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BMC Cancer
Published in: BMC Cancer 1/2005

Open Access 01-12-2005 | Research article

Caffeic acid phenethyl ester decreases acute pneumonitis after irradiation in vitro and in vivo

Authors: Miao-Fen Chen, Peter C Keng, Paul-Yang Lin, Cheng-Ta Yang, Shuen-Kuei Liao, Wen-Cheng Chen

Published in: BMC Cancer | Issue 1/2005

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Abstract

Background

Lung cancer is relatively resistant to radiation treatment and radiation pneumonitis is a major obstacle to increasing the radiation dose. We previously showed that Caffeic acid phenethyl ester (CAPE) induces apoptosis and increases radiosensitivity in lung cancer. To determine whether CAPE, an antioxidant and an inhibitor of NF-kappa B, could be a useful adjuvant agent for lung cancer treatment, we examine the effects of CAPE on irradiated normal lung tissue in this study.

Methods

We compared the effects of CAPE on cytotoxicity and intracellular oxidative stress in normal lung fibroblast and a lung cancer cell line. For in vivo analysis, whole thorax radiation (single dose 10 Gy and 20 Gy) was delivered to BALB/c male mice with or without CAPE pretreatment. NF- kappaB activation and the expression levels of acute inflammatory cytokines were evaluated in mice after irradiation.

Results

The in vitro studies showed that CAPE cause no significant cytotoxicity in normal lung as compared to lung cancer cells. This is probably due to the differential effect on the expression of NF-kappa B between normal and malignant lung cells. The results from in vivo study showed that CAPE treatment decreased the expression of inflammatory cytokines including IL-1 alpha and beta, IL-6, TNF-alpha and TGF- beta, after irradiation. Moreover, histological and immunochemical data revealed that CAPE decreased radiation- induced interstitial pneumonitis and TGF-beta expression.

Conclusion

This study suggests that CAPE decreases the cascade of inflammatory responses induced by thoracic irradiation without causing toxicity in normal lung tissue. This provides a rationale for combining CAPE and thoracic radiotherapy for lung cancer treatment in further clinical studies.
Appendix
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Metadata
Title
Caffeic acid phenethyl ester decreases acute pneumonitis after irradiation in vitro and in vivo
Authors
Miao-Fen Chen
Peter C Keng
Paul-Yang Lin
Cheng-Ta Yang
Shuen-Kuei Liao
Wen-Cheng Chen
Publication date
01-12-2005
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2005
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-5-158

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