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BMC Complementary Medicine and Therapies

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Open Access 01-12-2012 | Research article

Anti-proliferative effect of methanolic extract of Gracilaria tenuistipitata on oral cancer cells involves apoptosis, DNA damage, and oxidative stress

Authors: Chi-Chen Yeh, Jing-Iong Yang, Jin-Ching Lee, Chao-Neng Tseng, Ya-Ching Chan, You-Cheng Hseu, Jen-Yang Tang, Li-Yeh Chuang, Hurng-Wern Huang, Fang-Rong Chang, Hsueh-Wei Chang

Published in: BMC Complementary Medicine and Therapies | Issue 1/2012

Abstract

Background

Methanolic extracts of Gracilaria tenuistipitata (MEGT) were obtained from the edible red algae. Previously, we found that water extract of G. tenuistipitata was able to modulate oxidative stress-induced DNA damage and its related cellular responses.

Methods

In this study, the methanol extraction product MEGT was used to evaluate the cell growth inhibition in oral cancer cells and its possible mechanism was investigated.

Results

The cell viability of MEGT treated Ca9-22 oral cancer cell line was significantly decreased in a dose–response manner (p < 0.05). The sub-G1 population and annexin V intensity of MEGT-treated Ca9-22 cancer cells were significantly increased in a dose–response manner (p < 0.0005 and p < 0.001, respectively). The γH2AX intensities of MEGT-treated Ca9-22 cancer cells were significantly increased in a dose–response manner (p < 0.05). The reactive oxygen species (ROS) and glutathione (GSH)-positive intensities of MEGT-treated Ca9-22 oral cancer cells were significantly increased and decreased, respectively, in a dose–response manner (p < 0.05). The DiOC₂(3) intensity for mitochondrial membrane potential (MMP) of MEGT-treated Ca9-22 cancer cells was significantly decreased in a dose–response manner (p < 0.05).

Conclusions

These results indicated that MEGT had apoptosis-based cytotoxicity against oral cancer cells through the DNA damage, ROS induction, and mitochondrial depolarization. Therefore, MEGT derived from the edible algae may have potential therapeutic effects against oral squamous cell carcinoma (OSCC).

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/12/142/prepub

Title: Anti-proliferative effect of methanolic extract of Gracilaria tenuistipitata on oral cancer cells involves apoptosis, DNA damage, and oxidative stress
Authors: Chi-Chen Yeh
Jing-Iong Yang
Jin-Ching Lee
Chao-Neng Tseng
Ya-Ching Chan
You-Cheng Hseu
Jen-Yang Tang
Li-Yeh Chuang
Hurng-Wern Huang
Fang-Rong Chang
Hsueh-Wei Chang
Publication date: 01-12-2012
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2012
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/1472-6882-12-142

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Anti-proliferative effect of methanolic extract of Gracilaria tenuistipitata on oral cancer cells involves apoptosis, DNA damage, and oxidative stress

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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