Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2012

Open Access 01-12-2012 | Research article

Glucan-rich polysaccharides from Pleurotus sajor-caju (Fr.) Singer prevents glucose intolerance, insulin resistance and inflammation in C57BL/6J mice fed a high-fat diet

Authors: Gowri Kanagasabapathy, Umah Rani Kuppusamy, Sri Nurestri Abd Malek, Mahmood Ameen Abdulla, Kek-Heng Chua, Vikineswary Sabaratnam

Published in: BMC Complementary Medicine and Therapies | Issue 1/2012

Login to get access

Abstract

Background

Pleurotus sajor-caju (P. sajor-caju) has been extremely useful in the prevention of diabetes mellitus due to its low fat and high soluble fiber content for thousands of years. Insulin resistance is a key component in the development of diabetes mellitus which is caused by inflammation. In this study, we aimed to investigate the in vivo efficacy of glucan-rich polysaccharide of P. sajor-caju (GE) against diabetes mellitus and inflammation in C57BL/6J mice fed a high-fat diet.

Methods

Diabetes was induced in C57BL/6J mice by feeding a high-fat diet. The mice were randomly assigned to 7 groups (n=6 per group). The control groups in this study were ND (for normal diet) and HFD (for high-fat diet). The treated groups were ND240 (for normal diet) (240 mg/kg b.w) and HFD60, HFD120 and HFD240 (for high-fat), where the mice were administrated with three dosages of GE (60, 120, 240 mg GE/kg b.w respectively). Metformin (2 mg/kg b.w) served as positive control. The glucose tolerance test, glucose and insulin levels were measured at the end of 16 weeks. Expressions of genes for inflammatory markers, GLUT-4 and adiponectin in the adipose tissue of the mice were assessed. One-way ANOVA and Duncan’s multiple range tests (DMRT) were used to determine the significant differences between groups.

Results

GE treated groups improved the glucose tolerance, attenuated hyperglycemia and hyperinsulinemia in the mice by up-regulating the adiponectin and GLUT-4 gene expressions. The mice in GE treated groups did not develop insulin resistance. GE also down-regulated the expression of inflammatory markers (IL-6, TNF-α, SAA2, CRP and MCP-1) via attenuation of nuclear transcription factors (NF-κB).

