Top

Published in:

Open Access 01-12-2009 | Research article

Elevation of Bombina variegata peptide 8 in mice with collagen-induced arthritis

Authors: Daitaro Kurosaka, Kentaro Noda, Ken Yoshida, Kazuhiro Furuya, Taro Ukichi, Eigo Takahashi, Maimi Yanagimachi, Isamu Kingetsu, Saburo Saito, Akio Yamada

Published in: BMC Musculoskeletal Disorders | Issue 1/2009

Abstract

Background

Bombina variegate peptide 8 (Bv8) is a small protein secreted by frog skin. Recently it has been shown to contribute to tumor angiogenesis in mouse model. The purpose of this study was to investigate Bv8 in mice with type II collagen-induced arthritis (CIA).

Methods

We induced CIA in male DBA/1J mice. The severity of arthritis was evaluated based on an arthritis score. RNA was extracted from the joint, and examined for Bv8 mRNA expression by RT-PCR and real-time RT-PCR. Synovial tissue and bone marrow were immunohistochemically examined using anti-Bv8 antibody.

Results

The level of Bv8 mRNA expression in the joint was below the detection limit in the control group, but was elevated in the CIA group, and was correlated with the arthritis score. In addition, an increase in Bv8-positive cells was observed in the synovium and bone marrow in the CIA group.

Conclusion

Bv8 was elevated in the synovium and bone marrow of CIA mice, suggesting that Bv8 plays an important role in the pathogenesis of arthritis.

Available only for authorised users

Mollay C, Wechselberger C, Mignogna G, Negri L, Melchiorri P, Barra D, Kreil G: Bv8, a small protein from frog skin and its homologue from snake venom induce hyperalgesia in rats. Eur J Pharmacol. 1999, 374: 189-196. 10.1016/S0014-2999(99)00229-0.CrossRefPubMed

Wechselberger C, Puglisi R, Engel E, Lepperdinger G, Boitani C, Kreil G: The mammalian homologues of frog Bv8 are mainly expressed in spermatocytes. FEBS Lett. 1999, 462: 177-181. 10.1016/S0014-5793(99)01473-8.CrossRefPubMed

Ferrara N, LeCouter J, Lin R, Peale F: EG-VEGF and Bv8: a novel family of tissue-restricted angiogenic factors. Biochim Biophys Acta. 2004, 1654: 69-78.PubMed

LeCouter J, Lin R, Tejada M, Frantz G, Peale F, Hillan KJ, Ferrara N: The endocrine-gland-derived VEGF homologue Bv8 promotes angiogenesis in the testis: Localization of Bv8 receptors to endothelial cells. Proc Natl Acad Sci USA. 2003, 100: 2685-2690. 10.1073/pnas.0337667100.CrossRefPubMedPubMedCentral

Negri L, Lattanzi R, Giannini E, Melchiorri P: Bv8/Prokineticin proteins and their receptors. Life Sci. 2007, 81: 1103-1116. 10.1016/j.lfs.2007.08.011.CrossRefPubMed

Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N: Bv8 regulates myeloid-cell-dependent tumour angiogenesis. Nature. 2007, 450: 825-831. 10.1038/nature06348.CrossRefPubMed

Kurosaka D, Yasuda J, Yoshida K, Yasuda C, Toyokawa Y, Yokoyama T, Kingetsu I, Yamada A: Kinetics of circulating endothelial progenitor cells in mice with type II collagen arthritis. Blood Cells Mol Dis. 2005, 35: 236-240. 10.1016/j.bcmd.2005.06.001.CrossRefPubMed

Kurosaka D, Yoshida K, Yasuda J, Yasuda C, Noda K, Furuya K, Ukichi T, Kingetsu I, Joh K, Yamaguchi N, Saito S, Yamada A: The effect of endostatin evaluated in an experimental animal model of collagen-induced arthritis. Scand J Rheumatol. 2007, 36: 434-441. 10.1080/03009740701605913.CrossRefPubMed

Wong PK, Egan PJ, Croker BA, O'Donnell K, Sims NA, Drake S, Kiu H, McManus EJ, Alexander WS, Roberts AW, Wicks IP: SOCS-3 negatively regulates innate and adaptive immune mechanisms in acute IL-1-dependent inflammatory arthritis. J Clin Invest. 2006, 116: 1571-1581. 10.1172/JCI25660.CrossRefPubMedPubMedCentral

10.

