Top

Published in:

Open Access 01-12-2008 | Research article

Extracorporeal shock waves down-regulate the expression of interleukin-10 and tumor necrosis factor-alpha in osteoarthritic chondrocytes

Authors: Biagio Moretti, Florenzo Iannone, Angela Notarnicola, Giovanni Lapadula, Lorenzo Moretti, Vittorio Patella, Raffaele Garofalo

Published in: BMC Musculoskeletal Disorders | Issue 1/2008

Abstract

Background

The purpose of this study was to investigate the effects of extra corporeal shock waves (ESW) therapy on the metabolism of healthy and osteoarthritic human chondrocytes, and particularly on the expression of IL-10, TNF-alpha and beta1 integrin.

Methods

Human adult articular cartilage was obtained from 9 patients (6 male and 3 females), with primary knee osteoarthritis (OA), undergoing total joint replacement and from 3 young healthy donors (HD) (2 males, 1 female) with joint traumatic fracture. After isolation, chondrocytes underwent ESW treatment (electromagnetic generator system, MINILITH SL1, STORZ MEDICAL) at different parameters of impulses, energy levels and energy flux density. After that, chondrocytes were cultured in 24-well plate in DMEM supplemented with 10% FCS for 48 hours and then beta₁ integrin surface expression and intracellular IL-10 and TNF-alpha levels were evaluated by flow-cytometry.

Results

At baseline, osteoarthritic chondrocytes expressed significantly lower levels of beta1 integrin and higher levels and IL-10 and TNF-alpha levels. Following ESW application, while beta1 integrin expression remain unchanged, a significant decrease of IL-10 and TNF-alpha intracellular levels was observed both in osteoarthritic and healthy chondrocytes. IL-10 levels decreased at any impulses and energy levels, while a significant reduction of TNF-alpha was mainly found at middle energies.

Conclusion

Our study confirmed that osteoarthritic chondrocytes express low beta₁ integrin and high TNF-alpha and IL-10 levels. Nonetheless, ESW treatment application down-regulate the intracellular levels of TNF-alpha and IL-10 by chondrocytes, suggesting that ESW might restore TNF-alpha and IL-10 production by osteoarthritic chondrocytes at normal levels. However, further in vivo and in vitro studies are necessary to establish if ESW can represent a viable option in the treatment of OA.

Available only for authorised users

Dahlberg J, Fitch G, Evans RB, McClure SR, Conzemius M: The evaluation of extracorporeal shockwave therapy in naturally occurring osteoarthritis of the stifle joint in dogs. Vet Comp Orthop Traumatol. 2005, 18 (3): 147-152.PubMed

Revenaugh MS: Extracorporeal shock wave therapy for treatment of osteoarthritis in the horse: clinical applications. Vet Clin North Am Equine Pract. 2005, 21 (3): 609-25. 10.1016/j.cveq.2005.09.001.CrossRefPubMed

Iannone F, Lapadula G: The pathophysiology of osteoarthritis. Aging Clin Exp Res. 2003, 15 (5): 364-372.CrossRefPubMed

Damsky CH, Ilic D: Integrin signaling: it's where the action is. Curr Opin Cell Biol. 2002, 14 (5): 594-602. 10.1016/S0955-0674(02)00368-X.CrossRefPubMed

Lapadula G, Iannone F, Zuccaro C, Grattagliano V, Covelli M, Patella V, Lo Bianco G, Pipitone V: Chondrocyte phenotyping in human osteoarthritis. Clin Rheumatol. 1998, 17 (2): 99-104. 10.1007/BF01452253.CrossRefPubMed

Westacott CI, Atkins RM, Dieppe PA, Elson CJ: Tumor necrosis factor-alpha receptor expression on chondrocytes isolated from human articular cartilage. J Rheumatol. 1994, 21 (9): 1710-1715.PubMed

Alaaeddine N, DiBattista JA, Pelletier JP, Cloutier JM, Kiansa K, Dupuis M: Osteoarthritic synovial fibroblasts possess an increased level of tumor necrosis factor-receptor 55 (TNF-R55) that mediates biological activation by TNF-alpha. J Rheumatol. 1997, 24 (10): 1985-1994.PubMed

de Waal MR, Abrams J, Bennett B, Figdor CG, de Vries JE: Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes. J Exp Med. 1991, 174 (5): 1209-1220. 10.1084/jem.174.5.1209.CrossRef

Rousset F, Garcia E, Defrance T, Peronne C, Vezzio N, Hsu DH, Kastelein R, Moore Kw, Banchereau J: Interleukin 10 is a potent growth and differentiation factor for activated human B lymphocytes. Proc Natl Acad Sci USA. 1992, 89 (5): 1890-1893. 10.1073/pnas.89.5.1890.CrossRefPubMedPubMedCentral

10.

Iannone F, De Bari C, Dell'Accio F, Covelli M, Cantatore FP, Patella V, Lo Bianco G, Lapadula G: Interleukin-10 and interleukin-10 receptor in human osteoarthritic and healthy chondrocytes. Clin Exp Rheumatol. 2001, 19 (2): 139-145.PubMed

11.

