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Published in:

01-04-2017

Pseudo-typed Semliki Forest virus delivers EGFP into neurons

Authors: Fan Jia, Huan Miao, Xutao Zhu, Fuqiang Xu

Published in: Journal of NeuroVirology | Issue 2/2017

Abstract

Semliki Forest virus (SFV), a neurotropic virus, has been used to deliver heterologous genes into cells in vitro and in vivo. In this study, we constructed a reporter SFV4-FL-EGFP and found that it can deliver EGFP into neurons located at the injection site without disseminating throughout the brain. Lacking of the capsid gene of SFV4-FL-EGFP does not block its life cycle, while forming replication-competent virus-like particles (VLPs). These VLPs hold subviral genome by using the packaging sequence (PS) located within the nsP2 gene, and can transfer their genome into cells. In addition, we found that the G protein of vesicular stomatitis virus (VSVG) can package SFV subviral genome, which is consistent with the previous reports. The G protein of rabies virus (RVG) could also package SFV subviral genome. These pseudo-typed SFV can deliver EGFP gene into neurons. Taken together, these findings may be used to construct various SFV-based delivery systems for virological studies, gene therapy, and neural circuit labeling.

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Title: Pseudo-typed Semliki Forest virus delivers EGFP into neurons
Authors: Fan Jia
Huan Miao
Xutao Zhu
Fuqiang Xu
Publication date: 01-04-2017
Publisher: Springer International Publishing
Published in: Journal of NeuroVirology / Issue 2/2017
Print ISSN: 1355-0284
Electronic ISSN: 1538-2443
DOI: https://doi.org/10.1007/s13365-016-0486-8

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Pseudo-typed Semliki Forest virus delivers EGFP into neurons

Abstract

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