Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2014

Open Access 01-12-2014 | Research article

Organic silicon protects human neuroblastoma SH-SY5Y cells against hydrogen peroxide effects

Authors: Alba Garcimartín, José J Merino, Maria Pilar González, Maria Isabel Sánchez-Reus, Francisco J Sánchez-Muniz, Sara Bastida, Juana Benedí

Published in: BMC Complementary Medicine and Therapies | Issue 1/2014

Login to get access

Abstract

Background

Hydrogen peroxide (H2O2) is a toxic agent that induces oxidative stress and cell death. Silicon (Si) is a biological element involved in limiting aluminium (Al) absorption with possible preventive effects in Alzheimer’s disease. However, Si has not yet been associated with other neuroprotective mechanisms.

Methods

The present experiments evaluated in the SH-SY5Y human neuroblastoma cell line the possible role of different Si G5 (50-1000 ng/mL) concentrations in preventing cellular death induced by H2O2 (400 μM, 24 hours).

Results

Our findings showed that H2O2 promoted cell death in the human SH-SY5Y cell cultures and this could be prevented by Si treatment. The loss in cell viability mediated by H2O2 was due to an apoptotic and necrotic process. Apoptotic death was incurred by regulating caspase-8 activity in the extrinsic pathway. The apoptotic and necrotic cell death induced by H2O2 was almost totally reversed by Si (50-500 ng/mL), indicating that it down-regulates both processes in H2O2 treated cells.

