Published in:
Open Access
01-12-2013 | Research article
Decreased miR-106a inhibits glioma cell glucose uptake and proliferation by targeting SLC2A3 in GBM
Authors:
Dong-Wei Dai, Qiong Lu, Lai-Xing Wang, Wen-Yuan Zhao, Yi-Qun Cao, Ya-Nan Li, Guo-Sheng Han, Jian-Min Liu, Zhi-Jian Yue
Published in:
BMC Cancer
|
Issue 1/2013
Login to get access
Abstract
Background
MiR-106a is frequently down-regulated in various types of human cancer. However the underlying mechanism of miR-106a involved in glioma remains elusive.
Methods
The association of miR-106a with glioma grade and patient survival was analyzed. The biological function and target of miR-106a were determined by bioinformatic analysis and cell experiments (Western blot, luciferase reporter, cell cycle, ntracellular ATP production and glucose uptake assay). Finally, rescue expression of its target SLC2A3 was used to test the role of SLC2A3 in miR-106a-mediated cell glycolysis and proliferation.
Results
Here we showed that miR-106a was a tumor suppressor miRNA was involved in GBM cell glucose uptake and proliferation. Decreased miR-106a in GBM tissues and conferred a poor survival of GBM patients. SLC2A3 was identified as a core target of miR-106a in GBM cells. Inhibition of SLC2A3 by miR-106a attenuated cell proliferation and inhibited glucose uptake. In addition, for each biological process we identified ontology-associated transcripts that significantly correlated with SLC2A3 expression. Finally, the expression of SLC2A3 largely abrogated miR-106a-mediated cell proliferation and glucose uptake in GBM cells.
Conclusions
Taken together, miR-106a and SLC2A3 could be potential therapeutic approaches for GBM.