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BMC Cancer
Published in: BMC Cancer 1/2010

Open Access 01-12-2010 | Research article

An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer

Authors: Iker Sánchez-Navarro, Angelo Gámez-Pozo, Álvaro Pinto, David Hardisson, Rosario Madero, Rocío López, Belén San José, Pilar Zamora, Andrés Redondo, Jaime Feliu, Paloma Cejas, Manuel González Barón, Juan Ángel Fresno Vara, Enrique Espinosa

Published in: BMC Cancer | Issue 1/2010

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Abstract

Background

Gene expression profiling may improve prognostic accuracy in patients with early breast cancer. Our objective was to demonstrate that it is possible to develop a simple molecular signature to predict distant relapse.

Methods

We included 153 patients with stage I-II hormonal receptor-positive breast cancer. RNA was isolated from formalin-fixed paraffin-embedded samples and qRT-PCR amplification of 83 genes was performed with gene expression assays. The genes we analyzed were those included in the 70-Gene Signature, the Recurrence Score and the Two-Gene Index. The association among gene expression, clinical variables and distant metastasis-free survival was analyzed using Cox regression models.

Results

An 8-gene prognostic score was defined. Distant metastasis-free survival at 5 years was 97% for patients defined as low-risk by the prognostic score versus 60% for patients defined as high-risk. The 8-gene score remained a significant factor in multivariate analysis and its performance was similar to that of two validated gene profiles: the 70-Gene Signature and the Recurrence Score. The validity of the signature was verified in independent cohorts obtained from the GEO database.

Conclusions

This study identifies a simple gene expression score that complements histopathological prognostic factors in breast cancer, and can be determined in paraffin-embedded samples.
Appendix
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Metadata
Title
An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer
Authors
Iker Sánchez-Navarro
Angelo Gámez-Pozo
Álvaro Pinto
David Hardisson
Rosario Madero
Rocío López
Belén San José
Pilar Zamora
Andrés Redondo
Jaime Feliu
Paloma Cejas
Manuel González Barón
Juan Ángel Fresno Vara
Enrique Espinosa
Publication date
01-12-2010
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2010
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-10-336

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