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Respiratory Research
Published in: Respiratory Research 1/2005

Open Access 01-12-2005 | Research

Uncoordinated production of Laminin-5 chains in airways epithelium of allergic asthmatics

Authors: Kawa Amin, Christer Janson, Lahja Sevéus, Kaoru Miyazaki, Ismo Virtanen, Per Venge

Published in: Respiratory Research | Issue 1/2005

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Abstract

Background

Laminins are a group of proteins largely responsible for the anchorage of cells to basement membranes. We hypothesized that altered Laminin chain production in the bronchial mucosa might explain the phenomenon of epithelial cell shedding in asthma. The aim was to characterize the presence of Laminin chains in the SEBM and epithelium in allergic and non-allergic asthmatics.

Patients and methods

Biopsies were taken from the bronchi of 11 patients with allergic and 9 patients with non-allergic asthma and from 7 controls and stained with antibodies against the Laminin (ln) chains alpha1-alpha5, beta1-beta2 and gamma1-gamma2.

Results

Lns-2,-5 and -10 were the main Laminins of SEBM. The layer of ln-10 was thicker in the two asthmatic groups while an increased thickness of lns-2 and -5 was only seen in allergic asthmatics. The ln gamma2-chain, which is only found in ln 5, was exclusively expressed in epithelial cells in association with epithelial injury and in the columnar epithelium of allergic asthmatics.

