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BMC Cancer
Published in: BMC Cancer 1/2010

Open Access 01-12-2010 | Research article

Prognostic relevance of Bmi-1 expression and autoantibodies in esophageal squamous cell carcinoma

Authors: Wan-li Liu, Xian-zhi Guo, Lan-jun Zhang, Jun-ye Wang, Ge Zhang, Su Guan, Yu-min Chen, Qing-li Kong, Li-hua Xu, Man-zhi Li, Li-bing Song, Mu-sheng Zeng

Published in: BMC Cancer | Issue 1/2010

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Abstract

Background

Overexpression of Bmi-1 has been observed in a variety of cancers, and it has been suggested to be an independent prognostic marker for the patients. The objective of this study was to determine the level of Bmi-1 expression or its autoantibodies in human esophageal squamous cell carcinoma (ESCC) and to correlate it with clinicopathologic data.

Methods

We first examined Bmi-1 expression in ESCC cell lines and tumor samples by RT-PCR and Western blot analysis. We then analyzed Bmi-1 protein expression in 171 clinicopathologically characterized ESCC cases by immunohistochemistry. In addition, we detected its autoantibodies in sera of patients with ESCC by ELISA.

Results

We found that Bmi-1 expression was higher in the immortalized cells, cancer cell lines and most cancer tissue than in non-tumorous control tissue at both mRNA and protein level. In addition, Bmi-1 expression was observed in 64.3% (110 of 171) archive ESCC specimen by immunohistochemistry analysis, and the location of Bmi-1 in ESCC was in the nuclei instead of cytoplasm of tumor cells. There was a significant difference of Bmi-1 expression in patients categorized according to stage (P = 0.003) and pN classification (P = 0.047). Multivariate analysis suggested that Bmi-1 expression was an independent prognostic marker for ESCC patients. A prognostic significance of Bmi-1 was also found in the subgroup of T3~T4 and N1 tumor classification. Bmi-1 autoantibodies were detected in sera of 39.0% (62 of 159) ESCC patients. The correlations between anti-Bmi-1 antibodies and tumor stage (P = 0.040), or lymph node status (P < 0.001) were significant.

Conclusions

Our results suggest that Bmi-1 protein is a valuable marker of ESCC progression. The presence of Bmi-1 autoantibodies in sera from patients with ESCC may have clinical utility in esophageal cancer diagnosis.
Appendix
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Metadata
Title
Prognostic relevance of Bmi-1 expression and autoantibodies in esophageal squamous cell carcinoma
Authors
Wan-li Liu
Xian-zhi Guo
Lan-jun Zhang
Jun-ye Wang
Ge Zhang
Su Guan
Yu-min Chen
Qing-li Kong
Li-hua Xu
Man-zhi Li
Li-bing Song
Mu-sheng Zeng
Publication date
01-12-2010
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2010
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-10-467

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