Conclusion

Glucan-rich polysaccharide of P. sajor-caju can serve as a potential agent for prevention of glucose intolerance, insulin resistance and inflammation.
Appendix
Available only for authorised users
Literature
1.
Simpson RW, Shaw JE, Zimmet PZ: The prevention of type 2 diabetes – lifestyle change or pharmacotherapy? A challenge for 21st century. Diabetes Res Clinical Prac. 2003, 59: 165-180. 10.1016/S0168-8227(02)00275-9.CrossRef
2.
Hamza N, Berke B, Cheze C, Agli AN, Robinson P, Gin H: Prevention of type 2 diabetes induced by high fat diet in the C57BL/6j mouse by two medicinal plants used in traditional treatment of diabetes in the east of Algeria. J Ethnopharmacol. 2010, 128: 513-518. 10.1016/j.jep.2010.01.004.CrossRefPubMed
3.
Umar AR, Ahmed QU, Muhammad BY, Dogarai BBS, Mat-Soad SZ: Anti-hyperglycemic activity of the leaves of Tetracera scandens Linn. Merr. in allaxon induced diabetic rats. J Ethnopharmacol. 2010, 131: 140-145. 10.1016/j.jep.2010.06.016.CrossRefPubMed
4.
Yumuk VD, Hatemi H, Tarakchi T, Uyar N, Turan N, Bagriacik N: High prevalence of obesity and diabetes mellitus in Konya, a central Anatolian city in Turkey. Diabetes Res Clinical Prac. 2005, 70: 151-158. 10.1016/j.diabres.2005.03.030.CrossRef
5.
Bailey CJ: Insulin resistance and antidiabetic drugs. Biochem Pharmacol. 1999, 58: 1511-1520. 10.1016/S0006-2952(99)00191-4.CrossRefPubMed
6.
Slovacek L, Pavlik V, Slovackova B: The effect of sibutramine therapy on occurance of depression symptoms among obese patients. Nutr Metab Cardiovascular Disease. 2008, 18: 43-44.CrossRef
7.
Jie J, Zhang X, Hu YS, Wu Y, Wang QZ, Li NN: Evaluation of in vivo antioxidant activities of Ganoderma lucidum polysaccharides in STZ-diabetic rats. Food Chem. 2009, 115: 32-36. 10.1016/j.foodchem.2008.11.043.CrossRef
8.
Wasser SP: Current findings, future trends, and unsolved problems in studies of medicinal mushrooms. App Microbiol Biotechnol. 2011, 89: 1323-1332. 10.1007/s00253-010-3067-4.CrossRef
9.
Cimerman NG: Medicinal value of the genus Pleurotus (Fr.) P.Karst. (Agaricales s.i., Basidiomycetes). Int J Med Mushrooms. 1999, 1: 69-80.CrossRef
10.
Pramanik M, Mondal S, Chakraborthy I, Rout D, Islam SS: Structural analysis of a water-soluable glucan (Fr I) of an edible mushroom, Pleurotus sajor-caju. Carbohydrate Res. 2007, 342: 2670-2675. 10.1016/j.carres.2007.08.012.CrossRef
11.
Schneider I, Kressel G, Meyer A, Krings U, Berger RG, Hahn A: Lipid lowering effects of oyster mushroom (Pleurotus ostreatus) in humans. J Functional Foods. 2011, 3: 17-24. 10.1016/j.jff.2010.11.004.CrossRef
12.
Moon HS, Lee HG, Seo GH, Chung CH, Kim TG, Choi YJ: Antiobesity effect of PEGylated conjugated linoleic acid on high fat diet induced obese mice C57BL/6J (ob/ob) mice: attenuation of insulin resistance and enhancement of antioxidant defenses. J Nutr Biochem. 2009, 20: 187-194. 10.1016/j.jnutbio.2008.02.001.CrossRefPubMed
13.
Roy SK, Maiti D, Mondal S, Das D, Islam SS: Structural analysis of a polysaccharide isolated from the aqueous extract of an edible mushroom, Pleurotus sajor-caju, cultivar Black Japan. Carbohydrate Res. 2008, 343: 1108-1113. 10.1016/j.carres.2008.02.015.CrossRef
14.
Lee HS, Park HJ, Chun HK, Cho SY, Cho SM, Lillehoj HS: Dietary phytic acids lowers the blood glucose level in diabetic KK mice. Nutr Res. 2006, 26: 474-479. 10.1016/j.nutres.2006.06.017.CrossRef
15.
Mathews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turmer RC: Homeostasis model assessment: insulin resistant and B-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia. 1985, 28: 412-419. 10.1007/BF00280883.CrossRef