Yim YK, Lee H, Hong KE, Kim YI, Lee BR, Son CG, Kim JE: Electro-acupuncture at acupoint ST36 reduces inflammation and regulates immune activity in Collagen-Induced Arthritic Mice. Evid Based Complement Alternat Med. 2007, 4: 51-57. 10.1093/ecam/nel054.CrossRefPubMed

11.

Cheng MY, Bullock CM, Li C, Lee AG, Bermak JC, Belluzzi J, Weaver DR, Leslie FM, Zhou QY: Prokineticin 2 transmits the behavioural circadian rhythm of the suprachiasmatic nucleus. Nature. 2002, 417: 405-410. 10.1038/417405a.CrossRefPubMed

12.

Cutolo M, Straub RH: Circadian rhythms in arthritis: hormonal effects on the immune/inflammatory reaction. Autoimmun Rev. 2008, 7: 223-228. 10.1016/j.autrev.2007.11.019.CrossRefPubMed

13.

Seres J, Herichová I, Roman O, Bornstein S, Jurcovicová J: Evidence for daily rhythms of the expression of proopiomelanocortin, interleukin-1-beta and interleukin-6 in adenopituitaries of male long-evans rats: effect of adjuvant arthritis. Neuroimmunomodulation. 2004, 11: 316-322. 10.1159/000079412.CrossRefPubMed

14.

Esquifino AI, Cano P, Jiménez-Ortega V, Fernández-Mateos P, Cardinali DP: Neuroendocrine-immune correlates of circadian physiology: studies in experimental models of arthritis, ethanol feeding, aging, social isolation, and calorie restriction. Endocrine. 2007, 32: 1-19. 10.1007/s12020-007-9009-y.CrossRefPubMed

15.

Shojaei F, Wu X, Malik AK, Zhong C, Baldwin ME, Schanz S, Fuh G, Gerber HP, Ferrara N: Tumor refractoriness to anti-VEGF treatment is mediated by CD11b+Gr1+ myeloid cells. Nat Biotechnol. 2007, 25: 911-920. 10.1038/nbt1323.CrossRefPubMed

16.

Shojaei F, Ferrara N: Refractoriness to antivascular endothelial growth factor treatment: role of myeloid cells. Cancer Res. 2008, 68: 5501-5504. 10.1158/0008-5472.CAN-08-0925.CrossRefPubMed

17.

Lawlor KE, Campbell IK, Metcalf D, O'Donnell K, van Nieuwenhuijze A, Roberts AW, Wicks IP: Critical role for granulocyte colony-stimulating factor in inflammatory arthritis. Proc Natl Acad Sci USA. 2004, 101: 11398-11403. 10.1073/pnas.0404328101.CrossRefPubMedPubMedCentral

The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2474/10/45/prepub

Title: Elevation of Bombina variegata peptide 8 in mice with collagen-induced arthritis
Authors: Daitaro Kurosaka
Kentaro Noda
Ken Yoshida
Kazuhiro Furuya
Taro Ukichi
Eigo Takahashi
Maimi Yanagimachi
Isamu Kingetsu
Saburo Saito
Akio Yamada
Publication date: 01-12-2009
Publisher: BioMed Central
Published in: BMC Musculoskeletal Disorders / Issue 1/2009
Electronic ISSN: 1471-2474
DOI: https://doi.org/10.1186/1471-2474-10-45

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Elevation of Bombina variegata peptide 8 in mice with collagen-induced arthritis

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 1/2009

Inter-rater reliability of three standardized functional tests in patients with low back pain

The effects of exercise and weight loss in overweight patients with hip osteoarthritis: design of a prospective cohort study

Postural changes in women with chronic pelvic pain: a case control study

Empirical evaluation of the inter-relationship of articular elements involved in the pathoanatomy of knee osteoarthritis using Magnetic Resonance Imaging

A walking programme and a supervised exercise class versus usual physiotherapy for chronic low back pain: a single-blinded randomised controlled trial. (The Supervised Walking In comparison to Fitness Training for Back Pain (SWIFT) Trial)

Increased hallux angle in children and its association with insufficient length of footwear: A community based cross-sectional study