Conclusions

According to our data, Si is able to increase SH-SY5Y cell survival throughout partially blocking cellular damage related to oxidative stress through a mechanism that would affect H2O2/ROS elimination.
Appendix
Available only for authorised users
Literature
1.
Edwardson JA, Moore PB, Ferrier IN, Lilley JS, Newton GW, Barker J, Templar J, Day JP: Effect of silicon on gastrointestinal absorption of aluminum. Lancet. 1993, 342: 211-212. 10.1016/0140-6736(93)92301-9.CrossRefPubMed
2.
Carlisle EM, Curran MJ: Effect of dietary silicon and aluminum on silicon and aluminum levels in rat brain. Alzheimer Dis Assoc Disord. 1987, 1: 83-89. 10.1097/00002093-198701020-00003.CrossRefPubMed
3.
Bellès M, Sanchez DJ, Gómez M, Corbella J, Domingo JL: Silicon reduces aluminum accumulation in rats: relevance to the aluminum hypothesis of Alzheimer’s disease. Alzheimer Dis Assoc Disord. 1998, 12: 83-87. 10.1097/00002093-199806000-00005.CrossRefPubMed
4.
Greenwood NN, Earnshaw A: Chemistry of the Elements. 1997, Oxford: Butterworth-Heinemann, 2
5.
Carlisle EM: Silicon: an essential element for the chick. Science. 1972, 178: 619-621. 10.1126/science.178.4061.619.CrossRefPubMed
6.
Schwarz K, Milne DB: Growth promoting effects of silicon in rats. Nature. 1972, 239: 333-334. 10.1038/239333a0.CrossRefPubMed
7.
Candy JM, Edwardson JA, Klinowski J, Oakley AE, Perry EK, Perry RH: Colocalisation of Aluminum and Silicon in Senile Plaques: Implications for the Neurochemical Pathology of Alzheimer’s Disease. Senile Dementia of the Alzheimer Type. Edited by: Traber J, Gispen WH. 1985, Heidelberg: Springer, 183-197.CrossRef
8.
Jurkić LM, Cepanec I, Pavelić KS, Pavelić K: Biological and therapeutic effects of ortho-silicic acid and some ortho-silicic acid-releasing compounds: new perspectives for therapy. Nutr Metab. 2013, 10: 1-12. 10.1186/1743-7075-10-1.CrossRef
9.
Dobbie JW, Smith MJB: The silicon content of body fluids. Scott Med J. 1982, 27: 17-19.PubMed
10.
Dobbie JW, Smith MJB: Silicon: its role in medicine and biology. Scott Med J. 1982, 27: 1-2.PubMed
11.
D’Haese PC, Shaheen FA, Huraib SO, Djukanovic L, Polenakovic MH, Spasovski G, Shikole A, Schurgers ML, Daneels RF, Lamberts LV, Van Landeghem GF, De Broe ME: Increased silicon levels in dialysis patients due to high silicon content in the drinking water, inadequate water treatment procedures, and concentrate contamination: a multicentre study. Nephrol Dial Transplant. 1995, 10: 1838-1844.PubMed
12.
Perry CC, Keeling-Tucker T: Aspects of the bioinorganic chemistry of silicon in conjunction with the biometals calcium, iron and aluminium. J Inorg Biochem. 1998, 69: 181-191. 10.1016/S0162-0134(97)10017-4.CrossRefPubMed
13.
Exley C: Darwin, natural selection and the biological essentiality of aluminium and silicon. Trends Biochem Sci. 2009, 34: 589-593. 10.1016/j.tibs.2009.07.006.CrossRefPubMed
14.
Scientific Committee for Food: Reports of the Scientific Committee for Food (Thirty-first series). Nutrient and Energy Intakes for the European Community. 1993, Luxembourg: European Commission
15.
European Food Safety Authority: Opinion of the scientific panel on dietetic products, nutrition and allergies on a request from the commission related to the tolerable upper intake level of silicon. EFSA J. 2004, 60: 1-11.
16.
Van Dyck K, Robberecht H, Van Cauwenbergh R, Van Vlaslaer V, Deelstra H: Indication of silicon essentiality in humans. serum concentrations in Belgian children and adults including pregnant women. Biol Trace Elem Res. 2000, 77: 25-32. 10.1385/BTER:77:1:25.CrossRefPubMed
17.
Bissé E, Epting T, Beil A, Lindinger G, Lang H, Wieland H: Reference values for serum silicon in adults. Anal Biochem. 2005, 337: 130-135. 10.1016/j.ab.2004.10.034.CrossRefPubMed
18.