16.
Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2 –ΔΔCt method. Methods. 2001, 25: 402-408. 10.1006/meth.2001.1262.CrossRefPubMed
17.
Pramanik M, Mondal S, Chakraborthy I, Rout D, Islam SS: Structural investigation of polysaccharides (Fr II) isolated from aqueous extract of an edible mushroom, Pleurotus sajor-caju. Carbohydr Res. 2005, 340: 629-636. 10.1016/j.carres.2004.12.032.CrossRefPubMed
18.
Scheja L, Heese B, Seedorf K: Beneficial effects of IKK –deficiency on body weight and insulin sensitivity are lost in high fat diet-induced obesity in mice. Biochem Biophysical Res Comm. 2011, 407: 288-294. 10.1016/j.bbrc.2011.02.137.CrossRef
19.
Roberts CK, Sindhu KK: Oxidative stress and metabolic syndrome. Life Sci. 2009, 84: 705-712. 10.1016/j.lfs.2009.02.026.CrossRefPubMed
20.
Nagata M, Ijichi S, Hashiguchi T, Morioka H, Arimura K: Metabolic syndrome-related markers in obese university students in Kagoshima, Japan: Implications of uric acid and plasminogen activator inhibitor type-1. Diabetes Metab Syndrome: Clinical Res Rev. 2009, 3: 96-102. 10.1016/j.dsx.2009.02.009.CrossRef
21.
Ando K, Fujita T: Metabolic syndrome and oxidative stress. Free Radical Biol Med. 2009, 47: 213-218. 10.1016/j.freeradbiomed.2009.04.030.CrossRef
22.
Zou C, Shao J: Role of adipocytokines in obesity-associated insulin resistance. J Nutr Biochem. 2008, 19: 277-286. 10.1016/j.jnutbio.2007.06.006.CrossRefPubMed
23.
Shoelson SE, Laura H, Naaz A: Obesity, inflammation and insulin resistance. Gastroenterol. 2007, 132: 2169-2180. 10.1053/j.gastro.2007.03.059.CrossRef
24.
Bonnard C, Durand A, Vidal H, Rieusset J: Changes in adiponectin, its receptor and AMPK activity in tissues of diet induced-diabetic mice. Diabetes Metab. 2008, 34: 52-61. 10.1016/j.diabet.2007.09.006.CrossRefPubMed
25.
Lee L, Alloosh M, Saxena R, Van-Alstine W, Watkins BA, Klaunig JE: Nutritional model of steatohepatitis and metabolic syndrome in the ossabaw miniature swine. Hepatol. 2009, 50: 56-67. 10.1002/hep.22904.CrossRef
26.
Peng Y, Rideout D, Rakita S, Sajan M, Farese R, You M: Downregulation of adiponectin/adipor2 is associated with steatohepatitis in obese mice. J Gastrointestinal Surgery. 2009, 13: 2043-2049. 10.1007/s11605-009-1032-2.CrossRef
27.
Yang RZ, Lee MJ, Hu H, Pollin TI, Ryan AS, Nicklas BJ: Acute-phase serum amyloid A: an inflammatory adipokine and potential link between obesity and its metabolic complications. PLoS Med. 2006, 3: e287-10.1371/journal.pmed.0030287.CrossRefPubMedPubMedCentral
28.
Townsend KL, Lorenzi MM, Widmaier EP: High-fat diet-induced changes in body mass and hypothalamic gene expression in wild-type and leptin-deficient mice. Endocrine. 2008, 33: 176-188. 10.1007/s12020-008-9070-1.CrossRefPubMed
29.
Zhang W, Liu CQ, Wang PW, Sun SY, Su WJ, Zhang HJ: Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet. Eur J Pharmacol. 2010, 649: 398-402. 10.1016/j.ejphar.2010.09.054.CrossRefPubMed
30.
Miura S, Tsunoda N, Ikeda S, Kai Y, Ono M, Maruyama K: Regulatory sequence elements of mouse GLUT4 gene expression in adipose tissue. Biochem Biophys Res Comm. 2003, 312: 277-284. 10.1016/j.bbrc.2003.10.114.CrossRefPubMed
31.
Graham TE, Yang Q, Bluher M, Hammarstedt A, Ciaraldi TP, Henry RR: Retinol-binding protein 4 and insulin resistance in lean, obese and diabetic subjects. Eng J Med. 2006, 354: 2552-2563. 10.1056/NEJMoa054862.CrossRef
32.
Wang C, Mao X, Wang L, Liu M, Wetzel MD, Guan KL: Adiponectin sensitizes insulin signaling by reducing p70 S6 kinase-mediated serine phosphorylation of IRS-1. J Biol Chem. 2007, 282: 1991-1996.
33.
