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Open Access 01-12-2013 | Research article

Expression of TRAIL-splice variants in gastric carcinomas: identification of TRAIL-γ as a prognostic marker

Authors: Andreas Krieg, Sabrina Mersch, Nadine Wolf, Nikolas H Stoecklein, Pablo E Verde, Jan Schulte am Esch 2nd, Sebastian Heikaus, Helmut E Gabbert, Wolfram T Knoefel, Csaba Mahotka

Published in: BMC Cancer | Issue 1/2013

Abstract

Background

TNF-related apoptosis inducing ligand (TRAIL) belongs to the TNF-superfamily that induces apoptotic cell death in a wide range of neoplastic cells in vivo as well as in vitro. We identified two alternative TRAIL-splice variants, i.e. TRAIL-β and TRAIL-γ that are characterized by the loss of their proapoptotic properties. Herein, we investigated the expression and the prognostic values of the TRAIL-splice variants in gastric carcinomas.

Methods

Real time PCR for amplification of the TRAIL-splice variants was performed in tumour tissue specimens and corresponding normal tissues of 41 consecutive patients with gastric carcinoma. Differences on mRNA-expression levels of the TRAIL-isoforms were compared to histo-pathological variables and correlated with survival data.

Results

All three TRAIL-splice variants could be detected in both non-malignant and malignant tissues, irrespective of their histological staging, grading or tumour types. However, TRAIL-β exhibited a higher expression in normal gastric tissue. The proapoptotic TRAIL-α expression was increased in gastric carcinomas when compared to TRAIL-β and TRAIL-γ. In addition, overexpression of TRAIL-γ was associated with a significant higher survival rate.

Conclusions

This is the first study that investigated the expression of TRAIL-splice variants in gastric carcinoma tissue samples. Thus, we provide first data that indicate a prognostic value for TRAIL-γ overexpression in this tumour entity.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/13/384/prepub

Title: Expression of TRAIL-splice variants in gastric carcinomas: identification of TRAIL-γ as a prognostic marker
Authors: Andreas Krieg
Sabrina Mersch
Nadine Wolf
Nikolas H Stoecklein
Pablo E Verde
Jan Schulte am Esch 2nd
Sebastian Heikaus
Helmut E Gabbert
Wolfram T Knoefel
Csaba Mahotka
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: BMC Cancer / Issue 1/2013
Electronic ISSN: 1471-2407
DOI: https://doi.org/10.1186/1471-2407-13-384

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