González-Muñoz MJ, Meseguer I, Sanchez-Reus MI, Schultz A, Olivero R, Benedi J, Sánchez-Muniz FJ: Beer consumption reduces cerebral oxidation caused by aluminum toxicity by normalizing gene expresión of tumor necrotic alpha and several antioxidant enzymes. Food Chem Toxicol. 2008, 46: 1111-1118. 10.1016/j.fct.2007.11.006.CrossRefPubMed
19.
Candy JM, Oakley AE, Klinowski J, Carpenter TA, Perry EK, Blessed G, Fairbairn A, Edwardson JA: Aluminosilicates and senile plaque formation in Alzheimer’s disease. Lancet. 1986, 1: 354-357.CrossRefPubMed
20.
Davenward S, Bentham P, Wright J, Crome P, Job D, Polwart A, Exley C: Silicon-rich mineral water as a non-invasive test of the ‘aluminum hypothesis’ in Alzheimer’s disease. J Alzheimers Dis. 2013, 33: 423-430.PubMed
21.
Miu AC: The silicon link between aluminium and Alzheimer’s disease. J Alzheimers Dis. 2006, 10: 39-42.PubMed
22.
Rondeau V, Jacqmin-Gadda H, Commenges D, Helmer C, Dartigues JF: Aluminum and Silica in drinking water and the risk of Alzheimer’s disease or cognitive cecline: findings from 15-year follow-up of the PAQUID cohort. Am J Epidemiol. 2009, 169: 489-496.CrossRefPubMed
23.
Kress M, Ried B, Reeh PW: Effects of oxygen radicals on nociceptive afferents in the rat skin in vitro. Pain. 1995, 62: 87-94. 10.1016/0304-3959(94)00254-C.CrossRefPubMed
24.
Waldron RT, Rozengurt E: Oxidative stress induces protein kinase D activation in intact cells. involvement of Src and dependence on protein kinase C. J Biol Chem. 2000, 275: 17114-17121. 10.1074/jbc.M908959199.CrossRefPubMed
25.
Bayani AA: Psychometric data for a Farsi translation of the Trait Meta-Mood Scale. Psychol Rep. 2009, 105: 198-204. 10.2466/PR0.105.1.198-204.CrossRefPubMed
26.
Bradford MA: Rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976, 72: 248-254. 10.1016/0003-2697(76)90527-3.CrossRefPubMed
27.
López E, Figueroa S, Oset-Gasque MJ, González MP: Apoptosis and necrosis: two distinct events induced by cadmium in cortical neurons in culture. Br J Pharmacol. 2003, 138: 901-911. 10.1038/sj.bjp.0705111.CrossRefPubMedPubMedCentral
28.
Mihara M, Uchiyama M: Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978, 86: 271-278. 10.1016/0003-2697(78)90342-1.CrossRefPubMed
29.
Nagai N, Ito Y: Excessive hydrogen peroxide enhances the attachment of amyloid β1-42 in the lens epithelium of UPL rats, a hereditary model for cataracts. Toxicology. 2014, 315: 55-64.CrossRefPubMed
30.
Xie H, Hou S, Jiang J, Sekutowicz M, Nelly J, Bacskai BJ: Rapid cell death is precede by amyloid plaque-mediated oxidative stress. Proc Natl Acad Sci U S A. 2013, 110: 7904-7909. 10.1073/pnas.1217938110.CrossRefPubMedPubMedCentral
31.
Chetsawang J, Govitrapong P, Chetsawang B: Hydrogen peroxide toxicity induces ras signaling in human neuroblastoma SH-SY5Y cultured cells. J Biomed Biotechnol. 2010, 2010: 803815-CrossRefPubMedPubMedCentral
32.
Steenland K, Ward E: Silica: a lung carcinogen. CA Cancer J Clin. 2014, 64: 63-69. 10.3322/caac.21214.CrossRefPubMed
33.
Zhou T, Rong Y, Liu Y, Zhou Y, Guo J, Cheng W, Wang H, Chen W: Association between proinflammatory responses of respirable silica dust and adverse health effects among dust-exposed workers. J Occup Environ Med. 2012, 54: 459-465. 10.1097/JOM.0b013e31824525ab.CrossRefPubMed
34.
Valencia A, Moran J: Reactive oxygen species induce different cell death mechanisms in cultured neurons. Free Radic Biol Med. 2004, 36: 1112-1125. 10.1016/j.freeradbiomed.2004.02.013.CrossRefPubMed
35.
Fatokun AA, Stone TW, Smith RA: Adenosine receptor ligands protect against a combination of apoptotic and necrotic cell death in cerebellar granule neurons. Exp Brain Res. 2008, 186: 151-160. 10.1007/s00221-007-1218-3.CrossRefPubMed
36.
Uttara B, Singh AV, Zamboni P, Mahajan RT: Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol. 2009, 7: 65-74. 10.2174/157015909787602823.CrossRefPubMedPubMedCentral
37.
