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Open Access 01-12-2007 | Research article

Common NOD2/CARD15 variants are not associated with susceptibility or the clinicopathologic characteristics of sporadic colorectal cancer in Hungarian patients

Authors: Peter Laszlo Lakatos, Erika Hitre, Ferenc Szalay, Kerstin Zinober, Peter Fuszek, Laszlo Lakatos, Simon Fischer, Janos Osztovits, Orsolya Gemela, Gabor Veres, Janos Papp, Peter Ferenci

Published in: BMC Cancer | Issue 1/2007

Abstract

Background

Epidemiological observations suggest that cancer arises from chronically inflamed tissues. Inflammatory bowel disease (IBD) is a typical example as patients with longstanding IBD are at an increased risk for developing colorectal cancer (CRC) and mutations of the NOD2/CARD15 gene increase the risk for Crohn's disease (CD). Recently, NOD2/CARD15 has been associated with a risk for CRC in some studies, which stemmed from ethnically diverse populations. Our aim was to identify common NOD2/CARD15 mutations in Hungarian patients with sporadic CRC.

Methods

A total of 194 sporadic CRC patients (m/f: 108/86, age at diagnosis of CRC: 63.2 ± 9.1 years old) and 200 healthy subjects were included. DNA was screened for SNP8, SNP12 and SNP13 NOD2/CARD15 mutations by denaturing-HPLC and confirmed by direct sequencing.

Results

NOD2/CARD15 mutations were found in 28 patients (14.4%) and in 23 controls (11.5%, p = NS). Allele frequencies for SNP8/R702W (1.8% vs. 1.5%) SNP12/G908R (1.8% vs. 1.8%) and SNP13/3020insC (3.6% vs. 2.5%) were also not statistically different between patients and controls. The clinicopathologic characteristics of CRC patients with or without NOD2/CARD15 mutations were not significantly different.

Conclusion

Our results suggest that common NOD2/CARD15 mutations alone do not contribute to CRC risk in the Hungarian population.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/7/54/prepub

Title: Common NOD2/CARD15 variants are not associated with susceptibility or the clinicopathologic characteristics of sporadic colorectal cancer in Hungarian patients
Authors: Peter Laszlo Lakatos
Erika Hitre
Ferenc Szalay
Kerstin Zinober
Peter Fuszek
Laszlo Lakatos
Simon Fischer
Janos Osztovits
Orsolya Gemela
Gabor Veres
Janos Papp
Peter Ferenci
Publication date: 01-12-2007
Publisher: BioMed Central
Published in: BMC Cancer / Issue 1/2007
Electronic ISSN: 1471-2407
DOI: https://doi.org/10.1186/1471-2407-7-54

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