Balagopal P, Graham TE, Kahn BB, Altomare A, Funanage V, George D: Reduction of elevated serum retinol-binding protein 4 in obese children lifestyle intervention: association with sub-clinical inflammation. J Clinical Endocrinol Metab. 2007, 7: 2006-2012.
34.
Ferrante AW: Obesity induced inflammation: a metabolic dialogue in the language of inflammation. J Int Med. 2007, 262: 408-414. 10.1111/j.1365-2796.2007.01852.x.CrossRef
35.
Dandona P, Aljada A, Bandyopadhyay A: Inflammation: the link between insulin resistance, obesity and diabetes. Trends Immunol. 2004, 25: 4-7. 10.1016/j.it.2003.10.013.CrossRefPubMed
36.
Mohanty P, Aljada A, Ghanim H, Hofmeyer D, Tripathy D, Syed T: Evidence for a potent anti-inflammatory effect of rosiglitazone. J Clinical Endocrinol Metab. 2004, 89: 2728-2735. 10.1210/jc.2003-032103.CrossRef
37.
Kuhad A, Chopra K: Attenuation of diabetic nephropathy by tocotrienol; involvement of NFKB signaling pathway. Life Sci. 2009, 84: 296-301. 10.1016/j.lfs.2008.12.014.CrossRefPubMed
38.
Terra X, Pallares V, Ardevol A, Blade C, Larrea JF, Pujadas G: Modulatory effect of grape seed procyanidins on local systemic inflammation in diet induced obesity rats. J Nutr Biochem. 2011, 22: 380-387. 10.1016/j.jnutbio.2010.03.006.CrossRefPubMed
39.
Kern PA, Di G, Gregoria B, Lu T, Rassouli N, Ranganathan G: Adiponectin expression from human adipose tissue: relation to obesity, insulin resistance and TNF-α expression. Diabetes. 2001, 52: 1779-1785.CrossRef
40.
Kim SO, Yun SJ, Jung B, Lee EH, Hahm DH, Shim I: Hypolipidemic effects of crude extract of adlay seed in obesity rat fed high fat diet: relations of TNF-α and leptin mRNA expressions and serum lipid levels. Life Sci. 2004, 75: 1391-1404. 10.1016/j.lfs.2004.03.006.CrossRefPubMed
41.
Koh KK, Han SH, Quon MJ: Inflammatory markers and the metabolic syndrome; insight from therapeutic interventions. J Am Coll Cardiol. 2005, 46: 1978-1985. 10.1016/j.jacc.2005.06.082.CrossRefPubMed
42.
Pannacciulli N, Cantatore FP, Minenna A, Bellacicco M, Giorgino R, De-Pergola G: C-reactive protein is independently associated with total body fat, central fat, and insulin resistance in adult women. Int J Obesity Related Metab Disorder. 2001, 25: 1416-1420. 10.1038/sj.ijo.0801719.CrossRef
Metadata
Title
Glucan-rich polysaccharides from Pleurotus sajor-caju (Fr.) Singer prevents glucose intolerance, insulin resistance and inflammation in C57BL/6J mice fed a high-fat diet
Authors
Gowri Kanagasabapathy
Umah Rani Kuppusamy
Sri Nurestri Abd Malek
Mahmood Ameen Abdulla
Kek-Heng Chua
Vikineswary Sabaratnam
Publication date
01-12-2012
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2012
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/1472-6882-12-261

Other articles of this Issue 1/2012

BMC Complementary Medicine and Therapies 1/2012 Go to the issue

Research article

Inhibition of lipopolysaccharide-induced nitric oxide and prostaglandin E2 production by chloroform fraction of Cudrania tricuspidata in RAW 264.7 macrophages

Debate

Acupuncture as a therapeutic treatment option for threatened miscarriage

Research article

Assessment of anti-depressant effect of nelumbinis semen on rats under chronic mild stress and its subchronic oral toxicity in rats and beagle dogs

Research article

Effect of aqueous extract of Arctium lappa L. (burdock) roots on the sexual behavior of male rats

Research article

Prakriti (Ayurvedic concept of constitution) and variations in platelet aggregation

Research article

Polar extracts from (Tunisian) Acacia salicina Lindl. Study of the antimicrobial and antigenotoxic activities