Westacott CI, Barakat AF, Wood L, Perry MJ, Neison P, Bisbinas I, Armstrong L, Millar Ab, Elson Cj: Tumor necrosis factor alpha can contribute to focal loss of cartilage in osteoarthritis. Osteoarthritis Cartilage. 2000, 8 (3): 213-221. 10.1053/joca.1999.0292.CrossRefPubMed

12.

Lapadula G, Iannone F, Zuccaro C, Grattagliano V, Covelli M, Patella V, Lo Bianco G, Pipitone V: Integrin expression on chondrocytes: correlations with the degree of cartilage damage in human osteoarthritis. Clin Exp Rheumatol. 1997, 15 (3): 247-254.PubMed

13.

Webb GR, Westacott CI, Elson CJ: Chondrocyte tumor necrosis factor receptors and focal loss of cartilage in osteoarthritis. Osteoarthritis Cartilage. 1997, 5 (6): 427-437. 10.1016/S1063-4584(97)80047-7.CrossRefPubMed

14.

Hui W, Barksby HE, Young DA, Cawston TE, McKie N, Rowan AD: Oncostatin M in combination with tumour necrosis factor {alpha} induces a chondrocyte membrane associated aggrecanase that is distinct from ADAMTS aggrecanase-1 or -2. Ann Rheum Dis. 2005, 64 (11): 1624-1632. 10.1136/ard.2004.028191.CrossRefPubMedPubMedCentral

15.

Liacini A, Sylvester J, Li WQ, Huang W, Dehnade F, Ahmad M, Zafarullah M: Induction of matrix metalloproteinase-13 gene expression by TNF-alpha is mediated by MAP kinases, AP-1, and NF-kappaB transcription factors in articular chondrocytes. Exp Cell Res. 2003, 288 (1): 208-217. 10.1016/S0014-4827(03)00180-0.CrossRefPubMed

16.

Van Bezooijen RL, Wee-Pals L, Papapoulos SE, Lowik CW: Interleukin 17 synergises with tumour necrosis factor alpha to induce cartilage destruction in vitro. Ann Rheum Dis. 2002, 61 (10): 870-876. 10.1136/ard.61.10.870.CrossRefPubMedPubMedCentral

17.

Joosten LA, Lubberts E, Durez P, Helsen MM, Jacobs MJ, Goldman M, van den Berg WB: Role of interleukin-4 and interleukin-10 in murine collagen-induced arthritis. Protective effect of interleukin-4 and interleukin-10 treatment on cartilage destruction. Arthritis Rheum. 1997, 40 (2): 249-260. 10.1002/art.1780400209.CrossRefPubMed

18.

Reitamo S, Remitz A, Tamai K, Uitto J: Interleukin-10 modulates type I collagen and matrix metalloprotease gene expression in cultured human skin fibroblasts. J Clin Invest. 1994, 94 (6): 2489-2492. 10.1172/JCI117618.CrossRefPubMedPubMedCentral

19.

Dorotka R, Kubista B, Schatz KD, Trieb K: Effects of extracorporeal shock waves on human articular chondrocytes and ovine bone marrow stromal cells in vitro. Arch Orthop Trauma Surg. 2003, 123 (7): 345-348. 10.1007/s00402-003-0551-7.CrossRefPubMed

20.

Katsikis PD, Chu CQ, Brennan FM, Maini RN, Feldmann M: Immunoregulatory role of interleukin 10 in rheumatoid arthritis. J Exp Med. 1994, 179 (5): 1517-1527. 10.1084/jem.179.5.1517.CrossRefPubMed

The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2474/9/16/prepub

Title: Extracorporeal shock waves down-regulate the expression of interleukin-10 and tumor necrosis factor-alpha in osteoarthritic chondrocytes
Authors: Biagio Moretti
Florenzo Iannone
Angela Notarnicola
Giovanni Lapadula
Lorenzo Moretti
Vittorio Patella
Raffaele Garofalo
Publication date: 01-12-2008
Publisher: BioMed Central
Published in: BMC Musculoskeletal Disorders / Issue 1/2008
Electronic ISSN: 1471-2474
DOI: https://doi.org/10.1186/1471-2474-9-16

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Extracorporeal shock waves down-regulate the expression of interleukin-10 and tumor necrosis factor-alpha in osteoarthritic chondrocytes

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 1/2008

Outcome analysis following removal of locking plate fixation of the proximal humerus

Clinical knee findings in floor layers with focus on meniscal status

Long-term retention on treatment with lumiracoxib 100 mg once or twice daily compared with celecoxib 200 mg once daily: A randomised controlled trial in patients with osteoarthritis

The orthopaedic trauma literature: an evaluation of statistically significant findings in orthopaedic trauma randomized trials

The reliability of knee joint position testing using electrogoniometry

Foot orthoses and physiotherapy in the treatment of patellofemoral pain syndrome: A randomised clinical trial