Jenner P: Oxidative stress in Parkinson’s disease. Ann Neurol. 2003, 53: S26-S36. 10.1002/ana.10483.CrossRefPubMed
38.
Pratico D: Evidence of oxidative stress in Alzheimer’s disease brain and antioxidant therapy. Ann N Y Acad Sci. 2008, 1147: 70-78. 10.1196/annals.1427.010.CrossRefPubMed
39.
Allen CL, Bayraktutan U: Oxidative stress and its role in the pathogenesis of ischemic stroke. Int J Stroke. 2009, 4: 461-470. 10.1111/j.1747-4949.2009.00387.x.CrossRefPubMed
40.
Merino JJ, Oset-Gasque MJ: The CXCR7 activation by SDF1 induces neural progenitor migration (NPC): a dual effect on CXCR4/CXCR7 axis within the vascular niche of ischemic rats. Int J Stroke. 2013, 8: E56-10.1111/ijs.12174.CrossRefPubMed
41.
Ryter SW, Kim HP, Hoetzel A, Park JW, Nakahira K, Wang X, Choi AM: Mechanisms of cell death in oxidative stress. Antioxid Redox Signal. 2000, 9: 49-89.CrossRef
42.
Samuilov VD, Kiselevsky DB, Shestak AA, Nesov AV, Vasil’ev LA: Reactive oxygen species in programmed death of pea guard cells. Biochem-Moscow. 2008, 73: 1076-1084. 10.1134/S0006297908100039.CrossRef
43.
Sun B, Sun GB, Xiao J, Chen RC, Wang X, Wu Y, Cao L, Yang ZH, Sun XB: Isorhamnetin inhibits H2O2-induced activation of the intrinsic apoptotic pathway in H9c2 cardiomyocytes through scavenging reactive oxygen species and ERK inactivation. J Cell Biochem. 2012, 113: 473-485. 10.1002/jcb.23371.CrossRefPubMed
44.
Sui XQ, Xu ZM, Xie MB, Pei DA: Resveratrol inhibits hydrogen peroxide-induced apoptosis in endothelial cells via the activation of PI3K/Akt by miR-126. J Atheroscrerosc Thromb. 2014, 21: 108-118. 10.5551/jat.19257.CrossRef
45.
Okahashi N, Nakata M, Sumitomo T, Terao Y, Kawabata S: Hydrogen peroxide produced by oral Streptococci induces macrophage cell death. PLoS One. 2013, 8: 1-6.CrossRef
46.
Medan D, Wang L, Toledo D, Lu B, Stehlik C, Shi X, Rojanasakul Y: Regulation of Fas (CD95/ APO-1)-mediated apoptosis and necrosis by reactive oxygen species in macrophages. J Cell Physiol. 2005, 203: 78-84. 10.1002/jcp.20201.CrossRefPubMed
47.
Wang L, Azad N, Kongkaneramit L, Chen F, Lu Y, Jiang BH, Rojanasakul Y: The Fas death signalling pathway connecting reactive oxygen species generation and FLICE inhibitory protein down-regulation. J Immunol. 2008, 180: 3072-3080. 10.4049/jimmunol.180.5.3072.CrossRefPubMedPubMedCentral
Metadata
Title
Organic silicon protects human neuroblastoma SH-SY5Y cells against hydrogen peroxide effects
Authors
Alba Garcimartín
José J Merino
Maria Pilar González
Maria Isabel Sánchez-Reus
Francisco J Sánchez-Muniz
Sara Bastida
Juana Benedí
Publication date
01-12-2014
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2014
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/1472-6882-14-384

Other articles of this Issue 1/2014

BMC Complementary Medicine and Therapies 1/2014 Go to the issue

Research article

Standardizing therapeutic parameters of acupuncture for pain suppression in rats: preliminary study

Research article

Antihypertensive potential of the aqueous extract which combine leaf of Persea americana Mill. (Lauraceae), stems and leaf of Cymbopogon citratus (D.C) Stapf. (Poaceae), fruits of Citrus medical L. (Rutaceae) as well as honey in ethanol and sucrose experimental model

Research article

Chemical composition and biological activity of Rubus idaeus shoots – a traditional herbal remedy of Eastern Europe

Research article

Antioxidant activities of saponins extracted from Radix Trichosanthis: an in vivo and in vitro evaluation

Research article

Electroacupuncture improves cerebral blood flow and attenuates moderate ischemic injury via Angiotensin II its receptors-mediated mechanism in rats

Research article

Oral treatment with Euterpe oleracea Mart. (açaí) extract improves cardiac dysfunction and exercise intolerance in rats subjected to myocardial infarction