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BMC Cancer
Published in: BMC Cancer 1/2011

Open Access 01-12-2011 | Research article

MMP28 (epilysin) as a novel promoter of invasion and metastasis in gastric cancer

Authors: Pan Jian, Tao Yanfang, Zhou Zhuan, Wang Jian, Zhu Xueming, Ni Jian

Published in: BMC Cancer | Issue 1/2011

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Abstract

Background

The purpose of this study was to investigate invasion and metastasis related genes in gastric cancer.

Methods

The transwell migration assay was used to select a highly invasive sub-line from minimally invasive parent gastric cancer cells, and gene expression was compared using a microarray. MMP28 upregulation was confirmed using qRT-PCR. MMP28 immunohistochemistry was performed in normal and gastric cancer specimens. Invasiveness and tumor formation of stable cells overexpressing MMP28 were tested in vitro and in vivo.

Results

MMP28 was overexpressed in the highly invasive sub-cell line. Immunohistochemistry revealed MMP28 expression was markedly increased in gastric carcinoma relative to normal epithelia, and was significantly associated with depth of tumor invasion, lymph node metastasis and poorer overall survival. Ectopic expression of MMP28 indicated MMP28 promoted tumor cell invasion in vitro and increased gastric carcinoma metastasis in vivo.

Conclusions

This study indicates MMP28 is frequently overexpressed during progression of gastric carcinoma, and contributes to tumor cell invasion and metastasis. MMP28 may be a novel therapeutic target for prevention and treatment of metastases in gastric cancer.
Appendix
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Metadata
Title
MMP28 (epilysin) as a novel promoter of invasion and metastasis in gastric cancer
Authors
Pan Jian
Tao Yanfang
Zhou Zhuan
Wang Jian
Zhu Xueming
Ni Jian
Publication date
01-12-2011
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2011
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-11-200

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