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BMC Cancer

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Open Access 01-12-2005 | Research article

Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa

Authors: Laura Kummola, Jonna M Hämäläinen, Jyrki Kivelä, Antti J Kivelä, Juha Saarnio, Tuomo Karttunen, Seppo Parkkila

Published in: BMC Cancer | Issue 1/2005

Abstract

Background

Carbonic anhydrase (CA) isozymes may have an important role in cancer development. Some isozymes control pH homeostasis in tumors that appears to modulate the behaviour of cancer cells. CA XIII is the newest member of the CA gene family. It is a cytosolic isozyme which is expressed in a number of normal tissues. The present study was designed to investigate CA XIII expression in prospectively collected colorectal tumor samples.

Methods

Both neoplastic and normal tissue specimens were obtained from the same patients. The analyses were performed using CA XIII-specific antibodies and an immunohistochemical staining method. For comparison, the tissue sections were immunostained for other cytosolic isozymes, CA I and II.

Results

The results indicated that the expression of CA XIII is down-regulated in tumor cells compared to the normal tissue. The lowest signal was detected in carcinoma samples. This pattern of expression was quite parallel for CA I and II.

Conclusion

The down-regulation of cytosolic CA I, II and XIII in colorectal cancer may result from reduced levels of a common transcription factor or loss of closely linked CA1, CA2 and CA13 alleles on chromosome 8. Their possible role as tumor suppressors should be further evaluated.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/5/41/prepub

Title: Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa
Authors: Laura Kummola
Jonna M Hämäläinen
Jyrki Kivelä
Antti J Kivelä
Juha Saarnio
Tuomo Karttunen
Seppo Parkkila
Publication date: 01-12-2005
Publisher: BioMed Central
Published in: BMC Cancer / Issue 1/2005
Electronic ISSN: 1471-2407
DOI: https://doi.org/10.1186/1471-2407-5-41

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