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BMC Physiology
Published in: BMC Physiology 1/2005

Open Access 01-12-2005 | Research article

Troglitazone, a PPAR-γ activator prevents endothelial cell adhesion molecule expression and lymphocyte adhesion mediated by TNF-α

Authors: Makoto Sasaki, Paul Jordan, Tomas Welbourne, Alireza Minagar, Takashi Joh, Makoto Itoh, John W Elrod, J Steven Alexander

Published in: BMC Physiology | Issue 1/2005

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Abstract

Background

Cytokine mediated induction of the mucosal addressin cell adhesion molecule-1(MAdCAM-1) expression is associated with the onset and progression of inflammatory bowel disease (IBD).

Results

Using western blotting and cell-based ELISA, we show in this study that troglitazone, an activator of the peroxisome proliferator-activated receptor-γ (PPAR-γ), widely used in the treatment of diabetes, has as well recently been highlighted as protective in models of inflammation and cancer. We found that troglitazone (10–40 μM), significantly reduced the TNF-α (1 ng/ml) mediated induction of endothelial MAdCAM-1 in a dose-dependent manner, achieving a 34.7% to 98.4% reduction in induced MAdCAM-1. Trogliazone (20μM) reduced TNF-α induced VCAM-1, ICAM-1 and E-selectin expression. Moreover, troglitazone significantly reduced α4β7-integrin dependent lymphocyte adhesion to TNF-α cultured endothelial cells.

Conclusions

These results suggest that PPAR-γ agonists like troglitazone may be useful in the clinical treatment of IBD.
Appendix
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Metadata
Title
Troglitazone, a PPAR-γ activator prevents endothelial cell adhesion molecule expression and lymphocyte adhesion mediated by TNF-α
Authors
Makoto Sasaki
Paul Jordan
Tomas Welbourne
Alireza Minagar
Takashi Joh
Makoto Itoh
John W Elrod
J Steven Alexander
Publication date
01-12-2005
Publisher
BioMed Central
Published in
BMC Physiology / Issue 1/2005
Electronic ISSN: 1472-6793
DOI
https://doi.org/10.1186/1472-6793-5-3

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