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Open Access 01-12-2012 | Research article

Dexmedetomidine is neuroprotective in an in vitro model for traumatic brain injury

Authors: Marc Schoeler, Philip D Loetscher, Rolf Rossaint, Astrid V Fahlenkamp, Georg Eberhardt, Steffen Rex, Joachim Weis, Mark Coburn

Published in: BMC Neurology | Issue 1/2012

Abstract

Background

The α₂-adrenoreceptor agonist dexmedetomidine is known to provide neuroprotection under ischemic conditions. In this study we investigated whether dexmedetomidine has a protective effect in an in vitro model for traumatic brain injury.

Methods

Organotypic hippocampal slice cultures were subjected to a focal mechanical trauma and then exposed to varying concentrations of dexmedetomidine. After 72 h cell injury was assessed using propidium iodide. In addition, the effects of delayed dexmedetomidine application, of hypothermia and canonical signalling pathway inhibitors were examined.

Results

Dexmedetomidine showed a protective effect on traumatically injured hippocampal cells with a maximum effect at a dosage of 1 μM. This effect was partially reversed by the simultaneous administration of the ERK inhibitor PD98059.

Conclusion

In this TBI model dexmedetomidine had a significant neuroprotective effect. Our results indicate that activation of ERK might be involved in mediating this effect.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2377/12/20/prepub

Title: Dexmedetomidine is neuroprotective in an in vitro model for traumatic brain injury
Authors: Marc Schoeler
Philip D Loetscher
Rolf Rossaint
Astrid V Fahlenkamp
Georg Eberhardt
Steffen Rex
Joachim Weis
Mark Coburn
Publication date: 01-12-2012
Publisher: BioMed Central
Published in: BMC Neurology / Issue 1/2012
Electronic ISSN: 1471-2377
DOI: https://doi.org/10.1186/1471-2377-12-20

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Dexmedetomidine is neuroprotective in an in vitro model for traumatic brain injury

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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