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BMC Cancer
Published in: BMC Cancer 1/2006

Open Access 01-12-2006 | Research article

Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer

Authors: Rossella Solmi, Giampaolo Ugolini, Giancarlo Rosati, Simone Zanotti, Mattia Lauriola, Isacco Montroni, Marco del Governatore, Antonello Caira, Mario Taffurelli, Donatella Santini, Domenico Coppola, Lia Guidotti, Paolo Carinci, Pierluigi Strippoli

Published in: BMC Cancer | Issue 1/2006

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Abstract

Background

The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions.

Methods

In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription – polymerase chain reaction (RT-PCR).

Results

Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify.

Conclusion

The design of new approaches to identify such markers is warranted.
Appendix
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Metadata
Title
Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer
Authors
Rossella Solmi
Giampaolo Ugolini
Giancarlo Rosati
Simone Zanotti
Mattia Lauriola
Isacco Montroni
Marco del Governatore
Antonello Caira
Mario Taffurelli
Donatella Santini
Domenico Coppola
Lia Guidotti
Paolo Carinci
Pierluigi Strippoli
Publication date
01-12-2006
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2006
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-6-250

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