-tumor effect of a novel PI3-kinase inhibitor, SF1126, in 12 V-Ha-Ras transgenic mouse glioma model

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The differential susceptibilities of MCF-7 and MDA-MB-231 cells to the cytotoxic effects of curcumin are associated with the PI3K/Akt-SKP2-Cip/Kips pathway

Primary research

Epigenetic alteration by DNA-demethylating treatment restores apoptotic response to glucocorticoids in dexamethasone-resistant human malignant lymphoid cells

Primary research

Establishment and characterization of a novel primary hepatocellular carcinoma cell line with metastatic ability in vivo

Primary research

Hypericin-mediated photodynamic therapy induces apoptosis of myoloma SP2/0 cells depended on caspase activity in vitro

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Estrogen mediated expression of nucleophosmin 1 in human endometrial carcinoma clinical stages through estrogen receptor-α signaling
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