Conclusion

The uncoordinated production of chains of ln-5 in allergic asthma could have a bearing on the poor epithelial cell anchorage in these patients.
Literature
1.
2.
3.
Amin K, Ludviksdottir D, Janson C, Nettelbladt O, Bjornsson E, Roomans GM, Boman G, Seveus L, Venge P: Inflammation and structural changes in the airways of patients with atopic and nonatopic asthma. Am J Respir Crit Care Med 2000, 162:2295–2301.CrossRefPubMed
4.
Patarroyo M, Tryggvason K, Virtanen I: Laminin isoforms in tumor invasion, angiogenesis and metastasis. Semin Cancer Biol 2002, 12:197–207.CrossRefPubMed
5.
Libby RT, Champliaud MF, Claudepierre T, Xu Y, Gibbons EP, Koch M, Burgeson RE, Hunter DD, Brunken WJ: Laminin expression in adult and developing retinae: evidence of two novel CNS laminins. J Neurosci 2000, 20:6517–6528.PubMedPubMedCentral
6.
Kikkawa Y, Moulson CL, Virtanen I, Miner JH: Identification of the binding site for the Lutheran blood group glycoprotein on laminin alpha 5 through expression of chimeric laminin chains in vivo. J Biol Chem 2002, 277:44864–44869.CrossRefPubMed
7.
8.
Fireman P: Understanding asthma pathophysiology. Allergy Asthma Proc 2003, 24:79–83.PubMed
9.
Ludviksdottir D, Janson C, Bjornsson E, Stalenheim G, Boman G, Hedenstrom H, Venge P, Gudbjornsson B, Valtysdottir S: Different airway responsiveness profiles in atopic asthma, nonatopic asthma, and Sjogren's syndrome. BHR Study Group. Bronchial hyperresponsiveness. Allergy 2000, 55:259–265.CrossRefPubMed
10.
Crimi E, Spanevello A, Neri M, Ind PW, Rossi GA, Brusasco V: Dissociation between airway inflammation and airway hyperresponsiveness in allergic asthma. Am J Respir Crit Care Med 1998, 157:4–9.CrossRefPubMed
11.
Kushima A, Motojima S, Yamai T, Makino S: The participation of epithelial desquamation in the increase of bronchial hyperresponsiveness after antigen challenge in patients with bronchial asthma. Allergy (Japan) 1990, 39 No.12:1581–1589.
12.
Virtanen I, Gullberg D, Rissanen J, Kivilaakso E, Kiviluoto T, Laitinen LA, Lehto VP, Ekblom P: Laminin alpha1-chain shows a restricted distribution in epithelial basement membranes of fetal and adult human tissues. Exp Cell Res 2000, 257:298–309.CrossRefPubMed
13.
Petajaniemi N, Korhonen M, Kortesmaa J, Tryggvason K, Sekiguchi K, Fujiwara H, Sorokin L, Thornell LE, Wondimu Z, Assefa D, Patarroyo M, Virtanen I: Localization of laminin alpha4-chain in developing and adult human tissues. J Histochem Cytochem 2002, 50:1113–1130.CrossRefPubMed
14.
Engvall E, Earwicker D, Haaparanta T, Ruoslahti E, Sanes JR: Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits. Cell Regul 1990, 1:731–740.PubMedPubMedCentral
15.
Tiger CF, Champliaud MF, Pedrosa-Domellof F, Thornell LE, Ekblom P, Gullberg D: Presence of laminin alpha5 chain and lack of laminin alpha1 chain during human muscle development and in muscular dystrophies. J Biol Chem 1997, 272:28590–28595.CrossRefPubMed
16.
Virtanen I, Lohi J, Tani T, Korhonen M, Burgeson RE, Lehto VP, Leivo I: Distinct changes in the laminin composition of basement membranes in human seminiferous tubules during development and degeneration. Am J Pathol 1997, 150:1421–1431.PubMedPubMedCentral
17.
Mizushima H, Koshikawa N, Moriyama K, Takamura H, Nagashima Y, Hirahara F, Miyazaki K: Wide distribution of laminin-5 gamma 2 chain in basement membranes of various human tissues. Horm Res 1998, 50 Suppl 2:7–14.PubMed
18.
Tunggal P, Smyth N, Paulsson M, Ott MC: Laminins: structure and genetic regulation. Microsc Res Tech 2000, 51:214–227.CrossRefPubMed
19.
Hintermann E, Quaranta V: Epithelial cell motility on laminin-5: regulation by matrix assembly, proteolysis, integrins and erbB receptors. Matrix Biol 2004, 23:75–85.CrossRefPubMed
20.
21.
Lappi-Blanco E, Kaarteenaho-Wiik R, Salo S, Sormunen R, Maatta M, Autio-Harmainen H, Soini Y, Paakko P: Laminin-5 gamma2 chain in cryptogenic organizing pneumonia and idiopathic pulmonary fibrosis. Am J Respir Crit Care Med 2004, 169:27–33.CrossRefPubMed
22.
Virtanen I, Laitinen A, Tani T, Paakko P, Laitinen LA, Burgeson RE, Lehto VP: Differential expression of laminins and their integrin receptors in developing and adult human lung. Am J Respir Cell Mol Biol 1996, 15:184–196.CrossRefPubMed
23.
24.
Altraja A, Laitinen A, Virtanen I, Kämpe M, Simonsson BG, Karlsson SE, Håkansson L, Venge P, Sillastu H, Laitinen LA: Expression of laminins in the airways in various types of asthmatic patients: A morphometric study. Am J Respir Cell Mol Biol 1996, 15:482–488.CrossRefPubMed
25.
Michelson PH, Tigue M, Jones JC: Human bronchial epithelial cells secrete laminin 5, express hemidesmosomal proteins, and assemble hemidesmosomes. J Histochem Cytochem 2000, 48:535–544.CrossRefPubMed
26.
Ghosh S, Stack MS: Proteolytic modification of laminins: functional consequences. Microsc Res Tech 2000, 51:238–246.CrossRefPubMed
Metadata
Title
Uncoordinated production of Laminin-5 chains in airways epithelium of allergic asthmatics
Authors
Kawa Amin
Christer Janson
Lahja Sevéus
Kaoru Miyazaki
Ismo Virtanen
Per Venge
Publication date
01-12-2005
Publisher
BioMed Central
Published in
Respiratory Research / Issue 1/2005
Electronic ISSN: 1465-993X
DOI
https://doi.org/10.1186/1465-9921-6-110

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